##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflIII AgeI Aor51HI AscI Asp718I
AvrII BbeI BbrPI BlnI BsaHI
BseAI BsiWI BspDI BspEI BspLU11I
BssHII Bst1107I BstEII BstPI BstZ17I
ClaI CpoI DraI Eco47III EcoO65I
EcoRV FseI Hin1I HindIII HpaI
KasI KpnI KspI MfeI MluI
MroI MunI NarI NdeI NotI
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacII SalI SfiI SfoI SgrAI
SnaBI SpeI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 4115 GTCTAC GT^[AC][GT]AC
AflII 2592 CTTAAG C^TTAAG
AhdI 6044 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 638 GTGCAC G^TGCAC
AocI 425 CCTGAGG CC^T[ACGT]AGG
ApaI 4582 GGGCCC GGGCC^C
ApaLI 638 GTGCAC G^TGCAC
AseI 1272 ATTAAT AT^TAAT
AsnI 1272 ATTAAT AT^TAAT
AspEI 6044 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2116 TGCGCA TGC^GCA
BamHI 4053 GGATCC G^GATCC
BcgI 853 GCATCAACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2592 CTTAAG C^TTAAG
BsaAI 1309 CACGTA [CT]AC^GT[GA]
BsaBI 1583 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 1683 CGGCCG CG[GA][CT]^CG
BsmBI 3669 GAGACG CGTCTC(1/5)
BsrFI 3259 GCCGGC [GA]^CCGG[CT]
Bsu36I 425 CCTGAGG CC^T[ACGT]AGG
Cfr10I 3259 GCCGGC [GA]^CCGG[CT]
DraIII 1260 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 6229 GACGTACAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1683 CGGCCG C^GGCCG
Eam1105I 6044 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1683 CGGCCG C^GGCCG
Eco52I 1683 CGGCCG C^GGCCG
Eco81I 425 CCTGAGG CC^T[ACGT]AGG
EcoRI 6185 GAATTC G^AATTC
EcoT22I 2185 ATGCAT ATGCA^T
FspI 2116 TGCGCA TGC^GCA
MamI 1583 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 3259 GCCGGC GCC^GGC
NgoMIV 3259 GCCGGC G^CCGGC
NruI 3452 TCGCGA TCG^CGA
NsiI 2185 ATGCAT ATGCA^T
PflMI 1259 CCACCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 1272 ATTAAT AT^TAAT
SbfI 4154 CCTGCAGG CCTGCA^GG
ScaI 691 AGTACT AGT^ACT
Sse8387I 4154 CCTGCAGG CCTGCA^GG
SspI 891 AATATT AAT^ATT
Van91I 1259 CCACCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Asp700I 5075 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6027 GAATAAATTC
BanI 885 GGTGCC G^G[CT][GA]CC
6297 GGTGCC
BciVI 3581 GGATAC GTATCC(6/5)
4440 GTATCC
BclI 2362 TGATCA T^GATCA
3086 TGATCA
BglII 92 AGATCT A^GATCT
5122 AGATCT
BsaWI 59 TCCGGT [AT]^CCGG[AT]
2845 TCCGGT
Bsp1407I 535 TGTACA T^GTACA
2392 TGTACA
BspHI 172 TCATGA T^CATGA
2359 TCATGA
BsrGI 535 TGTACA T^GTACA
2392 TGTACA
BssSI 4908 CACGAG CACGAG(-5/-1)
1263 CTCGTG
BstAPI 554 GCATGAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3896 GCAGGATCTGC
BstBI 246 TTCGAA TT^CGAA
5053 TTCGAA
FbaI 2362 TGATCA T^GATCA
3086 TGATCA
NcoI 5860 CCATGG C^CATGG
6514 CCATGG
NheI 1142 GCTAGC G^CTAGC
5265 GCTAGC
NspV 246 TTCGAA TT^CGAA
5053 TTCGAA
RcaI 172 TCATGA T^CATGA
2359 TCATGA
SexAI 4405 ACCTGGT A^CC[AT]GGT
4657 ACCTGGT
SfuI 246 TTCGAA TT^CGAA
5053 TTCGAA
SmaI 3934 CCCGGG CCC^GGG
4579 CCCGGG
SphI 604 GCATGC GCATG^C
3994 GCATGC
SspBI 535 TGTACA T^GTACA
2392 TGTACA
TfiI 1418 GATTC G^A[AT]TC
3449 GATTC
XmaCI 3934 CCCGGG C^CCGGG
4579 CCCGGG
XmaI 3934 CCCGGG C^CCGGG
4579 CCCGGG
XmnI 5075 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6027 GAATAAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AspI
AvaI AvaII BalI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflFI PleI
PpuMI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I XcmI XhoII