################################## # NO CUT ENZYMES ( 90 enzymes) # ################################## AatII AccI AccIII AclI AcyI AflII AgeI Aor51HI AscI Asp700I BamHI BbeI BbrPI BbvCI BcgI BclI BfrI BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BssHII Bst1107I BstZ17I Cfr10I ClaI CpoI DraI DraIII DrdI EagI EclXI Eco47III Eco52I EcoRI EcoT22I FbaI FseI HaeII Hin1I HincII HindII HpaI KasI KspI MamI MfeI MroI MunI NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacI SacII SalI SfiI SfoI SgrAI SmaI SnaBI SphI SspI SwaI XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccII 3300 CGCG CG^CG AcsI 3176 GAATTT [GA]^AATT[CT] AflIII 3299 ACGCGT A^C[GA][CT]GT AhdI 2067 GACACCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 2773 GTGCAC G^TGCAC AocI 3071 CCTGAGG CC^T[ACGT]AGG ApaLI 2773 GTGCAC G^TGCAC ApoI 3176 GAATTT [GA]^AATT[CT] AspEI 2067 GACACCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 2352 TGCGCA TGC^GCA AvrII 3688 CCTAGG C^CTAGG BglII 3419 AGATCT A^GATCT BlnI 3688 CCTAGG C^CTAGG BsaI 4397 GAGACC GGTCTC(1/5) BsmBI 3948 GAGACG CGTCTC(1/5) Bsp1407I 3666 TGTACA T^GTACA BspHI 2177 TCATGA T^CATGA BsrGI 3666 TGTACA T^GTACA BssSI 4141 CTCGTG CACGAG(-5/-1) BstUI 3300 CGCG CG^CG Bsu36I 3071 CCTGAGG CC^T[ACGT]AGG CfoI 2353 GCGC GCG^C Eam1105I 2067 GACACCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 3071 CCTGAGG CC^T[ACGT]AGG EcoRV 2439 GATATC GAT^ATC FspI 2352 TGCGCA TGC^GCA HgaI 3301 GCGTC GACGC(5/10) HhaI 2353 GCGC GCG^C HinP1I 2353 GCGC G^CGC MluI 3299 ACGCGT A^CGCGT MvnI 3300 CGCG CG^CG NdeI 1971 CATATG CA^TATG PshAI 68 GACCAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 2177 TCATGA T^CATGA SapI 3360 GCTCTTC GCTCTTC(1/4) SbfI 2824 CCTGCAGG CCTGCA^GG SpeI 3849 ACTAGT A^CTAGT Sse8387I 2824 CCTGCAGG CCTGCA^GG SspBI 3666 TGTACA T^GTACA TfiI 4409 GAATC G^A[AT]TC ################################### # TWO CUT ENZYMES ( 37 enzymes) # ################################### Acc65I 1278 GGTACC G^GTACC 1719 GGTACC AlwI 3162 GATCC GGATC(4/5) 3220 GATCC ApaI 2496 GGGCCC GGGCC^C 3351 GGGCCC AseI 1560 ATTAAT AT^TAAT 1659 ATTAAT AsnI 1560 ATTAAT AT^TAAT 1659 ATTAAT Asp718I 1278 GGTACC G^GTACC 1719 GGTACC AspI 1992 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC 3433 GACTATGTC AvaI 209 CTCGGG C^[CT]CG[GA]G 2500 CCCGAG BcnI 142 CCCGG CC^[GC]GG 2519 CCGGG BlpI 2472 GCTCAGC GC^T[ACGT]AGC 4043 GCTAAGC Bpu1102I 2472 GCTCAGC GC^T[ACGT]AGC 4043 GCTAAGC BseRI 3734 CTCCTC GAGGAG(10/8) 2373 GAGGAG BsmI 3459 GAATGC GAATGC(1/-1) 3935 GAATGC BsoBI 209 CTCGGG C^[CT]CG[GA]G 2500 CCCGAG BstAPI 258 GCAGGGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2827 GCAGGGCCTGC BstBI 1898 TTCGAA TT^CGAA 2577 TTCGAA BstYI 3161 AGATCC [GA]^GATC[CT] 3419 AGATCT CelII 2472 GCTCAGC GC^T[ACGT]AGC 4043 GCTAAGC EarI 3361 CTCTTC CTCTTC(1/4) 4164 GAAGAG FauI 2570 GCGGG CCCGC(4/6) 2746 GCGGG HapII 143 CCGG C^CGG 2519 CCGG HindIII 3904 AAGCTT A^AGCTT 4046 AAGCTT HpaII 143 CCGG C^CGG 2519 CCGG KpnI 1278 GGTACC GGTAC^C 1719 GGTACC Ksp632I 3361 CTCTTC CTCTTC(1/4) 4164 GAAGAG MflI 3161 AGATCC [GA]^GATC[CT] 3419 AGATCT MspI 143 CCGG C^CGG 2519 CCGG NciI 142 CCCGG CC^[GC]GG 2519 CCGGG NspV 1898 TTCGAA TT^CGAA 2577 TTCGAA PflFI 1992 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC 3433 GACTATGTC PshBI 1560 ATTAAT AT^TAAT 1659 ATTAAT ScaI 2504 AGTACT AGT^ACT 3846 AGTACT SexAI 148 ACCTGGT A^CC[AT]GGT 2361 ACCAGGT SfuI 1898 TTCGAA TT^CGAA 2577 TTCGAA Tsp509I 3177 AATT ^AATT 3538 AATT Tth111I 1992 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC 3433 GACTATGTC XhoII 3161 AGATCC [GA]^GATC[CT] 3419 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 91 enzymes) # ######################################### AciI AfaI AluI AlwNI AspHI AvaII BalI BanI BanII BbsI BbvI BciVI BfaI BglI BmyI BpmI BpuAI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstEII BstF5I BstNI BstPI BstXI Cac8I Cfr13I DdeI DpnII DraII DsaI EaeI EcoNI EcoO109I EcoO65I EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MvaI MwoI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI Tru9I TseI Tsp45I TspRI TthHB8I Van91I XcmI