################################## # NO CUT ENZYMES ( 65 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflIII AgeI Aor51HI AscI Asp718I AvrII BbeI BbrPI BlnI BsaHI BseAI BsiWI BspDI BspEI BspLU11I BssHII Bst1107I BstEII BstPI BstZ17I ClaI CpoI DraI Eco47III EcoO65I EcoRV FseI Hin1I HindIII HpaI KasI KpnI KspI MfeI MluI MroI MunI NarI NdeI NotI PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacII SalI SfiI SfoI SgrAI SnaBI SpeI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccI 4115 GTCTAC GT^[AC][GT]AC AflII 2592 CTTAAG C^TTAAG AhdI 6044 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 638 GTGCAC G^TGCAC AocI 425 CCTGAGG CC^T[ACGT]AGG ApaI 4582 GGGCCC GGGCC^C ApaLI 638 GTGCAC G^TGCAC AseI 1272 ATTAAT AT^TAAT AsnI 1272 ATTAAT AT^TAAT AspEI 6044 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 2116 TGCGCA TGC^GCA BamHI 4053 GGATCC G^GATCC BcgI 853 GCATCAACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 2592 CTTAAG C^TTAAG BsaAI 1309 CACGTA [CT]AC^GT[GA] BsaBI 1583 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 1683 CGGCCG CG[GA][CT]^CG BsmBI 3669 GAGACG CGTCTC(1/5) BsrFI 3259 GCCGGC [GA]^CCGG[CT] Bsu36I 425 CCTGAGG CC^T[ACGT]AGG Cfr10I 3259 GCCGGC [GA]^CCGG[CT] DraIII 1260 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 6229 GACGTACAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 1683 CGGCCG C^GGCCG Eam1105I 6044 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 1683 CGGCCG C^GGCCG Eco52I 1683 CGGCCG C^GGCCG Eco81I 425 CCTGAGG CC^T[ACGT]AGG EcoRI 6185 GAATTC G^AATTC EcoT22I 2185 ATGCAT ATGCA^T FspI 2116 TGCGCA TGC^GCA MamI 1583 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NaeI 3259 GCCGGC GCC^GGC NgoMIV 3259 GCCGGC G^CCGGC NruI 3452 TCGCGA TCG^CGA NsiI 2185 ATGCAT ATGCA^T PflMI 1259 CCACCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshBI 1272 ATTAAT AT^TAAT SbfI 4154 CCTGCAGG CCTGCA^GG ScaI 691 AGTACT AGT^ACT Sse8387I 4154 CCTGCAGG CCTGCA^GG SspI 891 AATATT AAT^ATT Van91I 1259 CCACCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### Asp700I 5075 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 6027 GAATAAATTC BanI 885 GGTGCC G^G[CT][GA]CC 6297 GGTGCC BciVI 3581 GGATAC GTATCC(6/5) 4440 GTATCC BclI 2362 TGATCA T^GATCA 3086 TGATCA BglII 92 AGATCT A^GATCT 5122 AGATCT BsaWI 59 TCCGGT [AT]^CCGG[AT] 2845 TCCGGT Bsp1407I 535 TGTACA T^GTACA 2392 TGTACA BspHI 172 TCATGA T^CATGA 2359 TCATGA BsrGI 535 TGTACA T^GTACA 2392 TGTACA BssSI 4908 CACGAG CACGAG(-5/-1) 1263 CTCGTG BstAPI 554 GCATGAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3896 GCAGGATCTGC BstBI 246 TTCGAA TT^CGAA 5053 TTCGAA FbaI 2362 TGATCA T^GATCA 3086 TGATCA NcoI 5860 CCATGG C^CATGG 6514 CCATGG NheI 1142 GCTAGC G^CTAGC 5265 GCTAGC NspV 246 TTCGAA TT^CGAA 5053 TTCGAA RcaI 172 TCATGA T^CATGA 2359 TCATGA SexAI 4405 ACCTGGT A^CC[AT]GGT 4657 ACCTGGT SfuI 246 TTCGAA TT^CGAA 5053 TTCGAA SmaI 3934 CCCGGG CCC^GGG 4579 CCCGGG SphI 604 GCATGC GCATG^C 3994 GCATGC SspBI 535 TGTACA T^GTACA 2392 TGTACA TfiI 1418 GATTC G^A[AT]TC 3449 GATTC XmaCI 3934 CCCGGG C^CCGGG 4579 CCCGGG XmaI 3934 CCCGGG C^CCGGG 4579 CCCGGG XmnI 5075 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 6027 GAATAAATTC ######################################### # 3 OR MORE CUT ENZYMES ( 124 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AspI AvaI AvaII BalI BanII BbsI BbvCI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflFI PleI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XcmI XhoII