##################################
# NO CUT ENZYMES ( 35 enzymes) #
##################################
AclI AflII AgeI ApaI AscI
AseI AsnI BfrI BsiWI BssHII
BstBI EcoRV EcoT22I FseI HindIII
MfeI MunI NdeI NotI NruI
NsiI NspV PacI PinAI PmeI
PshBI Psp1406I PvuI SalI SfuI
SgrAI SnaBI SpeI SspI SwaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 6644 GGTACC G^GTACC
AccIII 4092 TCCGGA T^CCGGA
Asp718I 6644 GGTACC G^GTACC
AspI 1414 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 296 TGCGCA TGC^GCA
AvrII 3771 CCTAGG C^CTAGG
BbrPI 279 CACGTG CAC^GTG
BglII 4814 AGATCT A^GATCT
BlnI 3771 CCTAGG C^CTAGG
BseAI 4092 TCCGGA T^CCGGA
BspEI 4092 TCCGGA T^CCGGA
BspLU11I 4523 ACATGT A^CATGT
Bst1107I 6506 GTATAC GTA^TAC
BstZ17I 6506 GTATAC GTA^TAC
CpoI 4883 CGGACCG CG^G[AT]CCG
DraI 526 TTTAAA TTT^AAA
EagI 3086 CGGCCG C^GGCCG
EclXI 3086 CGGCCG C^GGCCG
Eco52I 3086 CGGCCG C^GGCCG
EcoRI 74 GAATTC G^AATTC
FspI 296 TGCGCA TGC^GCA
HpaI 1678 GTTAAC GTT^AAC
KpnI 6644 GGTACC GGTAC^C
KspI 3089 CCGCGG CCGC^GG
MluI 6515 ACGCGT A^CGCGT
MroI 4092 TCCGGA T^CCGGA
NcoI 6243 CCATGG C^CATGG
NheI 6193 GCTAGC G^CTAGC
PaeR7I 2105 CTCGAG C^TCGAG
PflFI 1414 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 279 CACGTG CAC^GTG
PmlI 279 CACGTG CAC^GTG
RsrII 4883 CGGACCG CG^G[AT]CCG
SacII 3089 CCGCGG CCGC^GG
SbfI 6607 CCTGCAGG CCTGCA^GG
SfiI 2568 GGCCAAGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 2113 GCATGC GCATG^C
Sse8387I 6607 CCTGCAGG CCTGCA^GG
Tth111I 1414 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2439 TCTAGA T^CTAGA
XhoI 2105 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AatII 1654 GACGTC GACGT^C
1840 GACGTC
AhdI 3643 GACCGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5016 GACACTGGGTC
Aor51HI 2772 AGCGCT AGC^GCT
3686 AGCGCT
AspEI 3643 GACCGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5016 GACACTGGGTC
BclI 251 TGATCA T^GATCA
1991 TGATCA
BsaBI 3555 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5941 GATGAACATC
BsiEI 3086 CGGCCG CG[GA][CT]^CG
3642 CGACCG
BsrDI 2676 CATTGC GCAATG(2/0)
6400 CATTGC
BssSI 2251 CACGAG CACGAG(-5/-1)
4319 CACGAG
BstAPI 816 GCAGGCCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
858 GCAGGGGGTGC
DrdI 40 GACAACAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2854 GACGCTGTGGTC
Eam1105I 3643 GACCGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5016 GACACTGGGTC
Eco47III 2772 AGCGCT AGC^GCT
3686 AGCGCT
FbaI 251 TGATCA T^GATCA
1991 TGATCA
MamI 3555 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5941 GATGAACATC
NaeI 154 GCCGGC GCC^GGC
5529 GCCGGC
NgoMIV 154 GCCGGC G^CCGGC
5529 GCCGGC
PshAI 6391 GACCGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
6427 GACCCCAGTC
SacI 421 GAGCTC GAGCT^C
6372 GAGCTC
ScaI 1607 AGTACT AGT^ACT
3974 AGTACT
SmaI 5600 CCCGGG CCC^GGG
6081 CCCGGG
XmaCI 5600 CCCGGG C^CCGGG
6081 CCCGGG
XmaI 5600 CCCGGG C^CCGGG
6081 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 159 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI Alw44I AlwI
AlwNI AocI ApaLI ApoI Asp700I
AspHI AvaI AvaII BalI BamHI
BanI BanII BbeI BbsI BbvCI
BbvI BcgI BciVI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspDI BspHI
BspMI BsrBI BsrFI BsrGI BsrI
BssKI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I ClaI
DdeI DpnII DraII DraIII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RcaI RsaI
SapI Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI SfoI SmlI SspBI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmnI