################################## # NO CUT ENZYMES ( 60 enzymes) # ################################## Acc65I AclI AgeI AscI Asp718I AspI AviII AvrII BamHI BbeI BglI BlnI BsaBI BsiWI BssHII Bst1107I BstBI BstEII BstPI BstZ17I CpoI EagI EclXI Eco52I EcoO65I FseI FspI HpaI KasI KpnI MamI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PaeR7I PflFI PinAI PshAI Psp1406I PvuI RsrII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI Sse8387I SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 28 enzymes) # ################################### AccI 6612 GTCTAC GT^[AC][GT]AC AccII 2577 CGCG CG^CG AccIII 5053 TCCGGA T^CCGGA AflII 6289 CTTAAG C^TTAAG BanI 53 GGTGCC G^G[CT][GA]CC BfrI 6289 CTTAAG C^TTAAG BseAI 5053 TCCGGA T^CCGGA BsiEI 221 CGGTCG CG[GA][CT]^CG BsmBI 3970 GAGACG CGTCTC(1/5) BspDI 274 ATCGAT AT^CGAT BspEI 5053 TCCGGA T^CCGGA BsrFI 598 GCCGGT [GA]^CCGG[CT] BstUI 2577 CGCG CG^CG Cfr10I 598 GCCGGT [GA]^CCGG[CT] ClaI 274 ATCGAT AT^CGAT EcoNI 1299 CCTTCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HindIII 3440 AAGCTT A^AGCTT KspI 2576 CCGCGG CCGC^GG MfeI 5294 CAATTG C^AATTG MroI 5053 TCCGGA T^CCGGA MslI 3078 CATGACAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 5294 CAATTG C^AATTG MvnI 2577 CGCG CG^CG NcoI 1599 CCATGG C^CATGG PacI 4981 TTAATTAA TTAAT^TAA PmeI 5650 GTTTAAAC GTTT^AAAC SacII 2576 CCGCGG CCGC^GG XcmI 1599 CCATGGTTTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 38 enzymes) # ################################### AatII 976 GACGTC GACGT^C 1276 GACGTC AhdI 1271 GACAAGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3607 GACAAGCTGTC Alw44I 296 GTGCAC G^TGCAC 4936 GTGCAC ApaLI 296 GTGCAC G^TGCAC 4936 GTGCAC AspEI 1271 GACAAGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3607 GACAAGCTGTC BalI 2091 TGGCCA TGG^CCA 2489 TGGCCA BbrPI 1421 CACGTG CAC^GTG 2293 CACGTG BbvCI 2749 CCTCAGC CCTCAGC(-5/-2) 3006 GCTGAGG BcgI 6629 CGAGTTGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 266 GCATCATCATCG BciVI 1377 GGATAC GTATCC(6/5) 6132 GTATCC BlpI 2958 GCTCAGC GC^T[ACGT]AGC 3170 GCTTAGC Bpu1102I 2958 GCTCAGC GC^T[ACGT]AGC 3170 GCTTAGC BsaAI 1421 CACGTG [CT]AC^GT[GA] 2293 CACGTG BsaWI 999 TCCGGT [AT]^CCGG[AT] 5053 TCCGGA BsmFI 1612 GGGAC GGGAC(10/14) 6196 GGGAC BspLU11I 647 ACATGT A^CATGT 1936 ACATGT BssSI 2896 CACGAG CACGAG(-5/-1) 4231 CACGAG BstXI 2498 CCAGCCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3077 CCATGACAATGG CelII 2958 GCTCAGC GC^T[ACGT]AGC 3170 GCTTAGC DrdI 363 GACCCGTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 6582 GACAGAGCAGTC DsaI 1599 CCATGG C^C[GA][CT]GG 2576 CCGCGG Eam1105I 1271 GACAAGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3607 GACAAGCTGTC EcoRV 4585 GATATC GAT^ATC 6775 GATATC EcoT22I 184 ATGCAT ATGCA^T 6046 ATGCAT HincII 1030 GTCAAC GT[CT]^[GA]AC 5079 GTTGAC HindII 1030 GTCAAC GT[CT]^[GA]AC 5079 GTTGAC MluNI 2091 TGGCCA TGG^CCA 2489 TGGCCA MscI 2091 TGGCCA TGG^CCA 2489 TGGCCA NsiI 184 ATGCAT ATGCA^T 6046 ATGCAT PmaCI 1421 CACGTG CAC^GTG 2293 CACGTG PmlI 1421 CACGTG CAC^GTG 2293 CACGTG PpuMI 191 AGGACCT [GA]G^G[AT]CC[CT] 6196 GGGACCC ScaI 1909 AGTACT AGT^ACT 6692 AGTACT SmaI 11 CCCGGG CCC^GGG 3740 CCCGGG SphI 2136 GCATGC GCATG^C 3291 GCATGC StuI 233 AGGCCT AGG^CCT 3020 AGGCCT XmaCI 11 CCCGGG C^CCGGG 3740 CCCGGG XmaI 11 CCCGGG C^CCGGG 3740 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 132 enzymes) # ######################################### AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI AocI Aor51HI ApaI ApoI AseI AsnI Asp700I AspHI AvaI AvaII BanII BbsI BbvI BclI BcnI BfaI BglII BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmI BsoBI Bsp1286I Bsp1407I BspHI BspMI BsrBI BsrDI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DraIII EaeI EarI Eco47III Eco81I EcoO109I EcoRI EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PshBI PstI PvuII RcaI RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SpeI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI