##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
Acc65I AccIII AclI AgeI Alw44I
ApaLI AscI AseI AsnI Asp718I
AviII BamHI BbvCI BcgI BclI
BlpI Bpu1102I BsaI BsaWI BseAI
BsiEI BsiWI BspDI BspEI BspLU11I
BsrBI BssHII BstEII BstPI CelII
ClaI CpoI DraIII DrdI EagI
EclXI Eco52I EcoO65I EcoRI FbaI
FseI FspI KpnI MluI MroI
NdeI NotI NruI PacI PflMI
PinAI PmeI PshBI Psp1406I PvuI
RsrII SalI SbfI SfiI SgrAI
SmaI SnaBI SphI Sse8387I SspI
StuI SwaI Van91I XbaI XcmI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccI 425 GTATAC GT^[AC][GT]AC
AflII 4632 CTTAAG C^TTAAG
AocI 2835 CCTCAGG CC^T[ACGT]AGG
Aor51HI 4672 AGCGCT AGC^GCT
ApaI 1459 GGGCCC GGGCC^C
AvrII 5375 CCTAGG C^CTAGG
BfrI 4632 CTTAAG C^TTAAG
BglII 302 AGATCT A^GATCT
BlnI 5375 CCTAGG C^CTAGG
BsaBI 1962 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspHI 4663 TCATGA T^CATGA
BsrFI 2104 GCCGGC [GA]^CCGG[CT]
Bst1107I 425 GTATAC GTA^TAC
BstBI 46 TTCGAA TT^CGAA
BstZ17I 425 GTATAC GTA^TAC
Bsu36I 2835 CCTCAGG CC^T[ACGT]AGG
Cfr10I 2104 GCCGGC [GA]^CCGG[CT]
Eco47III 4672 AGCGCT AGC^GCT
Eco81I 2835 CCTCAGG CC^T[ACGT]AGG
EcoNI 2271 CCTCCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 4245 ATGCAT ATGCA^T
HpaI 3083 GTTAAC GTT^AAC
KspI 3755 CCGCGG CCGC^GG
MamI 1962 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 2104 GCCGGC GCC^GGC
NcoI 1324 CCATGG C^CATGG
NgoMIV 2104 GCCGGC G^CCGGC
NheI 4679 GCTAGC G^CTAGC
NsiI 4245 ATGCAT ATGCA^T
NspV 46 TTCGAA TT^CGAA
PaeR7I 3360 CTCGAG C^TCGAG
PshAI 441 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 4663 TCATGA T^CATGA
SacII 3755 CCGCGG CCGC^GG
SapI 1411 GCTCTTC GCTCTTC(1/4)
ScaI 1890 AGTACT AGT^ACT
SfuI 46 TTCGAA TT^CGAA
SpeI 3935 ACTAGT A^CTAGT
XhoI 3360 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 2371 CGCG CG^CG
3756 CGCG
AhdI 175 GACTCGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
993 GACAAGACGTC
AspEI 175 GACTCGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
993 GACAAGACGTC
AvaI 3360 CTCGAG C^[CT]CG[GA]G
4079 CTCGGG
BbeI 673 GGCGCC GGCGC^C
3994 GGCGCC
BcnI 79 CCCGG CC^[GC]GG
1456 CCGGG
BglI 2026 GCCGAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3051 GCCAGTGGGGC
BsmBI 2006 GAGACG CGTCTC(1/5)
3117 GAGACG
BsmI 2642 GAATGC GAATGC(1/-1)
4494 GCATTC
BsoBI 3360 CTCGAG C^[CT]CG[GA]G
4079 CTCGGG
Bsp1407I 2423 TGTACA T^GTACA
3542 TGTACA
BsrGI 2423 TGTACA T^GTACA
3542 TGTACA
BstAPI 135 GCAAAAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3147 GCATTTGGTGC
BstUI 2371 CGCG CG^CG
3756 CGCG
Eam1105I 175 GACTCGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
993 GACAAGACGTC
EcoRV 1319 GATATC GAT^ATC
2936 GATATC
HgaI 560 GACGC GACGC(5/10)
3156 GCGTC
KasI 673 GGCGCC G^GCGCC
3994 GGCGCC
MfeI 1159 CAATTG C^AATTG
4712 CAATTG
MunI 1159 CAATTG C^AATTG
4712 CAATTG
MvnI 2371 CGCG CG^CG
3756 CGCG
NarI 673 GGCGCC GG^CGCC
3994 GGCGCC
NciI 79 CCCGG CC^[GC]GG
1456 CCGGG
PvuII 1877 CAGCTG CAG^CTG
4156 CAGCTG
SexAI 1688 ACCTGGT A^CC[AT]GGT
2228 ACCTGGT
SfoI 673 GGCGCC GGC^GCC
3994 GGCGCC
SspBI 2423 TGTACA T^GTACA
3542 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AatII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
Asp700I AspHI AspI AvaII BalI
BanI BanII BbrPI BbsI BbvI
BciVI BfaI BmyI BpmI BpuAI
BsaAI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrDI BsrI BssKI
BssSI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PflFI
PleI PmaCI PmlI PpuMI PstI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I XhoII XmnI