##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII AccIII AclI AflIII AgeI
AhdI AscI AseI AsnI AspEI
AspI AviII BbrPI BclI BsaBI
BseAI BsiWI Bsp1407I BspDI BspEI
BspLU11I BsrGI BssHII Bst1107I BstEII
BstPI BstZ17I ClaI EagI Eam1105I
EclXI Eco52I EcoO65I EcoRV EcoT22I
FbaI FspI HindIII HpaI MamI
MfeI MluI MroI MunI NdeI
NheI NotI NsiI PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI SalI ScaI
SfiI SgrAI SspBI SspI SwaI
Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 2467 GGTACC G^GTACC
AccI 5934 GTAGAC GT^[AC][GT]AC
Alw44I 5533 GTGCAC G^TGCAC
Aor51HI 4547 AGCGCT AGC^GCT
ApaLI 5533 GTGCAC G^TGCAC
Asp700I 1790 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2467 GGTACC G^GTACC
BamHI 6364 GGATCC G^GATCC
BbeI 4164 GGCGCC GGCGC^C
BcgI 3067 CGAGTATTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 748 GTATCC GTATCC(6/5)
BglII 1356 AGATCT A^GATCT
BsiEI 5246 CGACCG CG[GA][CT]^CG
BspHI 3316 TCATGA T^CATGA
BssSI 4080 CTCGTG CACGAG(-5/-1)
BstBI 1733 TTCGAA TT^CGAA
CpoI 4238 CGGACCG CG^G[AT]CCG
DraI 5317 TTTAAA TTT^AAA
DrdI 203 GACCCCAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4547 AGCGCT AGC^GCT
EcoRI 3137 GAATTC G^AATTC
HincII 983 GTTGAC GT[CT]^[GA]AC
HindII 983 GTTGAC GT[CT]^[GA]AC
KasI 4164 GGCGCC G^GCGCC
KpnI 2467 GGTACC GGTAC^C
KspI 724 CCGCGG CCGC^GG
NarI 4164 GGCGCC GG^CGCC
NruI 473 TCGCGA TCG^CGA
NspV 1733 TTCGAA TT^CGAA
RcaI 3316 TCATGA T^CATGA
RsrII 4238 CGGACCG CG^G[AT]CCG
SacII 724 CCGCGG CCGC^GG
SbfI 502 CCTGCAGG CCTGCA^GG
SexAI 2974 ACCAGGT A^CC[AT]GGT
SfoI 4164 GGCGCC GGC^GCC
SfuI 1733 TTCGAA TT^CGAA
SnaBI 58 TACGTA TAC^GTA
SpeI 217 ACTAGT A^CTAGT
Sse8387I 502 CCTGCAGG CCTGCA^GG
StuI 4466 AGGCCT AGG^CCT
XmnI 1790 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccII 474 CGCG CG^CG
725 CGCG
AflII 1448 CTTAAG C^TTAAG
5000 CTTAAG
AvrII 2999 CCTAGG C^CTAGG
5102 CCTAGG
BalI 667 TGGCCA TGG^CCA
5876 TGGCCA
BfrI 1448 CTTAAG C^TTAAG
5000 CTTAAG
BlnI 2999 CCTAGG C^CTAGG
5102 CCTAGG
BsaAI 58 TACGTA [CT]AC^GT[GA]
2171 TACGTG
BsaWI 2464 TCCGGT [AT]^CCGG[AT]
4675 ACCGGA
BsmI 1162 GAATGC GAATGC(1/-1)
5053 GAATGC
BsrBI 291 CCGCTC CCGCTC(-3/-3)
4658 GAGCGG
BstUI 474 CGCG CG^CG
725 CGCG
FseI 2111 GGCCGGCC GGCCGG^CC
2195 GGCCGGCC
MluNI 667 TGGCCA TGG^CCA
5876 TGGCCA
MscI 667 TGGCCA TGG^CCA
5876 TGGCCA
MvnI 474 CGCG CG^CG
725 CGCG
NcoI 430 CCATGG C^CATGG
439 CCATGG
NspI 150 GCATGC [GA]CATG^[CT]
1410 GCATGC
SphI 150 GCATGC GCATG^C
1410 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI AocI ApaI ApoI
AspHI AvaI AvaII BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrDI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV PaeR7I PflMI PleI PpuMI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoI XhoII
XmaCI XmaI