##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AgeI Alw44I AocI ApaLI AscI
AvrII BalI BamHI BbeI BcgI
BclI BfrI BlnI BseAI BsiWI
BsmBI BspDI BspEI BspHI Bst1107I
BstEII BstPI BstZ17I Bsu36I ClaI
CpoI EagI EclXI Eco52I Eco81I
EcoO65I FbaI FseI KasI MluNI
MroI MscI NaeI NarI NdeI
NgoMIV NotI NruI PacI PinAI
PmeI Psp1406I RcaI RsrII SalI
SbfI SexAI SfiI SfoI SnaBI
SpeI SphI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
Acc65I 562 GGTACC G^GTACC
AhdI 1484 GACGAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1867 AGCGCT AGC^GCT
Asp718I 562 GGTACC G^GTACC
AspEI 1484 GACGAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 1416 CACGTG CAC^GTG
BciVI 2932 GGATAC GTATCC(6/5)
BlpI 1554 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1554 GCTCAGC GC^T[ACGT]AGC
BsaBI 633 GATTTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 4432 TCCGGT [AT]^CCGG[AT]
BsiEI 4408 CGATCG CG[GA][CT]^CG
Bsp1407I 3584 TGTACA T^GTACA
BspLU11I 859 ACATGT A^CATGT
BsrFI 669 ACCGGC [GA]^CCGG[CT]
BsrGI 3584 TGTACA T^GTACA
BssHII 200 GCGCGC G^CGCGC
BssSI 4794 CACGAG CACGAG(-5/-1)
BstAPI 4313 GCAGATCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 1554 GCTCAGC GC^T[ACGT]AGC
Cfr10I 669 ACCGGC [GA]^CCGG[CT]
DraI 5493 TTTAAA TTT^AAA
DraIII 2295 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 86 CGGCCA [CT]^GGCC[GA]
Eam1105I 1484 GACGAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1867 AGCGCT AGC^GCT
EcoRI 3378 GAATTC G^AATTC
EcoRV 3578 GATATC GAT^ATC
EcoT22I 4892 ATGCAT ATGCA^T
HincII 5399 GTTAAC GT[CT]^[GA]AC
HindII 5399 GTTAAC GT[CT]^[GA]AC
HindIII 1951 AAGCTT A^AGCTT
HpaI 5399 GTTAAC GTT^AAC
KpnI 562 GGTACC GGTAC^C
MamI 633 GATTTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1766 CAATTG C^AATTG
MluI 1401 ACGCGT A^CGCGT
MunI 1766 CAATTG C^AATTG
NheI 4371 GCTAGC G^CTAGC
NsiI 4892 ATGCAT ATGCA^T
PaeR7I 748 CTCGAG C^TCGAG
PflMI 4554 CCAAACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1416 CACGTG CAC^GTG
PmlI 1416 CACGTG CAC^GTG
PvuI 4408 CGATCG CGAT^CG
SgrAI 668 CACCGGCG C[GA]^CCGG[CT]G
SmaI 221 CCCGGG CCC^GGG
SspBI 3584 TGTACA T^GTACA
Van91I 4554 CCAAACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 5420 CCAACAGGATAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 748 CTCGAG C^TCGAG
XmaCI 221 CCCGGG C^CCGGG
XmaI 221 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 178 GGCGTC G[GA]^CG[CT]C
4728 GGCGTC
AflIII 859 ACATGT A^C[GA][CT]GT
1401 ACGCGT
AvaI 221 CCCGGG C^[CT]CG[GA]G
748 CTCGAG
AviII 2512 TGCGCA TGC^GCA
4285 TGCGCA
BbvCI 1067 CCTCAGC CCTCAGC(-5/-2)
3186 GCTGAGG
BglI 88 GCCAGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2256 GCCATGAAGGC
BsaAI 1416 CACGTG [CT]AC^GT[GA]
2508 TACGTG
BsaHI 178 GGCGTC G[GA]^CG[CT]C
4728 GGCGTC
BsmI 2915 GAATGC GAATGC(1/-1)
5267 GCATTC
BsoBI 221 CCCGGG C^[CT]CG[GA]G
748 CTCGAG
BsrDI 514 GCAATG GCAATG(2/0)
1511 GCAATG
BstBI 677 TTCGAA TT^CGAA
1826 TTCGAA
DrdI 3735 GACTCTGCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4422 GACTGCAGAGTC
EcoNI 570 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2780 CCTGGCAGAGG
FspI 2512 TGCGCA TGC^GCA
4285 TGCGCA
HaeII 394 AGCGCC [GA]GCGC^[CT]
1867 AGCGCT
Hin1I 178 GGCGTC G[GA]^CG[CT]C
4728 GGCGTC
KspI 2714 CCGCGG CCGC^GG
4151 CCGCGG
NcoI 2455 CCATGG C^CATGG
4666 CCATGG
NspV 677 TTCGAA TT^CGAA
1826 TTCGAA
PshAI 73 GACTGCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3396 GACCAGAGTC
SacII 2714 CCGCGG CCGC^GG
4151 CCGCGG
SapI 1326 GAAGAGC GCTCTTC(1/4)
3624 GCTCTTC
ScaI 2872 AGTACT AGT^ACT
3589 AGTACT
SfuI 677 TTCGAA TT^CGAA
1826 TTCGAA
SspI 4527 AATATT AAT^ATT
5739 AATATT
XbaI 1493 TCTAGA T^CTAGA
5243 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApaI ApoI AseI
AsnI Asp700I AspHI AspI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BglII BmyI BpmI BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrBI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflFI PleI PpuMI
PshBI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I XhoII XmnI