##################################
# NO CUT ENZYMES ( 42 enzymes) #
##################################
AatII AclI AgeI Aor51HI AscI
BamHI BcgI BsaBI BsiWI BsmBI
BsrFI BssHII Bst1107I BstBI BstZ17I
Cfr10I CpoI Eco47III EcoRI EcoRV
FseI KspI MamI MluI NaeI
NgoMIV NruI NspV PaeR7I PflMI
PinAI PmeI Psp1406I RsrII SacII
SalI SexAI SfuI SgrAI SwaI
Van91I XhoI
###################################
# ONE CUT ENZYMES ( 64 enzymes) #
###################################
Acc65I 4685 GGTACC G^GTACC
AccII 218 CGCG CG^CG
AccIII 1424 TCCGGA T^CCGGA
AcyI 6337 GGCGCC G[GA]^CG[CT]C
AflII 2862 CTTAAG C^TTAAG
AhdI 296 GACCAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 866 CCTCAGG CC^T[ACGT]AGG
Asp700I 3261 GAACTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 4685 GGTACC G^GTACC
AspEI 296 GACCAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 6392 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 6190 TGGCCA TGG^CCA
BbeI 6337 GGCGCC GGCGC^C
BclI 4792 TGATCA T^GATCA
BfrI 2862 CTTAAG C^TTAAG
BglI 5055 GCCAACCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 6337 GGCGCC G[GA]^CG[CT]C
BseAI 1424 TCCGGA T^CCGGA
BspDI 4836 ATCGAT AT^CGAT
BspEI 1424 TCCGGA T^CCGGA
BspHI 4579 TCATGA T^CATGA
BsrBI 914 CCGCTC CCGCTC(-3/-3)
BssSI 1885 CTCGTG CACGAG(-5/-1)
BstEII 1575 GGTGACC G^GT[ACGT]ACC
BstPI 1575 GGTGACC G^GT[ACGT]ACC
BstUI 218 CGCG CG^CG
Bsu36I 866 CCTCAGG CC^T[ACGT]AGG
ClaI 4836 ATCGAT AT^CGAT
DrdI 4448 GACCTCGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 296 GACCAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 866 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1575 GGTGACC G^GT[ACGT]ACC
EcoT22I 1281 ATGCAT ATGCA^T
FbaI 4792 TGATCA T^GATCA
HaeII 6337 GGCGCC [GA]GCGC^[CT]
Hin1I 6337 GGCGCC G[GA]^CG[CT]C
HpaI 6537 GTTAAC GTT^AAC
KasI 6337 GGCGCC G^GCGCC
KpnI 4685 GGTACC GGTAC^C
MluNI 6190 TGGCCA TGG^CCA
MroI 1424 TCCGGA T^CCGGA
MscI 6190 TGGCCA TGG^CCA
MvnI 218 CGCG CG^CG
NarI 6337 GGCGCC GG^CGCC
NheI 6227 GCTAGC G^CTAGC
NotI 4954 GCGGCCGC GC^GGCCGC
NsiI 1281 ATGCAT ATGCA^T
PacI 5658 TTAATTAA TTAAT^TAA
PflFI 6392 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 3787 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 3457 GACCTTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 4555 CGATCG CGAT^CG
RcaI 4579 TCATGA T^CATGA
SfiI 5054 GGCCAACCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 6337 GGCGCC GGC^GCC
SmaI 2212 CCCGGG CCC^GGG
SnaBI 2175 TACGTA TAC^GTA
SpeI 5918 ACTAGT A^CTAGT
SspI 3135 AATATT AAT^ATT
Tth111I 6392 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1833 TCTAGA T^CTAGA
XmaCI 2212 CCCGGG C^CCGGG
XmaI 2212 CCCGGG C^CCGGG
XmnI 3261 GAACTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 938 GTAGAC GT^[AC][GT]AC
1379 GTAGAC
AseI 3132 ATTAAT AT^TAAT
6587 ATTAAT
AsnI 3132 ATTAAT AT^TAAT
6587 ATTAAT
AvrII 622 CCTAGG C^CTAGG
4326 CCTAGG
BbrPI 4563 CACGTG CAC^GTG
5073 CACGTG
BglII 593 AGATCT A^GATCT
985 AGATCT
BlnI 622 CCTAGG C^CTAGG
4326 CCTAGG
BstXI 1052 CCAGAGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5230 CCAATGAGGTGG
HincII 1038 GTCAAC GT[CT]^[GA]AC
6537 GTTAAC
HindII 1038 GTCAAC GT[CT]^[GA]AC
6537 GTTAAC
MfeI 3157 CAATTG C^AATTG
6505 CAATTG
MunI 3157 CAATTG C^AATTG
6505 CAATTG
PmaCI 4563 CACGTG CAC^GTG
5073 CACGTG
PmlI 4563 CACGTG CAC^GTG
5073 CACGTG
PshBI 3132 ATTAAT AT^TAAT
6587 ATTAAT
SbfI 257 CCTGCAGG CCTGCA^GG
3498 CCTGCAGG
Sse8387I 257 CCTGCAGG CCTGCA^GG
3498 CCTGCAGG
XcmI 3552 CCACAGCTGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
6604 CCAATAAGAAAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
Alw44I AlwI AlwNI ApaI ApaLI
ApoI AspHI AvaI AvaII AviII
BanI BanII BbsI BbvCI BbvI
BciVI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspLU11I
BspMI BsrDI BsrGI BsrI BssKI
BstAPI BstF5I BstNI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraI DraII DraIII DsaI EaeI
EagI EarI EclXI Eco52I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI FspI HaeIII HapII HgaI
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI SphI SspBI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII