##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII AccII AccIII AflIII AgeI
Aor51HI AscI AspI AviII BbeI
BbrPI BciVI BlpI Bpu1102I BsaAI
BsaWI BseAI BsiEI BsiWI BspDI
BspEI BspLU11I BssHII BstUI BstXI
CelII ClaI CpoI DsaI EagI
EclXI Eco47III Eco52I FspI HpaI
KasI KspI MluI MroI MvnI
NarI NcoI NdeI NotI NruI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PvuI PvuII RsrII
SacII SbfI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
StuI SwaI Tth111I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 2399 GGTACC G^GTACC
AclI 6029 AACGTT AA^CGTT
AcyI 6714 GGCGTC G[GA]^CG[CT]C
AhdI 1900 GACTGCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3102 GTGCAC G^TGCAC
AocI 5563 CCTTAGG CC^T[ACGT]AGG
ApaI 5974 GGGCCC GGGCC^C
ApaLI 3102 GTGCAC G^TGCAC
Asp718I 2399 GGTACC G^GTACC
AspEI 1900 GACTGCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 6191 CCTAGG C^CTAGG
BalI 135 TGGCCA TGG^CCA
BamHI 2311 GGATCC G^GATCC
BbvCI 3662 CCTCAGC CCTCAGC(-5/-2)
BclI 217 TGATCA T^GATCA
BlnI 6191 CCTAGG C^CTAGG
BsaHI 6714 GGCGTC G[GA]^CG[CT]C
BsaI 5607 GGTCTC GGTCTC(1/5)
BsmBI 6915 CGTCTC CGTCTC(1/5)
BsrBI 6710 GAGCGG CCGCTC(-3/-3)
BsrDI 4023 GCAATG GCAATG(2/0)
BssSI 4263 CTCGTG CACGAG(-5/-1)
BstBI 2694 TTCGAA TT^CGAA
Bsu36I 5563 CCTTAGG CC^T[ACGT]AGG
CfoI 3295 GCGC GCG^C
DrdI 5822 GACTTTCTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1900 GACTGCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 5563 CCTTAGG CC^T[ACGT]AGG
FauI 35 CCCGC CCCGC(4/6)
FbaI 217 TGATCA T^GATCA
FseI 23 GGCCGGCC GGCCGG^CC
HaeII 3294 AGCGCC [GA]GCGC^[CT]
HhaI 3295 GCGC GCG^C
Hin1I 6714 GGCGTC G[GA]^CG[CT]C
HinP1I 3295 GCGC G^CGC
HincII 3315 GTCGAC GT[CT]^[GA]AC
HindII 3315 GTCGAC GT[CT]^[GA]AC
KpnI 2399 GGTACC GGTAC^C
MluNI 135 TGGCCA TGG^CCA
MscI 135 TGGCCA TGG^CCA
NaeI 24 GCCGGC GCC^GGC
NgoMIV 24 GCCGGC G^CCGGC
NheI 5255 GCTAGC G^CTAGC
NspV 2694 TTCGAA TT^CGAA
PpuMI 3125 AGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 6029 AACGTT AA^CGTT
SalI 3315 GTCGAC G^TCGAC
ScaI 2586 AGTACT AGT^ACT
SexAI 4069 ACCTGGT A^CC[AT]GGT
SfuI 2694 TTCGAA TT^CGAA
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AlwNI 3540 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
5202 CAGGCTCTG
AseI 2227 ATTAAT AT^TAAT
6613 ATTAAT
AsnI 2227 ATTAAT AT^TAAT
6613 ATTAAT
AvaI 13 CTCGGG C^[CT]CG[GA]G
3807 CCCGAG
BcnI 770 CCCGG CC^[GC]GG
1759 CCCGG
BglI 3051 GCCCTCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4532 GCCAAGGTGGC
BsgI 4379 GTGCAG GTGCAG(16/14)
4754 GTGCAG
BsmI 1289 GAATGC GAATGC(1/-1)
6815 GCATTC
BsoBI 13 CTCGGG C^[CT]CG[GA]G
3807 CCCGAG
Bsp1407I 1717 TGTACA T^GTACA
4054 TGTACA
BspHI 1048 TCATGA T^CATGA
5043 TCATGA
BsrGI 1717 TGTACA T^GTACA
4054 TGTACA
Bst1107I 1343 GTATAC GTA^TAC
5748 GTATAC
BstAPI 691 GCACTCTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4523 GCATCCAATGC
BstEII 337 GGTTACC G^GT[ACGT]ACC
4065 GGTCACC
BstPI 337 GGTTACC G^GT[ACGT]ACC
4065 GGTCACC
BstZ17I 1343 GTATAC GTA^TAC
5748 GTATAC
DraI 463 TTTAAA TTT^AAA
1478 TTTAAA
EcoNI 596 CCTTCTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5833 CCTTTCTAAGG
EcoO65I 337 GGTTACC G^GT[ACGT]ACC
4065 GGTCACC
EcoRV 4367 GATATC GAT^ATC
6080 GATATC
HgaI 414 GACGC GACGC(5/10)
6715 GCGTC
MspA1I 4220 CAGCGG C[AC]G^C[GT]G
4808 CAGCGG
NciI 770 CCCGG CC^[GC]GG
1759 CCCGG
PflMI 200 CCAGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
559 CCATATTTTGG
PshAI 3248 GACTTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3846 GACAACTGTC
PshBI 2227 ATTAAT AT^TAAT
6613 ATTAAT
RcaI 1048 TCATGA T^CATGA
5043 TCATGA
SacI 3228 GAGCTC GAGCT^C
3515 GAGCTC
SspBI 1717 TGTACA T^GTACA
4054 TGTACA
SspI 1617 AATATT AAT^ATT
2021 AATATT
Van91I 200 CCAGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
559 CCATATTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AluI AlwI ApoI Asp700I AspHI
AvaII BanI BanII BbsI BbvI
BcgI BfaI BfrI BglII BmyI
BpmI BpuAI BsaBI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr10I
Cfr13I DdeI DpnII DraII DraIII
EaeI EarI EcoO109I EcoRI EcoRII
EcoT14I EcoT22I Fnu4HI FokI HaeIII
HapII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MfeI MflI
MnlI MseI MslI MspI MunI
MvaI MwoI NdeII NlaIII NlaIV
NsiI NspI PleI PstI RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XbaI XcmI XhoII XmnI