##################################
# NO CUT ENZYMES (   65 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflIII      AgeI        Aor51HI     AscI        Asp718I     
AvrII       BbeI        BbrPI       BlnI        BsaHI       
BseAI       BsiWI       BspDI       BspEI       BspLU11I    
BssHII      Bst1107I    BstEII      BstPI       BstZ17I     
ClaI        CpoI        DraI        Eco47III    EcoO65I     
EcoRV       FseI        Hin1I       HindIII     HpaI        
KasI        KpnI        KspI        MfeI        MluI        
MroI        MunI        NarI        NdeI        NotI        
PacI        PaeR7I      PinAI       PmaCI       PmeI        
PmlI        PshAI       Psp1406I    PvuI        RsrII       
SacII       SalI        SfiI        SfoI        SgrAI       
SnaBI       SpeI        SwaI        XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AccI         4115  GTCTAC               GT^[AC][GT]AC
AflII        2592  CTTAAG               C^TTAAG
AhdI         6044  GACACCATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        638  GTGCAC               G^TGCAC
AocI          425  CCTGAGG              CC^T[ACGT]AGG
ApaI         4582  GGGCCC               GGGCC^C
ApaLI         638  GTGCAC               G^TGCAC
AseI         1272  ATTAAT               AT^TAAT
AsnI         1272  ATTAAT               AT^TAAT
AspEI        6044  GACACCATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII        2116  TGCGCA               TGC^GCA
BamHI        4053  GGATCC               G^GATCC
BcgI          853  GCATCAACTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI         2592  CTTAAG               C^TTAAG
BsaAI        1309  CACGTA               [CT]AC^GT[GA]
BsaBI        1583  GATGTTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI        1683  CGGCCG               CG[GA][CT]^CG
BsmBI        3669  GAGACG               CGTCTC(1/5)
BsrFI        3259  GCCGGC               [GA]^CCGG[CT]
Bsu36I        425  CCTGAGG              CC^T[ACGT]AGG
Cfr10I       3259  GCCGGC               [GA]^CCGG[CT]
DraIII       1260  CACCTCGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         6229  GACGTACAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         1683  CGGCCG               C^GGCCG
Eam1105I     6044  GACACCATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        1683  CGGCCG               C^GGCCG
Eco52I       1683  CGGCCG               C^GGCCG
Eco81I        425  CCTGAGG              CC^T[ACGT]AGG
EcoRI        6185  GAATTC               G^AATTC
EcoT22I      2185  ATGCAT               ATGCA^T
FspI         2116  TGCGCA               TGC^GCA
MamI         1583  GATGTTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI         3259  GCCGGC               GCC^GGC
NgoMIV       3259  GCCGGC               G^CCGGC
NruI         3452  TCGCGA               TCG^CGA
NsiI         2185  ATGCAT               ATGCA^T
PflMI        1259  CCACCTCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI        1272  ATTAAT               AT^TAAT
SbfI         4154  CCTGCAGG             CCTGCA^GG
ScaI          691  AGTACT               AGT^ACT
Sse8387I     4154  CCTGCAGG             CCTGCA^GG
SspI          891  AATATT               AAT^ATT
Van91I       1259  CCACCTCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

Asp700I      5075  GAAGGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             6027  GAATAAATTC          
BanI          885  GGTGCC               G^G[CT][GA]CC
             6297  GGTGCC              
BciVI        3581  GGATAC               GTATCC(6/5)
             4440  GTATCC              
BclI         2362  TGATCA               T^GATCA
             3086  TGATCA              
BglII          92  AGATCT               A^GATCT
             5122  AGATCT              
BsaWI          59  TCCGGT               [AT]^CCGG[AT]
             2845  TCCGGT              
Bsp1407I      535  TGTACA               T^GTACA
             2392  TGTACA              
BspHI         172  TCATGA               T^CATGA
             2359  TCATGA              
BsrGI         535  TGTACA               T^GTACA
             2392  TGTACA              
BssSI        4908  CACGAG               CACGAG(-5/-1)
             1263  CTCGTG              
BstAPI        554  GCATGAGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             3896  GCAGGATCTGC         
BstBI         246  TTCGAA               TT^CGAA
             5053  TTCGAA              
FbaI         2362  TGATCA               T^GATCA
             3086  TGATCA              
NcoI         5860  CCATGG               C^CATGG
             6514  CCATGG              
NheI         1142  GCTAGC               G^CTAGC
             5265  GCTAGC              
NspV          246  TTCGAA               TT^CGAA
             5053  TTCGAA              
RcaI          172  TCATGA               T^CATGA
             2359  TCATGA              
SexAI        4405  ACCTGGT              A^CC[AT]GGT
             4657  ACCTGGT             
SfuI          246  TTCGAA               TT^CGAA
             5053  TTCGAA              
SmaI         3934  CCCGGG               CCC^GGG
             4579  CCCGGG              
SphI          604  GCATGC               GCATG^C
             3994  GCATGC              
SspBI         535  TGTACA               T^GTACA
             2392  TGTACA              
TfiI         1418  GATTC                G^A[AT]TC
             3449  GATTC               
XmaCI        3934  CCCGGG               C^CCGGG
             4579  CCCGGG              
XmaI         3934  CCCGGG               C^CCGGG
             4579  CCCGGG              
XmnI         5075  GAAGGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             6027  GAATAAATTC          


#########################################
# 3 OR MORE CUT ENZYMES (  124 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApoI        AspHI       AspI        
AvaI        AvaII       BalI        BanII       BbsI        
BbvCI       BbvI        BcnI        BfaI        BglI        
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaI        BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsmI        
BsoBI       Bsp1286I    BspMI       BsrBI       BsrDI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
BstXI       BstYI       Cac8I       CelII       CfoI        
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        EcoNI       EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        HinP1I      
HincII      HindII      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NspI        PflFI       PleI        
PpuMI       PstI        PvuII       RsaI        SacI        
SapI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StuI        StyI        TaqI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
Tth111I     TthHB8I     XcmI        XhoII