################################## # NO CUT ENZYMES ( 67 enzymes) # ################################## AatII AccIII AclI AflIII AgeI AhdI AscI AseI AsnI AspEI AspI AviII BbrPI BclI BsaBI BseAI BsiWI Bsp1407I BspDI BspEI BspLU11I BsrGI BssHII Bst1107I BstEII BstPI BstZ17I ClaI EagI Eam1105I EclXI Eco52I EcoO65I EcoRV EcoT22I FbaI FspI HindIII HpaI MamI MfeI MluI MroI MunI NdeI NheI NotI NsiI PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI SalI ScaI SfiI SgrAI SspBI SspI SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### Acc65I 2467 GGTACC G^GTACC AccI 5934 GTAGAC GT^[AC][GT]AC Alw44I 5533 GTGCAC G^TGCAC Aor51HI 4547 AGCGCT AGC^GCT ApaLI 5533 GTGCAC G^TGCAC Asp700I 1790 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 2467 GGTACC G^GTACC BamHI 6364 GGATCC G^GATCC BbeI 4164 GGCGCC GGCGC^C BcgI 3067 CGAGTATTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 748 GTATCC GTATCC(6/5) BglII 1356 AGATCT A^GATCT BsiEI 5246 CGACCG CG[GA][CT]^CG BspHI 3316 TCATGA T^CATGA BssSI 4080 CTCGTG CACGAG(-5/-1) BstBI 1733 TTCGAA TT^CGAA CpoI 4238 CGGACCG CG^G[AT]CCG DraI 5317 TTTAAA TTT^AAA DrdI 203 GACCCCAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 4547 AGCGCT AGC^GCT EcoRI 3137 GAATTC G^AATTC HincII 983 GTTGAC GT[CT]^[GA]AC HindII 983 GTTGAC GT[CT]^[GA]AC KasI 4164 GGCGCC G^GCGCC KpnI 2467 GGTACC GGTAC^C KspI 724 CCGCGG CCGC^GG NarI 4164 GGCGCC GG^CGCC NruI 473 TCGCGA TCG^CGA NspV 1733 TTCGAA TT^CGAA RcaI 3316 TCATGA T^CATGA RsrII 4238 CGGACCG CG^G[AT]CCG SacII 724 CCGCGG CCGC^GG SbfI 502 CCTGCAGG CCTGCA^GG SexAI 2974 ACCAGGT A^CC[AT]GGT SfoI 4164 GGCGCC GGC^GCC SfuI 1733 TTCGAA TT^CGAA SnaBI 58 TACGTA TAC^GTA SpeI 217 ACTAGT A^CTAGT Sse8387I 502 CCTGCAGG CCTGCA^GG StuI 4466 AGGCCT AGG^CCT XmnI 1790 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AccII 474 CGCG CG^CG 725 CGCG AflII 1448 CTTAAG C^TTAAG 5000 CTTAAG AvrII 2999 CCTAGG C^CTAGG 5102 CCTAGG BalI 667 TGGCCA TGG^CCA 5876 TGGCCA BfrI 1448 CTTAAG C^TTAAG 5000 CTTAAG BlnI 2999 CCTAGG C^CTAGG 5102 CCTAGG BsaAI 58 TACGTA [CT]AC^GT[GA] 2171 TACGTG BsaWI 2464 TCCGGT [AT]^CCGG[AT] 4675 ACCGGA BsmI 1162 GAATGC GAATGC(1/-1) 5053 GAATGC BsrBI 291 CCGCTC CCGCTC(-3/-3) 4658 GAGCGG BstUI 474 CGCG CG^CG 725 CGCG FseI 2111 GGCCGGCC GGCCGG^CC 2195 GGCCGGCC MluNI 667 TGGCCA TGG^CCA 5876 TGGCCA MscI 667 TGGCCA TGG^CCA 5876 TGGCCA MvnI 474 CGCG CG^CG 725 CGCG NcoI 430 CCATGG C^CATGG 439 CCATGG NspI 150 GCATGC [GA]CATG^[CT] 1410 GCATGC SphI 150 GCATGC GCATG^C 1410 GCATGC ######################################### # 3 OR MORE CUT ENZYMES ( 132 enzymes) # ######################################### AciI AcsI AcyI AfaI AluI AlwI AlwNI AocI ApaI ApoI AspHI AvaI AvaII BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV PaeR7I PflMI PleI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoI XhoII XmaCI XmaI