################################## # NO CUT ENZYMES ( 53 enzymes) # ################################## AatII Acc65I AclI AflII Aor51HI AscI AseI AsnI Asp718I AvrII BciVI BfrI BlnI BsiEI BsiWI BspHI BspLU11I BssHII Bst1107I BstEII BstPI BstZ17I CpoI DraI EagI EclXI Eco47III Eco52I EcoO65I KpnI KspI MfeI MluI MunI NdeI NheI NotI NruI PacI PmeI PshBI Psp1406I PvuI RcaI RsrII SacII ScaI SexAI SfiI SnaBI SpeI SphI SwaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AflIII 5901 ACGTGT A^C[GA][CT]GT AgeI 1345 ACCGGT A^CCGGT Alw44I 5755 GTGCAC G^TGCAC ApaLI 5755 GTGCAC G^TGCAC BbrPI 5900 CACGTG CAC^GTG BcgI 192 CGATCCAGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 2397 TGATCA T^GATCA BsaAI 5900 CACGTG [CT]AC^GT[GA] Bsp1407I 1154 TGTACA T^GTACA BspDI 4567 ATCGAT AT^CGAT BsrDI 6497 CATTGC GCAATG(2/0) BsrGI 1154 TGTACA T^GTACA BstAPI 4278 GCAGACAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 5728 TTCGAA TT^CGAA ClaI 4567 ATCGAT AT^CGAT DraIII 4508 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 4281 GACAGTGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 2785 GAATTC G^AATTC EcoT22I 4063 ATGCAT ATGCA^T FbaI 2397 TGATCA T^GATCA FseI 5572 GGCCGGCC GGCCGG^CC HindIII 2464 AAGCTT A^AGCTT HpaI 3598 GTTAAC GTT^AAC NsiI 4063 ATGCAT ATGCA^T NspV 5728 TTCGAA TT^CGAA PaeR7I 3523 CTCGAG C^TCGAG PinAI 1345 ACCGGT A^CCGGT PmaCI 5900 CACGTG CAC^GTG PmlI 5900 CACGTG CAC^GTG SalI 3305 GTCGAC G^TCGAC SbfI 486 CCTGCAGG CCTGCA^GG SfuI 5728 TTCGAA TT^CGAA SgrAI 1344 CACCGGTG C[GA]^CCGG[CT]G SmlI 3523 CTCGAG C^T[CT][GA]AG Sse8387I 486 CCTGCAGG CCTGCA^GG SspBI 1154 TGTACA T^GTACA StuI 4737 AGGCCT AGG^CCT XbaI 306 TCTAGA T^CTAGA XhoI 3523 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccII 1 CGCG CG^CG 110 CGCG AccIII 1133 TCCGGA T^CCGGA 1181 TCCGGA AhdI 3308 GACGCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3565 GACTCCATGTC AocI 1104 CCTTAGG CC^T[ACGT]AGG 2223 CCTCAGG AspEI 3308 GACGCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3565 GACTCCATGTC AviII 3323 TGCGCA TGC^GCA 6521 TGCGCA BbeI 3470 GGCGCC GGCGC^C 6245 GGCGCC BseAI 1133 TCCGGA T^CCGGA 1181 TCCGGA BspEI 1133 TCCGGA T^CCGGA 1181 TCCGGA BsrBI 5763 CCGCTC CCGCTC(-3/-3) 6184 GAGCGG BstUI 1 CGCG CG^CG 110 CGCG Bsu36I 1104 CCTTAGG CC^T[ACGT]AGG 2223 CCTCAGG Eam1105I 3308 GACGCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3565 GACTCCATGTC Eco81I 1104 CCTTAGG CC^T[ACGT]AGG 2223 CCTCAGG EcoRV 1924 GATATC GAT^ATC 5518 GATATC FspI 3323 TGCGCA TGC^GCA 6521 TGCGCA HaeII 3470 GGCGCC [GA]GCGC^[CT] 6245 GGCGCC KasI 3470 GGCGCC G^GCGCC 6245 GGCGCC MroI 1133 TCCGGA T^CCGGA 1181 TCCGGA MvnI 1 CGCG CG^CG 110 CGCG NarI 3470 GGCGCC GG^CGCC 6245 GGCGCC PflMI 4507 CCACAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4890 CCATCAGCTGG PshAI 3016 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 5668 GACCTCAGTC SfoI 3470 GGCGCC GGC^GCC 6245 GGCGCC SmaI 3971 CCCGGG CCC^GGG 4543 CCCGGG SspI 937 AATATT AAT^ATT 1961 AATATT Van91I 4507 CCACAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4890 CCATCAGCTGG XmaCI 3971 CCCGGG C^CCGGG 4543 CCCGGG XmaI 3971 CCCGGG C^CCGGG 4543 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 137 enzymes) # ######################################### AccI AciI AcsI AcyI AfaI AluI AlwI AlwNI ApaI ApoI Asp700I AspHI AspI AvaI AvaII BalI BamHI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BssSI BstF5I BstNI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflFI PleI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XcmI XhoII XmnI