##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI Aor51HI AscI Asp718I AspEI
AviII BbeI BfrI BsaBI BsiWI
BsmBI Bsp1407I BspDI BspHI BsrBI
BsrGI BssHII BssSI Bst1107I BstZ17I
ClaI CpoI Eam1105I Eco47III EcoRV
EcoT22I FseI FspI KasI KpnI
MamI MfeI MunI NarI NheI
NotI NruI NsiI PacI PinAI
PmeI PshAI Psp1406I PvuI RcaI
RsrII SalI SbfI ScaI SfiI
SfoI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccIII 2402 TCCGGA T^CCGGA
AcyI 4796 GGCGTC G[GA]^CG[CT]C
Asp700I 214 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 463 GGATCC G^GATCC
BbrPI 1985 CACGTG CAC^GTG
BlpI 510 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 510 GCTGAGC GC^T[ACGT]AGC
BsaHI 4796 GGCGTC G[GA]^CG[CT]C
BseAI 2402 TCCGGA T^CCGGA
BsiEI 131 CGGCCG CG[GA][CT]^CG
BspEI 2402 TCCGGA T^CCGGA
BspLU11I 4829 ACATGT A^CATGT
BstAPI 1975 GCAGTCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1464 GGTGACC G^GT[ACGT]ACC
BstPI 1464 GGTGACC G^GT[ACGT]ACC
CelII 510 GCTGAGC GC^T[ACGT]AGC
DrdI 1969 GACGCTGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 131 CGGCCG C^GGCCG
EclXI 131 CGGCCG C^GGCCG
Eco52I 131 CGGCCG C^GGCCG
EcoO65I 1464 GGTGACC G^GT[ACGT]ACC
EcoRI 985 GAATTC G^AATTC
HaeII 3629 GGCGCT [GA]GCGC^[CT]
Hin1I 4796 GGCGTC G[GA]^CG[CT]C
HincII 2374 GTTAAC GT[CT]^[GA]AC
HindII 2374 GTTAAC GT[CT]^[GA]AC
HpaI 2374 GTTAAC GTT^AAC
KspI 80 CCGCGG CCGC^GG
MluI 634 ACGCGT A^CGCGT
MroI 2402 TCCGGA T^CCGGA
NaeI 1888 GCCGGC GCC^GGC
NdeI 4662 CATATG CA^TATG
NgoMIV 1888 GCCGGC G^CCGGC
PaeR7I 713 CTCGAG C^TCGAG
PmaCI 1985 CACGTG CAC^GTG
PmlI 1985 CACGTG CAC^GTG
SacII 80 CCGCGG CCGC^GG
SmaI 695 CCCGGG CCC^GGG
StuI 3275 AGGCCT AGG^CCT
XhoI 713 CTCGAG C^TCGAG
XmaCI 695 CCCGGG C^CCGGG
XmaI 695 CCCGGG C^CCGGG
XmnI 214 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 1033 GTAGAC GT^[AC][GT]AC
5096 GTCTAC
AccII 81 CGCG CG^CG
635 CGCG
Alw44I 1275 GTGCAC G^TGCAC
1982 GTGCAC
AocI 1207 CCTGAGG CC^T[ACGT]AGG
2223 CCTCAGG
ApaI 3023 GGGCCC GGGCC^C
3185 GGGCCC
ApaLI 1275 GTGCAC G^TGCAC
1982 GTGCAC
AseI 224 ATTAAT AT^TAAT
3403 ATTAAT
AsnI 224 ATTAAT AT^TAAT
3403 ATTAAT
AvrII 1450 CCTAGG C^CTAGG
4967 CCTAGG
BcgI 2673 CGACAGAGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2782 CGAGCCCTCTGC
BclI 1043 TGATCA T^GATCA
2498 TGATCA
BglI 1252 GCCAACAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1795 GCCCAGCGGGC
BglII 4761 AGATCT A^GATCT
5266 AGATCT
BlnI 1450 CCTAGG C^CTAGG
4967 CCTAGG
BsaAI 1537 TACGTG [CT]AC^GT[GA]
1985 CACGTG
BsaWI 1142 ACCGGA [AT]^CCGG[AT]
2402 TCCGGA
BstBI 249 TTCGAA TT^CGAA
2303 TTCGAA
BstUI 81 CGCG CG^CG
635 CGCG
Bsu36I 1207 CCTGAGG CC^T[ACGT]AGG
2223 CCTCAGG
DraI 3291 TTTAAA TTT^AAA
5313 TTTAAA
DraIII 1596 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
5237 CACATGGTG
Eco81I 1207 CCTGAGG CC^T[ACGT]AGG
2223 CCTCAGG
FbaI 1043 TGATCA T^GATCA
2498 TGATCA
HgaI 1969 GACGC GACGC(5/10)
4797 GCGTC
HindIII 3534 AAGCTT A^AGCTT
4864 AAGCTT
MvnI 81 CGCG CG^CG
635 CGCG
NspV 249 TTCGAA TT^CGAA
2303 TTCGAA
PshBI 224 ATTAAT AT^TAAT
3403 ATTAAT
SacI 1384 GAGCTC GAGCT^C
1475 GAGCTC
SapI 678 GCTCTTC GCTCTTC(1/4)
5201 GCTCTTC
SexAI 1931 ACCAGGT A^CC[AT]GGT
5091 ACCTGGT
SfuI 249 TTCGAA TT^CGAA
2303 TTCGAA
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AspHI AspI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BciVI BcnI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrDI BsrFI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflFI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XcmI XhoII