##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AccIII AclI AgeI ApaI AscI
AviII AvrII BamHI BlnI BsaBI
BseAI BsiEI BsiWI BsmBI BspDI
BspEI BsrBI BsrDI BsrFI BssHII
BstBI BstEII BstPI Cfr10I ClaI
CpoI EagI EclXI Eco52I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HpaI KspI MamI MroI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshAI Psp1406I PvuI RsrII
SacII SalI SbfI SexAI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
StuI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 1767 GACGTC GACGT^C
Acc65I 883 GGTACC G^GTACC
AocI 993 CCTCAGG CC^T[ACGT]AGG
Aor51HI 778 AGCGCT AGC^GCT
AseI 5071 ATTAAT AT^TAAT
AsnI 5071 ATTAAT AT^TAAT
Asp700I 4194 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 883 GGTACC G^GTACC
AspI 3540 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 3023 GGCGCC GGCGC^C
BbvCI 3531 CCTCAGC CCTCAGC(-5/-2)
BcgI 1809 GCAGCAGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1132 GCCTCACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 48 ACCGGA [AT]^CCGG[AT]
BsmI 1019 GCATTC GAATGC(1/-1)
Bsp1407I 847 TGTACA T^GTACA
BspLU11I 2987 ACATGT A^CATGT
BsrGI 847 TGTACA T^GTACA
Bst1107I 2223 GTATAC GTA^TAC
BstXI 3566 CCATGGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2223 GTATAC GTA^TAC
Bsu36I 993 CCTCAGG CC^T[ACGT]AGG
Eco47III 778 AGCGCT AGC^GCT
Eco81I 993 CCTCAGG CC^T[ACGT]AGG
KasI 3023 GGCGCC G^GCGCC
KpnI 883 GGTACC GGTAC^C
MfeI 1299 CAATTG C^AATTG
MluI 2174 ACGCGT A^CGCGT
MunI 1299 CAATTG C^AATTG
NarI 3023 GGCGCC GG^CGCC
PflFI 3540 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 5071 ATTAAT AT^TAAT
PstI 3844 CTGCAG CTGCA^G
ScaI 1154 AGTACT AGT^ACT
SfoI 3023 GGCGCC GGC^GCC
SphI 2691 GCATGC GCATG^C
SspBI 847 TGTACA T^GTACA
Tth111I 3540 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 4194 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AcyI 1767 GACGTC G[GA]^CG[CT]C
3023 GGCGCC
AflII 901 CTTAAG C^TTAAG
1494 CTTAAG
Alw44I 433 GTGCAC G^TGCAC
2524 GTGCAC
AlwNI 740 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
2320 CAGCTCCTG
ApaLI 433 GTGCAC G^TGCAC
2524 GTGCAC
BanI 883 GGTACC G^G[CT][GA]CC
3023 GGCGCC
BbrPI 1166 CACGTG CAC^GTG
2395 CACGTG
BclI 3089 TGATCA T^GATCA
5041 TGATCA
BfrI 901 CTTAAG C^TTAAG
1494 CTTAAG
BglII 282 AGATCT A^GATCT
2294 AGATCT
BsaAI 1166 CACGTG [CT]AC^GT[GA]
2395 CACGTG
BsaHI 1767 GACGTC G[GA]^CG[CT]C
3023 GGCGCC
BspHI 1862 TCATGA T^CATGA
5044 TCATGA
BspMI 645 GCAGGT ACCTGC(4/8)
1083 GCAGGT
BssSI 2416 CACGAG CACGAG(-5/-1)
1817 CTCGTG
DraI 3822 TTTAAA TTT^AAA
4522 TTTAAA
DraIII 2416 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
4630 CACAGTGTG
DrdI 1468 GACTTTATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2173 GACGCGTTGGTC
EcoNI 2016 CCTTCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3030 CCTAGAAGAGG
FbaI 3089 TGATCA T^GATCA
5041 TGATCA
Hin1I 1767 GACGTC G[GA]^CG[CT]C
3023 GGCGCC
HindIII 1927 AAGCTT A^AGCTT
4737 AAGCTT
NcoI 3566 CCATGG C^CATGG
3966 CCATGG
PmaCI 1166 CACGTG CAC^GTG
2395 CACGTG
PmlI 1166 CACGTG CAC^GTG
2395 CACGTG
RcaI 1862 TCATGA T^CATGA
5044 TCATGA
SacI 700 GAGCTC GAGCT^C
1513 GAGCTC
SapI 1194 GAAGAGC GCTCTTC(1/4)
1510 GAAGAGC
SmaI 2444 CCCGGG CCC^GGG
3935 CCCGGG
SmlI 901 CTTAAG C^T[CT][GA]AG
1494 CTTAAG
XbaI 1290 TCTAGA T^CTAGA
4472 TCTAGA
XcmI 1568 CCAAAGCACCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2240 CCAGGGAGCAGCTGG
XmaCI 2444 CCCGGG C^CCGGG
3935 CCCGGG
XmaI 2444 CCCGGG C^CCGGG
3935 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AhdI AluI AlwI ApoI
AspEI AspHI AvaI AvaII BalI
BanII BbsI BbvI BciVI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI Eam1105I EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII