##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
Acc65I AclI AgeI AscI Asp718I
AspI AviII AvrII BamHI BbeI
BglI BlnI BsaBI BsiWI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
CpoI EagI EclXI Eco52I EcoO65I
FseI FspI HpaI KasI KpnI
MamI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI NspV
PaeR7I PflFI PinAI PshAI Psp1406I
PvuI RsrII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SnaBI
Sse8387I SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
AccI 6612 GTCTAC GT^[AC][GT]AC
AccII 2577 CGCG CG^CG
AccIII 5053 TCCGGA T^CCGGA
AflII 6289 CTTAAG C^TTAAG
BanI 53 GGTGCC G^G[CT][GA]CC
BfrI 6289 CTTAAG C^TTAAG
BseAI 5053 TCCGGA T^CCGGA
BsiEI 221 CGGTCG CG[GA][CT]^CG
BsmBI 3970 GAGACG CGTCTC(1/5)
BspDI 274 ATCGAT AT^CGAT
BspEI 5053 TCCGGA T^CCGGA
BsrFI 598 GCCGGT [GA]^CCGG[CT]
BstUI 2577 CGCG CG^CG
Cfr10I 598 GCCGGT [GA]^CCGG[CT]
ClaI 274 ATCGAT AT^CGAT
EcoNI 1299 CCTTCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HindIII 3440 AAGCTT A^AGCTT
KspI 2576 CCGCGG CCGC^GG
MfeI 5294 CAATTG C^AATTG
MroI 5053 TCCGGA T^CCGGA
MslI 3078 CATGACAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 5294 CAATTG C^AATTG
MvnI 2577 CGCG CG^CG
NcoI 1599 CCATGG C^CATGG
PacI 4981 TTAATTAA TTAAT^TAA
PmeI 5650 GTTTAAAC GTTT^AAAC
SacII 2576 CCGCGG CCGC^GG
XcmI 1599 CCATGGTTTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AatII 976 GACGTC GACGT^C
1276 GACGTC
AhdI 1271 GACAAGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3607 GACAAGCTGTC
Alw44I 296 GTGCAC G^TGCAC
4936 GTGCAC
ApaLI 296 GTGCAC G^TGCAC
4936 GTGCAC
AspEI 1271 GACAAGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3607 GACAAGCTGTC
BalI 2091 TGGCCA TGG^CCA
2489 TGGCCA
BbrPI 1421 CACGTG CAC^GTG
2293 CACGTG
BbvCI 2749 CCTCAGC CCTCAGC(-5/-2)
3006 GCTGAGG
BcgI 6629 CGAGTTGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
266 GCATCATCATCG
BciVI 1377 GGATAC GTATCC(6/5)
6132 GTATCC
BlpI 2958 GCTCAGC GC^T[ACGT]AGC
3170 GCTTAGC
Bpu1102I 2958 GCTCAGC GC^T[ACGT]AGC
3170 GCTTAGC
BsaAI 1421 CACGTG [CT]AC^GT[GA]
2293 CACGTG
BsaWI 999 TCCGGT [AT]^CCGG[AT]
5053 TCCGGA
BsmFI 1612 GGGAC GGGAC(10/14)
6196 GGGAC
BspLU11I 647 ACATGT A^CATGT
1936 ACATGT
BssSI 2896 CACGAG CACGAG(-5/-1)
4231 CACGAG
BstXI 2498 CCAGCCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3077 CCATGACAATGG
CelII 2958 GCTCAGC GC^T[ACGT]AGC
3170 GCTTAGC
DrdI 363 GACCCGTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6582 GACAGAGCAGTC
DsaI 1599 CCATGG C^C[GA][CT]GG
2576 CCGCGG
Eam1105I 1271 GACAAGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3607 GACAAGCTGTC
EcoRV 4585 GATATC GAT^ATC
6775 GATATC
EcoT22I 184 ATGCAT ATGCA^T
6046 ATGCAT
HincII 1030 GTCAAC GT[CT]^[GA]AC
5079 GTTGAC
HindII 1030 GTCAAC GT[CT]^[GA]AC
5079 GTTGAC
MluNI 2091 TGGCCA TGG^CCA
2489 TGGCCA
MscI 2091 TGGCCA TGG^CCA
2489 TGGCCA
NsiI 184 ATGCAT ATGCA^T
6046 ATGCAT
PmaCI 1421 CACGTG CAC^GTG
2293 CACGTG
PmlI 1421 CACGTG CAC^GTG
2293 CACGTG
PpuMI 191 AGGACCT [GA]G^G[AT]CC[CT]
6196 GGGACCC
ScaI 1909 AGTACT AGT^ACT
6692 AGTACT
SmaI 11 CCCGGG CCC^GGG
3740 CCCGGG
SphI 2136 GCATGC GCATG^C
3291 GCATGC
StuI 233 AGGCCT AGG^CCT
3020 AGGCCT
XmaCI 11 CCCGGG C^CCGGG
3740 CCCGGG
XmaI 11 CCCGGG C^CCGGG
3740 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwI AlwNI AocI Aor51HI
ApaI ApoI AseI AsnI Asp700I
AspHI AvaI AvaII BanII BbsI
BbvI BclI BcnI BfaI BglII
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmI BsoBI Bsp1286I
Bsp1407I BspHI BspMI BsrBI BsrDI
BsrGI BsrI BssKI BstAPI BstF5I
BstNI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DraIII EaeI EarI Eco47III Eco81I
EcoO109I EcoRI EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PshBI
PstI PvuII RcaI RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SpeI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmnI