################################## # NO CUT ENZYMES ( 72 enzymes) # ################################## Acc65I AccIII AclI AgeI Alw44I ApaLI AscI AseI AsnI Asp718I AviII BamHI BbvCI BcgI BclI BlpI Bpu1102I BsaI BsaWI BseAI BsiEI BsiWI BspDI BspEI BspLU11I BsrBI BssHII BstEII BstPI CelII ClaI CpoI DraIII DrdI EagI EclXI Eco52I EcoO65I EcoRI FbaI FseI FspI KpnI MluI MroI NdeI NotI NruI PacI PflMI PinAI PmeI PshBI Psp1406I PvuI RsrII SalI SbfI SfiI SgrAI SmaI SnaBI SphI Sse8387I SspI StuI SwaI Van91I XbaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AccI 425 GTATAC GT^[AC][GT]AC AflII 4632 CTTAAG C^TTAAG AocI 2835 CCTCAGG CC^T[ACGT]AGG Aor51HI 4672 AGCGCT AGC^GCT ApaI 1459 GGGCCC GGGCC^C AvrII 5375 CCTAGG C^CTAGG BfrI 4632 CTTAAG C^TTAAG BglII 302 AGATCT A^GATCT BlnI 5375 CCTAGG C^CTAGG BsaBI 1962 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BspHI 4663 TCATGA T^CATGA BsrFI 2104 GCCGGC [GA]^CCGG[CT] Bst1107I 425 GTATAC GTA^TAC BstBI 46 TTCGAA TT^CGAA BstZ17I 425 GTATAC GTA^TAC Bsu36I 2835 CCTCAGG CC^T[ACGT]AGG Cfr10I 2104 GCCGGC [GA]^CCGG[CT] Eco47III 4672 AGCGCT AGC^GCT Eco81I 2835 CCTCAGG CC^T[ACGT]AGG EcoNI 2271 CCTCCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 4245 ATGCAT ATGCA^T HpaI 3083 GTTAAC GTT^AAC KspI 3755 CCGCGG CCGC^GG MamI 1962 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NaeI 2104 GCCGGC GCC^GGC NcoI 1324 CCATGG C^CATGG NgoMIV 2104 GCCGGC G^CCGGC NheI 4679 GCTAGC G^CTAGC NsiI 4245 ATGCAT ATGCA^T NspV 46 TTCGAA TT^CGAA PaeR7I 3360 CTCGAG C^TCGAG PshAI 441 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 4663 TCATGA T^CATGA SacII 3755 CCGCGG CCGC^GG SapI 1411 GCTCTTC GCTCTTC(1/4) ScaI 1890 AGTACT AGT^ACT SfuI 46 TTCGAA TT^CGAA SpeI 3935 ACTAGT A^CTAGT XhoI 3360 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccII 2371 CGCG CG^CG 3756 CGCG AhdI 175 GACTCGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 993 GACAAGACGTC AspEI 175 GACTCGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 993 GACAAGACGTC AvaI 3360 CTCGAG C^[CT]CG[GA]G 4079 CTCGGG BbeI 673 GGCGCC GGCGC^C 3994 GGCGCC BcnI 79 CCCGG CC^[GC]GG 1456 CCGGG BglI 2026 GCCGAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3051 GCCAGTGGGGC BsmBI 2006 GAGACG CGTCTC(1/5) 3117 GAGACG BsmI 2642 GAATGC GAATGC(1/-1) 4494 GCATTC BsoBI 3360 CTCGAG C^[CT]CG[GA]G 4079 CTCGGG Bsp1407I 2423 TGTACA T^GTACA 3542 TGTACA BsrGI 2423 TGTACA T^GTACA 3542 TGTACA BstAPI 135 GCAAAAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3147 GCATTTGGTGC BstUI 2371 CGCG CG^CG 3756 CGCG Eam1105I 175 GACTCGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 993 GACAAGACGTC EcoRV 1319 GATATC GAT^ATC 2936 GATATC HgaI 560 GACGC GACGC(5/10) 3156 GCGTC KasI 673 GGCGCC G^GCGCC 3994 GGCGCC MfeI 1159 CAATTG C^AATTG 4712 CAATTG MunI 1159 CAATTG C^AATTG 4712 CAATTG MvnI 2371 CGCG CG^CG 3756 CGCG NarI 673 GGCGCC GG^CGCC 3994 GGCGCC NciI 79 CCCGG CC^[GC]GG 1456 CCGGG PvuII 1877 CAGCTG CAG^CTG 4156 CAGCTG SexAI 1688 ACCTGGT A^CC[AT]GGT 2228 ACCTGGT SfoI 673 GGCGCC GGC^GCC 3994 GGCGCC SspBI 2423 TGTACA T^GTACA 3542 TGTACA ######################################### # 3 OR MORE CUT ENZYMES ( 120 enzymes) # ######################################### AatII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI ApoI Asp700I AspHI AspI AvaII BalI BanI BanII BbrPI BbsI BbvI BciVI BfaI BmyI BpmI BpuAI BsaAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrDI BsrI BssKI BssSI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PflFI PleI PmaCI PmlI PpuMI PstI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XhoII XmnI