##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
AatII Acc65I AclI AflII Aor51HI
AscI AseI AsnI Asp718I AvrII
BciVI BfrI BlnI BsiEI BsiWI
BspHI BspLU11I BssHII Bst1107I BstEII
BstPI BstZ17I CpoI DraI EagI
EclXI Eco47III Eco52I EcoO65I KpnI
KspI MfeI MluI MunI NdeI
NheI NotI NruI PacI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SacII ScaI SexAI SfiI SnaBI
SpeI SphI SwaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AflIII 5901 ACGTGT A^C[GA][CT]GT
AgeI 1345 ACCGGT A^CCGGT
Alw44I 5755 GTGCAC G^TGCAC
ApaLI 5755 GTGCAC G^TGCAC
BbrPI 5900 CACGTG CAC^GTG
BcgI 192 CGATCCAGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2397 TGATCA T^GATCA
BsaAI 5900 CACGTG [CT]AC^GT[GA]
Bsp1407I 1154 TGTACA T^GTACA
BspDI 4567 ATCGAT AT^CGAT
BsrDI 6497 CATTGC GCAATG(2/0)
BsrGI 1154 TGTACA T^GTACA
BstAPI 4278 GCAGACAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 5728 TTCGAA TT^CGAA
ClaI 4567 ATCGAT AT^CGAT
DraIII 4508 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4281 GACAGTGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 2785 GAATTC G^AATTC
EcoT22I 4063 ATGCAT ATGCA^T
FbaI 2397 TGATCA T^GATCA
FseI 5572 GGCCGGCC GGCCGG^CC
HindIII 2464 AAGCTT A^AGCTT
HpaI 3598 GTTAAC GTT^AAC
NsiI 4063 ATGCAT ATGCA^T
NspV 5728 TTCGAA TT^CGAA
PaeR7I 3523 CTCGAG C^TCGAG
PinAI 1345 ACCGGT A^CCGGT
PmaCI 5900 CACGTG CAC^GTG
PmlI 5900 CACGTG CAC^GTG
SalI 3305 GTCGAC G^TCGAC
SbfI 486 CCTGCAGG CCTGCA^GG
SfuI 5728 TTCGAA TT^CGAA
SgrAI 1344 CACCGGTG C[GA]^CCGG[CT]G
SmlI 3523 CTCGAG C^T[CT][GA]AG
Sse8387I 486 CCTGCAGG CCTGCA^GG
SspBI 1154 TGTACA T^GTACA
StuI 4737 AGGCCT AGG^CCT
XbaI 306 TCTAGA T^CTAGA
XhoI 3523 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 1 CGCG CG^CG
110 CGCG
AccIII 1133 TCCGGA T^CCGGA
1181 TCCGGA
AhdI 3308 GACGCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3565 GACTCCATGTC
AocI 1104 CCTTAGG CC^T[ACGT]AGG
2223 CCTCAGG
AspEI 3308 GACGCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3565 GACTCCATGTC
AviII 3323 TGCGCA TGC^GCA
6521 TGCGCA
BbeI 3470 GGCGCC GGCGC^C
6245 GGCGCC
BseAI 1133 TCCGGA T^CCGGA
1181 TCCGGA
BspEI 1133 TCCGGA T^CCGGA
1181 TCCGGA
BsrBI 5763 CCGCTC CCGCTC(-3/-3)
6184 GAGCGG
BstUI 1 CGCG CG^CG
110 CGCG
Bsu36I 1104 CCTTAGG CC^T[ACGT]AGG
2223 CCTCAGG
Eam1105I 3308 GACGCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3565 GACTCCATGTC
Eco81I 1104 CCTTAGG CC^T[ACGT]AGG
2223 CCTCAGG
EcoRV 1924 GATATC GAT^ATC
5518 GATATC
FspI 3323 TGCGCA TGC^GCA
6521 TGCGCA
HaeII 3470 GGCGCC [GA]GCGC^[CT]
6245 GGCGCC
KasI 3470 GGCGCC G^GCGCC
6245 GGCGCC
MroI 1133 TCCGGA T^CCGGA
1181 TCCGGA
MvnI 1 CGCG CG^CG
110 CGCG
NarI 3470 GGCGCC GG^CGCC
6245 GGCGCC
PflMI 4507 CCACAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4890 CCATCAGCTGG
PshAI 3016 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5668 GACCTCAGTC
SfoI 3470 GGCGCC GGC^GCC
6245 GGCGCC
SmaI 3971 CCCGGG CCC^GGG
4543 CCCGGG
SspI 937 AATATT AAT^ATT
1961 AATATT
Van91I 4507 CCACAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4890 CCATCAGCTGG
XmaCI 3971 CCCGGG C^CCGGG
4543 CCCGGG
XmaI 3971 CCCGGG C^CCGGG
4543 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
Asp700I AspHI AspI AvaI AvaII
BalI BamHI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BglII BlpI BmyI BpmI Bpu1102I
BpuAI BsaBI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BssSI BstF5I BstNI BstXI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PflFI PleI PpuMI PstI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I XcmI
XhoII XmnI