################################## # NO CUT ENZYMES ( 46 enzymes) # ################################## AccIII AflII AgeI AscI AviII BbrPI BcgI BclI BfrI BsaBI BseAI BsiWI BspDI BspEI BspHI BstBI ClaI CpoI EcoRV FbaI FseI FspI MamI MluI MroI NotI NruI NspV PacI PinAI PmaCI PmeI PmlI PvuI RcaI RsrII SbfI SexAI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspI SwaI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AatII 4994 GACGTC GACGT^C Acc65I 5407 GGTACC G^GTACC AclI 9 AACGTT AA^CGTT Aor51HI 620 AGCGCT AGC^GCT AseI 4174 ATTAAT AT^TAAT AsnI 4174 ATTAAT AT^TAAT Asp700I 6014 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 5407 GGTACC G^GTACC AspI 3324 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 2393 CCTAGG C^CTAGG BalI 3674 TGGCCA TGG^CCA BamHI 2669 GGATCC G^GATCC BbeI 1363 GGCGCC GGCGC^C BglII 4138 AGATCT A^GATCT BlnI 2393 CCTAGG C^CTAGG BsaAI 5038 CACGTA [CT]AC^GT[GA] BsaWI 2580 ACCGGA [AT]^CCGG[AT] BsmBI 5968 GAGACG CGTCTC(1/5) BsrFI 4666 GCCGGC [GA]^CCGG[CT] BssSI 482 CTCGTG CACGAG(-5/-1) Bst1107I 612 GTATAC GTA^TAC BstEII 917 GGTCACC G^GT[ACGT]ACC BstPI 917 GGTCACC G^GT[ACGT]ACC BstXI 4811 CCACAACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 612 GTATAC GTA^TAC Cfr10I 4666 GCCGGC [GA]^CCGG[CT] DraIII 1740 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1221 GACCTTTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 620 AGCGCT AGC^GCT EcoO65I 917 GGTCACC G^GT[ACGT]ACC HpaI 4822 GTTAAC GTT^AAC KasI 1363 GGCGCC G^GCGCC KpnI 5407 GGTACC GGTAC^C KspI 252 CCGCGG CCGC^GG MfeI 3467 CAATTG C^AATTG MluNI 3674 TGGCCA TGG^CCA MscI 3674 TGGCCA TGG^CCA MunI 3467 CAATTG C^AATTG NaeI 4666 GCCGGC GCC^GGC NarI 1363 GGCGCC GG^CGCC NdeI 3301 CATATG CA^TATG NgoMIV 4666 GCCGGC G^CCGGC NheI 306 GCTAGC G^CTAGC PflFI 3324 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 2547 GACAGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 4174 ATTAAT AT^TAAT Psp1406I 9 AACGTT AA^CGTT SacI 1117 GAGCTC GAGCT^C SacII 252 CCGCGG CCGC^GG SalI 128 GTCGAC G^TCGAC SapI 1356 GAAGAGC GCTCTTC(1/4) SfoI 1363 GGCGCC GGC^GCC StuI 4299 AGGCCT AGG^CCT Tth111I 3324 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 6304 TCTAGA T^CTAGA XmnI 6014 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AcyI 1363 GGCGCC G[GA]^CG[CT]C 4994 GACGTC AhdI 558 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3021 GACAGCACGTC AspEI 558 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3021 GACAGCACGTC BbsI 1508 GAAGAC GAAGAC(2/6) 1539 GAAGAC BciVI 2563 GTATCC GTATCC(6/5) 3386 GTATCC BpuAI 1508 GAAGAC GAAGAC(2/6) 1539 GAAGAC BsaHI 1363 GGCGCC G[GA]^CG[CT]C 4994 GACGTC BsaI 2650 GGTCTC GGTCTC(1/5) 4649 GGTCTC BsmI 3056 GAATGC GAATGC(1/-1) 5682 GCATTC Bsp1407I 3962 TGTACA T^GTACA 6210 TGTACA BspMI 520 ACCTGC ACCTGC(4/8) 5410 ACCTGC BsrGI 3962 TGTACA T^GTACA 6210 TGTACA BssHII 501 GCGCGC G^CGCGC 704 GCGCGC BstAPI 802 GCAGTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3775 GCACAGCATGC DraI 4781 TTTAAA TTT^AAA 5563 TTTAAA EagI 20 CGGCCG C^GGCCG 183 CGGCCG Eam1105I 558 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3021 GACAGCACGTC EclXI 20 CGGCCG C^GGCCG 183 CGGCCG Eco52I 20 CGGCCG C^GGCCG 183 CGGCCG EcoT22I 3782 ATGCAT ATGCA^T 5045 ATGCAT Hin1I 1363 GGCGCC G[GA]^CG[CT]C 4994 GACGTC NsiI 3782 ATGCAT ATGCA^T 5045 ATGCAT PaeR7I 291 CTCGAG C^TCGAG 712 CTCGAG PpuMI 257 GGGACCC [GA]G^G[AT]CC[CT] 3092 GGGTCCC SmaI 34 CCCGGG CCC^GGG 283 CCCGGG SspBI 3962 TGTACA T^GTACA 6210 TGTACA XhoI 291 CTCGAG C^TCGAG 712 CTCGAG XmaCI 34 CCCGGG C^CCGGG 283 CCCGGG XmaI 34 CCCGGG C^CCGGG 283 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 127 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AflIII AluI Alw44I AlwI AlwNI AocI ApaI ApaLI ApoI AspHI AvaI AvaII BanI BanII BbvCI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspLU11I BsrBI BsrDI BsrI BssKI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PstI PvuII RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SphI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII