##################################
# NO CUT ENZYMES ( 52 enzymes) #
##################################
AatII AclI AcsI AflII AgeI
Aor51HI ApoI AscI AseI AsnI
AviII AvrII BfrI BlnI BlpI
Bpu1102I BsiWI BspDI BspHI CelII
ClaI CpoI EagI EclXI Eco47III
Eco52I EcoRI FseI FspI HindIII
HpaI MfeI MluI MunI NheI
NotI NruI PacI PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SfiI SmaI SpeI SwaI XbaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 284 GGTACC G^GTACC
AccIII 331 TCCGGA T^CCGGA
Alw44I 3312 GTGCAC G^TGCAC
ApaLI 3312 GTGCAC G^TGCAC
Asp700I 2246 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 284 GGTACC G^GTACC
BbrPI 4580 CACGTG CAC^GTG
BcgI 3103 GCAAAGTGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3397 TGATCA T^GATCA
BsaBI 3887 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 331 TCCGGA [AT]^CCGG[AT]
BseAI 331 TCCGGA T^CCGGA
BsmBI 4852 CGTCTC CGTCTC(1/5)
BspEI 331 TCCGGA T^CCGGA
BssHII 142 GCGCGC G^CGCGC
BssSI 2370 CACGAG CACGAG(-5/-1)
Bst1107I 3026 GTATAC GTA^TAC
BstAPI 3256 GCATGTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 3986 CCAGCTAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 3026 GTATAC GTA^TAC
DraI 3153 TTTAAA TTT^AAA
DraIII 2869 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1856 GACCCCTAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 1890 GATATC GAT^ATC
EcoT22I 5104 ATGCAT ATGCA^T
FbaI 3397 TGATCA T^GATCA
KpnI 284 GGTACC GGTAC^C
MamI 3887 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 331 TCCGGA T^CCGGA
NaeI 4 GCCGGC GCC^GGC
NgoMIV 4 GCCGGC G^CCGGC
NsiI 5104 ATGCAT ATGCA^T
PaeR7I 865 CTCGAG C^TCGAG
PmaCI 4580 CACGTG CAC^GTG
PmlI 4580 CACGTG CAC^GTG
SbfI 1757 CCTGCAGG CCTGCA^GG
SgrAI 3 CGCCGGCG C[GA]^CCGG[CT]G
SnaBI 3852 TACGTA TAC^GTA
SphI 1747 GCATGC GCATG^C
Sse8387I 1757 CCTGCAGG CCTGCA^GG
SspI 586 AATATT AAT^ATT
XhoI 865 CTCGAG C^TCGAG
XmnI 2246 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AocI 2660 CCTGAGG CC^T[ACGT]AGG
3567 CCTGAGG
BamHI 3044 GGATCC G^GATCC
4771 GGATCC
BbvCI 447 CCTCAGC CCTCAGC(-5/-2)
3840 GCTGAGG
BsiEI 3452 CGACCG CG[GA][CT]^CG
4334 CGACCG
BsmI 4316 GCATTC GAATGC(1/-1)
5106 GCATTC
Bsp1407I 3468 TGTACA T^GTACA
4609 TGTACA
BspLU11I 1555 ACATGT A^CATGT
4149 ACATGT
BsrDI 3147 CATTGC GCAATG(2/0)
4214 CATTGC
BsrFI 4 GCCGGC [GA]^CCGG[CT]
3403 GCCGGT
BsrGI 3468 TGTACA T^GTACA
4609 TGTACA
BstBI 2253 TTCGAA TT^CGAA
4319 TTCGAA
Bsu36I 2660 CCTGAGG CC^T[ACGT]AGG
3567 CCTGAGG
Cfr10I 4 GCCGGC [GA]^CCGG[CT]
3403 GCCGGT
Eco81I 2660 CCTGAGG CC^T[ACGT]AGG
3567 CCTGAGG
KspI 97 CCGCGG CCGC^GG
173 CCGCGG
NcoI 1963 CCATGG C^CATGG
2932 CCATGG
NdeI 3458 CATATG CA^TATG
5029 CATATG
NspV 2253 TTCGAA TT^CGAA
4319 TTCGAA
PshAI 106 GACACCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2825 GACCAATGTC
SacII 97 CCGCGG CCGC^GG
173 CCGCGG
SalI 891 GTCGAC G^TCGAC
5093 GTCGAC
SapI 209 GAAGAGC GCTCTTC(1/4)
368 GAAGAGC
ScaI 562 AGTACT AGT^ACT
4410 AGTACT
SexAI 3202 ACCAGGT A^CC[AT]GGT
5140 ACCAGGT
SfuI 2253 TTCGAA TT^CGAA
4319 TTCGAA
SspBI 3468 TGTACA T^GTACA
4609 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AhdI AluI AlwI AlwNI
ApaI AspEI AspHI AspI AvaI
AvaII BalI BanI BanII BbeI
BbsI BbvI BciVI BcnI BfaI
BglI BglII BmyI BpmI BpuAI
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrI BssKI BstEII BstF5I BstNI
BstPI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI Eam1105I EarI EcoNI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NdeII
NlaIII NlaIV NspI PflFI PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XcmI XhoII