##################################
# NO CUT ENZYMES ( 48 enzymes) #
##################################
AclI AgeI Aor51HI ApaI AscI
AviII AvrII BbvCI BcgI BlnI
BsiWI BsmBI BspDI Bst1107I BstBI
BstZ17I ClaI CpoI DrdI Eco47III
EcoRI EcoRV FseI FspI HpaI
MfeI MluI MunI NotI NruI
NspV PaeR7I PinAI PmeI Psp1406I
RsrII SapI SbfI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SwaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AatII 3314 GACGTC GACGT^C
Acc65I 33 GGTACC G^GTACC
AccIII 847 TCCGGA T^CCGGA
AflII 3418 CTTAAG C^TTAAG
AhdI 3079 GACCAGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2236 CCTCAGG CC^T[ACGT]AGG
Asp718I 33 GGTACC G^GTACC
AspEI 3079 GACCAGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 483 GGCGCC GGCGC^C
BfrI 3418 CTTAAG C^TTAAG
BsaBI 1055 GATCCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 847 TCCGGA [AT]^CCGG[AT]
BseAI 847 TCCGGA T^CCGGA
BsgI 2678 CTGCAC GTGCAG(16/14)
Bsp1407I 2108 TGTACA T^GTACA
BspEI 847 TCCGGA T^CCGGA
BspHI 1533 TCATGA T^CATGA
BspLU11I 3125 ACATGT A^CATGT
BsrBI 4599 GAGCGG CCGCTC(-3/-3)
BsrGI 2108 TGTACA T^GTACA
BssHII 503 GCGCGC G^CGCGC
BssSI 1077 CACGAG CACGAG(-5/-1)
BstAPI 1267 GCATCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 2236 CCTCAGG CC^T[ACGT]AGG
DraIII 3496 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 3079 GACCAGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2236 CCTCAGG CC^T[ACGT]AGG
HaeII 483 GGCGCC [GA]GCGC^[CT]
KasI 483 GGCGCC G^GCGCC
KpnI 33 GGTACC GGTAC^C
MamI 1055 GATCCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 847 TCCGGA T^CCGGA
NarI 483 GGCGCC GG^CGCC
NdeI 1540 CATATG CA^TATG
PacI 4715 TTAATTAA TTAAT^TAA
PflMI 2695 CCAGACCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1471 GACTTACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 42 CGATCG CGAT^CG
PvuII 4173 CAGCTG CAG^CTG
RcaI 1533 TCATGA T^CATGA
SalI 3317 GTCGAC G^TCGAC
ScaI 3474 AGTACT AGT^ACT
SexAI 2760 ACCAGGT A^CC[AT]GGT
SfoI 483 GGCGCC GGC^GCC
SphI 3305 GCATGC GCATG^C
SspBI 2108 TGTACA T^GTACA
StuI 1191 AGGCCT AGG^CCT
Van91I 2695 CCAGACCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 3740 CCAGAGATCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 42 enzymes) #
###################################
AccI 3317 GTCGAC GT^[AC][GT]AC
4761 GTCTAC
AflIII 3125 ACATGT A^C[GA][CT]GT
3145 ACACGT
AspI 1301 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4562 GACACTGTC
AvaI 3926 CCCGAG C^[CT]CG[GA]G
4068 CTCGGG
BalI 1165 TGGCCA TGG^CCA
3180 TGGCCA
BamHI 1054 GGATCC G^GATCC
4770 GGATCC
BbrPI 254 CACGTG CAC^GTG
3146 CACGTG
BclI 723 TGATCA T^GATCA
1536 TGATCA
BglI 621 GCCTCCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1184 GCCATGAAGGC
BglII 3583 AGATCT A^GATCT
3744 AGATCT
BlpI 790 GCTCAGC GC^T[ACGT]AGC
4449 GCTAAGC
Bpu1102I 790 GCTCAGC GC^T[ACGT]AGC
4449 GCTAAGC
BsoBI 3926 CCCGAG C^[CT]CG[GA]G
4068 CTCGGG
BspMI 1027 GCAGGT ACCTGC(4/8)
1546 GCAGGT
BstEII 67 GGTCACC G^GT[ACGT]ACC
3692 GGTCACC
BstPI 67 GGTCACC G^GT[ACGT]ACC
3692 GGTCACC
CelII 790 GCTCAGC GC^T[ACGT]AGC
4449 GCTAAGC
DraI 2998 TTTAAA TTT^AAA
4780 TTTAAA
EagI 136 CGGCCG C^GGCCG
770 CGGCCG
EclXI 136 CGGCCG C^GGCCG
770 CGGCCG
Eco52I 136 CGGCCG C^GGCCG
770 CGGCCG
EcoNI 825 CCTTCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3290 CCTGTTTGAGG
EcoO65I 67 GGTCACC G^GT[ACGT]ACC
3692 GGTCACC
EcoT22I 710 ATGCAT ATGCA^T
1265 ATGCAT
FbaI 723 TGATCA T^GATCA
1536 TGATCA
KspI 310 CCGCGG CCGC^GG
631 CCGCGG
MluNI 1165 TGGCCA TGG^CCA
3180 TGGCCA
MscI 1165 TGGCCA TGG^CCA
3180 TGGCCA
NaeI 218 GCCGGC GCC^GGC
239 GCCGGC
NcoI 1909 CCATGG C^CATGG
4062 CCATGG
NgoMIV 218 GCCGGC G^CCGGC
239 GCCGGC
NsiI 710 ATGCAT ATGCA^T
1265 ATGCAT
PflFI 1301 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4562 GACACTGTC
PmaCI 254 CACGTG CAC^GTG
3146 CACGTG
PmlI 254 CACGTG CAC^GTG
3146 CACGTG
PstI 1448 CTGCAG CTGCA^G
3109 CTGCAG
SacI 411 GAGCTC GAGCT^C
1996 GAGCTC
SacII 310 CCGCGG CCGC^GG
631 CCGCGG
SfiI 620 GGCCTCCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1183 GGCCATGAAGGCC
SmlI 3418 CTTAAG C^T[CT][GA]AG
4011 CTTGAG
Tth111I 1301 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4562 GACACTGTC
XbaI 3281 TCTAGA T^CTAGA
4410 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI Alw44I AlwI AlwNI ApaLI
ApoI AseI AsnI Asp700I AspHI
AvaII BanI BanII BbsI BbvI
BciVI BcnI BfaI BmyI BpmI
BpuAI BsaAI BsaHI BsaI BsaJI
BseRI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NheI
NlaIII NlaIV NspI PleI PpuMI
PshBI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI