##################################
# NO CUT ENZYMES (   46 enzymes) #
##################################

AccIII      AflII       AgeI        AscI        AviII       
BbrPI       BcgI        BclI        BfrI        BsaBI       
BseAI       BsiWI       BspDI       BspEI       BspHI       
BstBI       ClaI        CpoI        EcoRV       FbaI        
FseI        FspI        MamI        MluI        MroI        
NotI        NruI        NspV        PacI        PinAI       
PmaCI       PmeI        PmlI        PvuI        RcaI        
RsrII       SbfI        SexAI       SfiI        SfuI        
SgrAI       SnaBI       SpeI        Sse8387I    SspI        
SwaI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

AatII        4994  GACGTC               GACGT^C
Acc65I       5407  GGTACC               G^GTACC
AclI            9  AACGTT               AA^CGTT
Aor51HI       620  AGCGCT               AGC^GCT
AseI         4174  ATTAAT               AT^TAAT
AsnI         4174  ATTAAT               AT^TAAT
Asp700I      6014  GAAAAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      5407  GGTACC               G^GTACC
AspI         3324  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII        2393  CCTAGG               C^CTAGG
BalI         3674  TGGCCA               TGG^CCA
BamHI        2669  GGATCC               G^GATCC
BbeI         1363  GGCGCC               GGCGC^C
BglII        4138  AGATCT               A^GATCT
BlnI         2393  CCTAGG               C^CTAGG
BsaAI        5038  CACGTA               [CT]AC^GT[GA]
BsaWI        2580  ACCGGA               [AT]^CCGG[AT]
BsmBI        5968  GAGACG               CGTCTC(1/5)
BsrFI        4666  GCCGGC               [GA]^CCGG[CT]
BssSI         482  CTCGTG               CACGAG(-5/-1)
Bst1107I      612  GTATAC               GTA^TAC
BstEII        917  GGTCACC              G^GT[ACGT]ACC
BstPI         917  GGTCACC              G^GT[ACGT]ACC
BstXI        4811  CCACAACACTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I       612  GTATAC               GTA^TAC
Cfr10I       4666  GCCGGC               [GA]^CCGG[CT]
DraIII       1740  CACTGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1221  GACCTTTCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      620  AGCGCT               AGC^GCT
EcoO65I       917  GGTCACC              G^GT[ACGT]ACC
HpaI         4822  GTTAAC               GTT^AAC
KasI         1363  GGCGCC               G^GCGCC
KpnI         5407  GGTACC               GGTAC^C
KspI          252  CCGCGG               CCGC^GG
MfeI         3467  CAATTG               C^AATTG
MluNI        3674  TGGCCA               TGG^CCA
MscI         3674  TGGCCA               TGG^CCA
MunI         3467  CAATTG               C^AATTG
NaeI         4666  GCCGGC               GCC^GGC
NarI         1363  GGCGCC               GG^CGCC
NdeI         3301  CATATG               CA^TATG
NgoMIV       4666  GCCGGC               G^CCGGC
NheI          306  GCTAGC               G^CTAGC
PflFI        3324  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI        2547  GACAGTTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        4174  ATTAAT               AT^TAAT
Psp1406I        9  AACGTT               AA^CGTT
SacI         1117  GAGCTC               GAGCT^C
SacII         252  CCGCGG               CCGC^GG
SalI          128  GTCGAC               G^TCGAC
SapI         1356  GAAGAGC              GCTCTTC(1/4)
SfoI         1363  GGCGCC               GGC^GCC
StuI         4299  AGGCCT               AGG^CCT
Tth111I      3324  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         6304  TCTAGA               T^CTAGA
XmnI         6014  GAAAAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AcyI         1363  GGCGCC               G[GA]^CG[CT]C
             4994  GACGTC              
AhdI          558  GACCCAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3021  GACAGCACGTC         
AspEI         558  GACCCAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3021  GACAGCACGTC         
BbsI         1508  GAAGAC               GAAGAC(2/6)
             1539  GAAGAC              
BciVI        2563  GTATCC               GTATCC(6/5)
             3386  GTATCC              
BpuAI        1508  GAAGAC               GAAGAC(2/6)
             1539  GAAGAC              
BsaHI        1363  GGCGCC               G[GA]^CG[CT]C
             4994  GACGTC              
BsaI         2650  GGTCTC               GGTCTC(1/5)
             4649  GGTCTC              
BsmI         3056  GAATGC               GAATGC(1/-1)
             5682  GCATTC              
Bsp1407I     3962  TGTACA               T^GTACA
             6210  TGTACA              
BspMI         520  ACCTGC               ACCTGC(4/8)
             5410  ACCTGC              
BsrGI        3962  TGTACA               T^GTACA
             6210  TGTACA              
BssHII        501  GCGCGC               G^CGCGC
              704  GCGCGC              
BstAPI        802  GCAGTGGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             3775  GCACAGCATGC         
DraI         4781  TTTAAA               TTT^AAA
             5563  TTTAAA              
EagI           20  CGGCCG               C^GGCCG
              183  CGGCCG              
Eam1105I      558  GACCCAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3021  GACAGCACGTC         
EclXI          20  CGGCCG               C^GGCCG
              183  CGGCCG              
Eco52I         20  CGGCCG               C^GGCCG
              183  CGGCCG              
EcoT22I      3782  ATGCAT               ATGCA^T
             5045  ATGCAT              
Hin1I        1363  GGCGCC               G[GA]^CG[CT]C
             4994  GACGTC              
NsiI         3782  ATGCAT               ATGCA^T
             5045  ATGCAT              
PaeR7I        291  CTCGAG               C^TCGAG
              712  CTCGAG              
PpuMI         257  GGGACCC              [GA]G^G[AT]CC[CT]
             3092  GGGTCCC             
SmaI           34  CCCGGG               CCC^GGG
              283  CCCGGG              
SspBI        3962  TGTACA               T^GTACA
             6210  TGTACA              
XhoI          291  CTCGAG               C^TCGAG
              712  CTCGAG              
XmaCI          34  CCCGGG               C^CCGGG
              283  CCCGGG              
XmaI           34  CCCGGG               C^CCGGG
              283  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  127 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AfaI        
AflIII      AluI        Alw44I      AlwI        AlwNI       
AocI        ApaI        ApaLI       ApoI        AspHI       
AvaI        AvaII       BanI        BanII       BbvCI       
BbvI        BcnI        BfaI        BglI        BlpI        
BmyI        BpmI        Bpu1102I    BsaJI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BspLU11I    
BsrBI       BsrDI       BsrI        BssKI       BstF5I      
BstNI       BstUI       BstYI       Bsu36I      Cac8I       
CelII       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        Eco81I      
EcoNI       EcoO109I    EcoRI       EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        HinP1I      HincII      
HindII      HindIII     HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NcoI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PleI        PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScaI        
ScrFI       SfaNI       SfcI        SmlI        SphI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XcmI        XhoII