##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AatII AccIII AclI AgeI AscI
AviII AvrII BbeI BcgI BglI
BglII BlnI BsaBI BsaWI BseAI
BsiWI BspDI BspEI BssHII BstBI
BstEII BstPI ClaI CpoI EagI
EclXI Eco52I EcoO65I EcoRI FseI
FspI KasI KspI MamI MroI
NarI NheI NotI NruI NspV
PacI PinAI PmeI Psp1406I PvuI
RsrII SacII SbfI SfiI SfoI
SfuI SgrAI SnaBI Sse8387I StuI
XcmI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
Acc65I 1092 GGTACC G^GTACC
AflII 6613 CTTAAG C^TTAAG
AhdI 145 GACCTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1735 AGCGCT AGC^GCT
ApaI 2844 GGGCCC GGGCC^C
Asp718I 1092 GGTACC G^GTACC
AspEI 145 GACCTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 2353 GGATCC G^GATCC
BbrPI 1869 CACGTG CAC^GTG
BfrI 6613 CTTAAG C^TTAAG
BsaAI 1869 CACGTG [CT]AC^GT[GA]
BsiEI 674 CGACCG CG[GA][CT]^CG
BsrBI 6303 GAGCGG CCGCTC(-3/-3)
Bst1107I 4796 GTATAC GTA^TAC
BstXI 1258 CCACAGCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 4796 GTATAC GTA^TAC
DraIII 2411 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 145 GACCTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1735 AGCGCT AGC^GCT
EcoNI 1744 CCTCAGTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 4772 GATATC GAT^ATC
KpnI 1092 GGTACC GGTAC^C
MluI 3864 ACGCGT A^CGCGT
NaeI 1583 GCCGGC GCC^GGC
NgoMIV 1583 GCCGGC G^CCGGC
PaeR7I 1223 CTCGAG C^TCGAG
PmaCI 1869 CACGTG CAC^GTG
PmlI 1869 CACGTG CAC^GTG
PshAI 3980 GACATTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI 1391 ACCAGGT A^CC[AT]GGT
SmaI 6077 CCCGGG CCC^GGG
SpeI 6042 ACTAGT A^CTAGT
SphI 4354 GCATGC GCATG^C
SwaI 6642 ATTTAAAT ATTT^AAAT
XhoI 1223 CTCGAG C^TCGAG
XmaCI 6077 CCCGGG C^CCGGG
XmaI 6077 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Alw44I 4186 GTGCAC G^TGCAC
5430 GTGCAC
AlwNI 1243 CAGTCGCTG CAG[ACGT][ACGT][ACGT]^CTG
2593 CAGAGGCTG
ApaLI 4186 GTGCAC G^TGCAC
5430 GTGCAC
AspI 3750 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5339 GACAGTGTC
BalI 528 TGGCCA TGG^CCA
2771 TGGCCA
BclI 4738 TGATCA T^GATCA
4790 TGATCA
BsmBI 5949 CGTCTC CGTCTC(1/5)
4122 GAGACG
BspHI 4632 TCATGA T^CATGA
5818 TCATGA
BssSI 2421 CACGAG CACGAG(-5/-1)
3381 CTCGTG
EaeI 528 TGGCCA [CT]^GGCC[GA]
2771 TGGCCA
FbaI 4738 TGATCA T^GATCA
4790 TGATCA
HaeII 1436 AGCGCC [GA]GCGC^[CT]
1735 AGCGCT
HindIII 1570 AAGCTT A^AGCTT
1669 AAGCTT
HpaI 3059 GTTAAC GTT^AAC
4522 GTTAAC
MfeI 2078 CAATTG C^AATTG
3951 CAATTG
MluNI 528 TGGCCA TGG^CCA
2771 TGGCCA
MscI 528 TGGCCA TGG^CCA
2771 TGGCCA
MunI 2078 CAATTG C^AATTG
3951 CAATTG
PflFI 3750 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5339 GACAGTGTC
RcaI 4632 TCATGA T^CATGA
5818 TCATGA
SalI 608 GTCGAC G^TCGAC
1251 GTCGAC
ScaI 1302 AGTACT AGT^ACT
5238 AGTACT
Tth111I 3750 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5339 GACAGTGTC
XbaI 618 TCTAGA T^CTAGA
1494 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 141 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AocI
ApoI AseI AsnI Asp700I AspHI
AvaI AvaII BanI BanII BbsI
BbvCI BbvI BciVI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspLU11I
BspMI BsrDI BsrFI BsrGI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DrdI DsaI EarI Eco81I
EcoO109I EcoRII EcoT14I EcoT22I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeI NdeII NlaIII NlaIV
NsiI NspI PflMI PleI PpuMI
PshBI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII
XmnI