##################################
# NO CUT ENZYMES ( 46 enzymes) #
##################################
AccIII AflII AgeI AscI AviII
BbrPI BcgI BclI BfrI BsaBI
BseAI BsiWI BspDI BspEI BspHI
BstBI ClaI CpoI EcoRV FbaI
FseI FspI MamI MluI MroI
NotI NruI NspV PacI PinAI
PmaCI PmeI PmlI PvuI RcaI
RsrII SbfI SexAI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
SwaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AatII 4994 GACGTC GACGT^C
Acc65I 5407 GGTACC G^GTACC
AclI 9 AACGTT AA^CGTT
Aor51HI 620 AGCGCT AGC^GCT
AseI 4174 ATTAAT AT^TAAT
AsnI 4174 ATTAAT AT^TAAT
Asp700I 6014 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 5407 GGTACC G^GTACC
AspI 3324 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2393 CCTAGG C^CTAGG
BalI 3674 TGGCCA TGG^CCA
BamHI 2669 GGATCC G^GATCC
BbeI 1363 GGCGCC GGCGC^C
BglII 4138 AGATCT A^GATCT
BlnI 2393 CCTAGG C^CTAGG
BsaAI 5038 CACGTA [CT]AC^GT[GA]
BsaWI 2580 ACCGGA [AT]^CCGG[AT]
BsmBI 5968 GAGACG CGTCTC(1/5)
BsrFI 4666 GCCGGC [GA]^CCGG[CT]
BssSI 482 CTCGTG CACGAG(-5/-1)
Bst1107I 612 GTATAC GTA^TAC
BstEII 917 GGTCACC G^GT[ACGT]ACC
BstPI 917 GGTCACC G^GT[ACGT]ACC
BstXI 4811 CCACAACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 612 GTATAC GTA^TAC
Cfr10I 4666 GCCGGC [GA]^CCGG[CT]
DraIII 1740 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1221 GACCTTTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 620 AGCGCT AGC^GCT
EcoO65I 917 GGTCACC G^GT[ACGT]ACC
HpaI 4822 GTTAAC GTT^AAC
KasI 1363 GGCGCC G^GCGCC
KpnI 5407 GGTACC GGTAC^C
KspI 252 CCGCGG CCGC^GG
MfeI 3467 CAATTG C^AATTG
MluNI 3674 TGGCCA TGG^CCA
MscI 3674 TGGCCA TGG^CCA
MunI 3467 CAATTG C^AATTG
NaeI 4666 GCCGGC GCC^GGC
NarI 1363 GGCGCC GG^CGCC
NdeI 3301 CATATG CA^TATG
NgoMIV 4666 GCCGGC G^CCGGC
NheI 306 GCTAGC G^CTAGC
PflFI 3324 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 2547 GACAGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4174 ATTAAT AT^TAAT
Psp1406I 9 AACGTT AA^CGTT
SacI 1117 GAGCTC GAGCT^C
SacII 252 CCGCGG CCGC^GG
SalI 128 GTCGAC G^TCGAC
SapI 1356 GAAGAGC GCTCTTC(1/4)
SfoI 1363 GGCGCC GGC^GCC
StuI 4299 AGGCCT AGG^CCT
Tth111I 3324 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 6304 TCTAGA T^CTAGA
XmnI 6014 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 1363 GGCGCC G[GA]^CG[CT]C
4994 GACGTC
AhdI 558 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3021 GACAGCACGTC
AspEI 558 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3021 GACAGCACGTC
BbsI 1508 GAAGAC GAAGAC(2/6)
1539 GAAGAC
BciVI 2563 GTATCC GTATCC(6/5)
3386 GTATCC
BpuAI 1508 GAAGAC GAAGAC(2/6)
1539 GAAGAC
BsaHI 1363 GGCGCC G[GA]^CG[CT]C
4994 GACGTC
BsaI 2650 GGTCTC GGTCTC(1/5)
4649 GGTCTC
BsmI 3056 GAATGC GAATGC(1/-1)
5682 GCATTC
Bsp1407I 3962 TGTACA T^GTACA
6210 TGTACA
BspMI 520 ACCTGC ACCTGC(4/8)
5410 ACCTGC
BsrGI 3962 TGTACA T^GTACA
6210 TGTACA
BssHII 501 GCGCGC G^CGCGC
704 GCGCGC
BstAPI 802 GCAGTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3775 GCACAGCATGC
DraI 4781 TTTAAA TTT^AAA
5563 TTTAAA
EagI 20 CGGCCG C^GGCCG
183 CGGCCG
Eam1105I 558 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3021 GACAGCACGTC
EclXI 20 CGGCCG C^GGCCG
183 CGGCCG
Eco52I 20 CGGCCG C^GGCCG
183 CGGCCG
EcoT22I 3782 ATGCAT ATGCA^T
5045 ATGCAT
Hin1I 1363 GGCGCC G[GA]^CG[CT]C
4994 GACGTC
NsiI 3782 ATGCAT ATGCA^T
5045 ATGCAT
PaeR7I 291 CTCGAG C^TCGAG
712 CTCGAG
PpuMI 257 GGGACCC [GA]G^G[AT]CC[CT]
3092 GGGTCCC
SmaI 34 CCCGGG CCC^GGG
283 CCCGGG
SspBI 3962 TGTACA T^GTACA
6210 TGTACA
XhoI 291 CTCGAG C^TCGAG
712 CTCGAG
XmaCI 34 CCCGGG C^CCGGG
283 CCCGGG
XmaI 34 CCCGGG C^CCGGG
283 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI Alw44I AlwI AlwNI
AocI ApaI ApaLI ApoI AspHI
AvaI AvaII BanI BanII BbvCI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspLU11I
BsrBI BsrDI BsrI BssKI BstF5I
BstNI BstUI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRI EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PstI
PvuII RsaI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI SphI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII