##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AflII AgeI Aor51HI AscI Asp700I
BamHI BbeI BbrPI BbvCI BcgI
BclI BfrI BsaAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI BspDI
BspEI BspLU11I BsrBI BsrDI BsrFI
BssHII Bst1107I BstZ17I Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
EclXI Eco47III Eco52I EcoRI EcoT22I
FbaI FseI HaeII Hin1I HincII
HindII HpaI KasI KspI MamI
MfeI MroI MunI NaeI NarI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PinAI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SacI
SacII SalI SfiI SfoI SgrAI
SmaI SnaBI SphI SspI SwaI
XbaI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccII 3300 CGCG CG^CG
AcsI 3176 GAATTT [GA]^AATT[CT]
AflIII 3299 ACGCGT A^C[GA][CT]GT
AhdI 2067 GACACCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2773 GTGCAC G^TGCAC
AocI 3071 CCTGAGG CC^T[ACGT]AGG
ApaLI 2773 GTGCAC G^TGCAC
ApoI 3176 GAATTT [GA]^AATT[CT]
AspEI 2067 GACACCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2352 TGCGCA TGC^GCA
AvrII 3688 CCTAGG C^CTAGG
BglII 3419 AGATCT A^GATCT
BlnI 3688 CCTAGG C^CTAGG
BsaI 4397 GAGACC GGTCTC(1/5)
BsmBI 3948 GAGACG CGTCTC(1/5)
Bsp1407I 3666 TGTACA T^GTACA
BspHI 2177 TCATGA T^CATGA
BsrGI 3666 TGTACA T^GTACA
BssSI 4141 CTCGTG CACGAG(-5/-1)
BstUI 3300 CGCG CG^CG
Bsu36I 3071 CCTGAGG CC^T[ACGT]AGG
CfoI 2353 GCGC GCG^C
Eam1105I 2067 GACACCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3071 CCTGAGG CC^T[ACGT]AGG
EcoRV 2439 GATATC GAT^ATC
FspI 2352 TGCGCA TGC^GCA
HgaI 3301 GCGTC GACGC(5/10)
HhaI 2353 GCGC GCG^C
HinP1I 2353 GCGC G^CGC
MluI 3299 ACGCGT A^CGCGT
MvnI 3300 CGCG CG^CG
NdeI 1971 CATATG CA^TATG
PshAI 68 GACCAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2177 TCATGA T^CATGA
SapI 3360 GCTCTTC GCTCTTC(1/4)
SbfI 2824 CCTGCAGG CCTGCA^GG
SpeI 3849 ACTAGT A^CTAGT
Sse8387I 2824 CCTGCAGG CCTGCA^GG
SspBI 3666 TGTACA T^GTACA
TfiI 4409 GAATC G^A[AT]TC
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
Acc65I 1278 GGTACC G^GTACC
1719 GGTACC
AlwI 3162 GATCC GGATC(4/5)
3220 GATCC
ApaI 2496 GGGCCC GGGCC^C
3351 GGGCCC
AseI 1560 ATTAAT AT^TAAT
1659 ATTAAT
AsnI 1560 ATTAAT AT^TAAT
1659 ATTAAT
Asp718I 1278 GGTACC G^GTACC
1719 GGTACC
AspI 1992 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3433 GACTATGTC
AvaI 209 CTCGGG C^[CT]CG[GA]G
2500 CCCGAG
BcnI 142 CCCGG CC^[GC]GG
2519 CCGGG
BlpI 2472 GCTCAGC GC^T[ACGT]AGC
4043 GCTAAGC
Bpu1102I 2472 GCTCAGC GC^T[ACGT]AGC
4043 GCTAAGC
BseRI 3734 CTCCTC GAGGAG(10/8)
2373 GAGGAG
BsmI 3459 GAATGC GAATGC(1/-1)
3935 GAATGC
BsoBI 209 CTCGGG C^[CT]CG[GA]G
2500 CCCGAG
BstAPI 258 GCAGGGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2827 GCAGGGCCTGC
BstBI 1898 TTCGAA TT^CGAA
2577 TTCGAA
BstYI 3161 AGATCC [GA]^GATC[CT]
3419 AGATCT
CelII 2472 GCTCAGC GC^T[ACGT]AGC
4043 GCTAAGC
EarI 3361 CTCTTC CTCTTC(1/4)
4164 GAAGAG
FauI 2570 GCGGG CCCGC(4/6)
2746 GCGGG
HapII 143 CCGG C^CGG
2519 CCGG
HindIII 3904 AAGCTT A^AGCTT
4046 AAGCTT
HpaII 143 CCGG C^CGG
2519 CCGG
KpnI 1278 GGTACC GGTAC^C
1719 GGTACC
Ksp632I 3361 CTCTTC CTCTTC(1/4)
4164 GAAGAG
MflI 3161 AGATCC [GA]^GATC[CT]
3419 AGATCT
MspI 143 CCGG C^CGG
2519 CCGG
NciI 142 CCCGG CC^[GC]GG
2519 CCGGG
NspV 1898 TTCGAA TT^CGAA
2577 TTCGAA
PflFI 1992 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3433 GACTATGTC
PshBI 1560 ATTAAT AT^TAAT
1659 ATTAAT
ScaI 2504 AGTACT AGT^ACT
3846 AGTACT
SexAI 148 ACCTGGT A^CC[AT]GGT
2361 ACCAGGT
SfuI 1898 TTCGAA TT^CGAA
2577 TTCGAA
Tsp509I 3177 AATT ^AATT
3538 AATT
Tth111I 1992 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3433 GACTATGTC
XhoII 3161 AGATCC [GA]^GATC[CT]
3419 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AciI AfaI AluI AlwNI AspHI
AvaII BalI BanI BanII BbsI
BbvI BciVI BfaI BglI BmyI
BpmI BpuAI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstEII BstF5I
BstNI BstPI BstXI Cac8I Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MspA1I MvaI MwoI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI Tru9I
TseI Tsp45I TspRI TthHB8I Van91I
XcmI