################################## # NO CUT ENZYMES ( 64 enzymes) # ################################## AatII AccI AccIII AclI AflII AgeI Alw44I AocI ApaLI AscI AvrII BalI BamHI BbeI BcgI BclI BfrI BlnI BseAI BsiWI BsmBI BspDI BspEI BspHI Bst1107I BstEII BstPI BstZ17I Bsu36I ClaI CpoI EagI EclXI Eco52I Eco81I EcoO65I FbaI FseI KasI MluNI MroI MscI NaeI NarI NdeI NgoMIV NotI NruI PacI PinAI PmeI Psp1406I RcaI RsrII SalI SbfI SexAI SfiI SfoI SnaBI SpeI SphI Sse8387I SwaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### Acc65I 562 GGTACC G^GTACC AhdI 1484 GACGAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 1867 AGCGCT AGC^GCT Asp718I 562 GGTACC G^GTACC AspEI 1484 GACGAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbrPI 1416 CACGTG CAC^GTG BciVI 2932 GGATAC GTATCC(6/5) BlpI 1554 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1554 GCTCAGC GC^T[ACGT]AGC BsaBI 633 GATTTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 4432 TCCGGT [AT]^CCGG[AT] BsiEI 4408 CGATCG CG[GA][CT]^CG Bsp1407I 3584 TGTACA T^GTACA BspLU11I 859 ACATGT A^CATGT BsrFI 669 ACCGGC [GA]^CCGG[CT] BsrGI 3584 TGTACA T^GTACA BssHII 200 GCGCGC G^CGCGC BssSI 4794 CACGAG CACGAG(-5/-1) BstAPI 4313 GCAGATCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CelII 1554 GCTCAGC GC^T[ACGT]AGC Cfr10I 669 ACCGGC [GA]^CCGG[CT] DraI 5493 TTTAAA TTT^AAA DraIII 2295 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 86 CGGCCA [CT]^GGCC[GA] Eam1105I 1484 GACGAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1867 AGCGCT AGC^GCT EcoRI 3378 GAATTC G^AATTC EcoRV 3578 GATATC GAT^ATC EcoT22I 4892 ATGCAT ATGCA^T HincII 5399 GTTAAC GT[CT]^[GA]AC HindII 5399 GTTAAC GT[CT]^[GA]AC HindIII 1951 AAGCTT A^AGCTT HpaI 5399 GTTAAC GTT^AAC KpnI 562 GGTACC GGTAC^C MamI 633 GATTTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1766 CAATTG C^AATTG MluI 1401 ACGCGT A^CGCGT MunI 1766 CAATTG C^AATTG NheI 4371 GCTAGC G^CTAGC NsiI 4892 ATGCAT ATGCA^T PaeR7I 748 CTCGAG C^TCGAG PflMI 4554 CCAAACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 1416 CACGTG CAC^GTG PmlI 1416 CACGTG CAC^GTG PvuI 4408 CGATCG CGAT^CG SgrAI 668 CACCGGCG C[GA]^CCGG[CT]G SmaI 221 CCCGGG CCC^GGG SspBI 3584 TGTACA T^GTACA Van91I 4554 CCAAACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 5420 CCAACAGGATAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 748 CTCGAG C^TCGAG XmaCI 221 CCCGGG C^CCGGG XmaI 221 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AcyI 178 GGCGTC G[GA]^CG[CT]C 4728 GGCGTC AflIII 859 ACATGT A^C[GA][CT]GT 1401 ACGCGT AvaI 221 CCCGGG C^[CT]CG[GA]G 748 CTCGAG AviII 2512 TGCGCA TGC^GCA 4285 TGCGCA BbvCI 1067 CCTCAGC CCTCAGC(-5/-2) 3186 GCTGAGG BglI 88 GCCAGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2256 GCCATGAAGGC BsaAI 1416 CACGTG [CT]AC^GT[GA] 2508 TACGTG BsaHI 178 GGCGTC G[GA]^CG[CT]C 4728 GGCGTC BsmI 2915 GAATGC GAATGC(1/-1) 5267 GCATTC BsoBI 221 CCCGGG C^[CT]CG[GA]G 748 CTCGAG BsrDI 514 GCAATG GCAATG(2/0) 1511 GCAATG BstBI 677 TTCGAA TT^CGAA 1826 TTCGAA DrdI 3735 GACTCTGCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4422 GACTGCAGAGTC EcoNI 570 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2780 CCTGGCAGAGG FspI 2512 TGCGCA TGC^GCA 4285 TGCGCA HaeII 394 AGCGCC [GA]GCGC^[CT] 1867 AGCGCT Hin1I 178 GGCGTC G[GA]^CG[CT]C 4728 GGCGTC KspI 2714 CCGCGG CCGC^GG 4151 CCGCGG NcoI 2455 CCATGG C^CATGG 4666 CCATGG NspV 677 TTCGAA TT^CGAA 1826 TTCGAA PshAI 73 GACTGCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 3396 GACCAGAGTC SacII 2714 CCGCGG CCGC^GG 4151 CCGCGG SapI 1326 GAAGAGC GCTCTTC(1/4) 3624 GCTCTTC ScaI 2872 AGTACT AGT^ACT 3589 AGTACT SfuI 677 TTCGAA TT^CGAA 1826 TTCGAA SspI 4527 AATATT AAT^ATT 5739 AATATT XbaI 1493 TCTAGA T^CTAGA 5243 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 114 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI ApaI ApoI AseI AsnI Asp700I AspHI AspI AvaII BanI BanII BbsI BbvI BcnI BfaI BglII BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrBI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflFI PleI PpuMI PshBI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XhoII XmnI