################################## # NO CUT ENZYMES ( 35 enzymes) # ################################## AclI AflII AgeI ApaI AscI AseI AsnI BfrI BsiWI BssHII BstBI EcoRV EcoT22I FseI HindIII MfeI MunI NdeI NotI NruI NsiI NspV PacI PinAI PmeI PshBI Psp1406I PvuI SalI SfuI SgrAI SnaBI SpeI SspI SwaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### Acc65I 6644 GGTACC G^GTACC AccIII 4092 TCCGGA T^CCGGA Asp718I 6644 GGTACC G^GTACC AspI 1414 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 296 TGCGCA TGC^GCA AvrII 3771 CCTAGG C^CTAGG BbrPI 279 CACGTG CAC^GTG BglII 4814 AGATCT A^GATCT BlnI 3771 CCTAGG C^CTAGG BseAI 4092 TCCGGA T^CCGGA BspEI 4092 TCCGGA T^CCGGA BspLU11I 4523 ACATGT A^CATGT Bst1107I 6506 GTATAC GTA^TAC BstZ17I 6506 GTATAC GTA^TAC CpoI 4883 CGGACCG CG^G[AT]CCG DraI 526 TTTAAA TTT^AAA EagI 3086 CGGCCG C^GGCCG EclXI 3086 CGGCCG C^GGCCG Eco52I 3086 CGGCCG C^GGCCG EcoRI 74 GAATTC G^AATTC FspI 296 TGCGCA TGC^GCA HpaI 1678 GTTAAC GTT^AAC KpnI 6644 GGTACC GGTAC^C KspI 3089 CCGCGG CCGC^GG MluI 6515 ACGCGT A^CGCGT MroI 4092 TCCGGA T^CCGGA NcoI 6243 CCATGG C^CATGG NheI 6193 GCTAGC G^CTAGC PaeR7I 2105 CTCGAG C^TCGAG PflFI 1414 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 279 CACGTG CAC^GTG PmlI 279 CACGTG CAC^GTG RsrII 4883 CGGACCG CG^G[AT]CCG SacII 3089 CCGCGG CCGC^GG SbfI 6607 CCTGCAGG CCTGCA^GG SfiI 2568 GGCCAAGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SphI 2113 GCATGC GCATG^C Sse8387I 6607 CCTGCAGG CCTGCA^GG Tth111I 1414 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 2439 TCTAGA T^CTAGA XhoI 2105 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AatII 1654 GACGTC GACGT^C 1840 GACGTC AhdI 3643 GACCGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5016 GACACTGGGTC Aor51HI 2772 AGCGCT AGC^GCT 3686 AGCGCT AspEI 3643 GACCGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5016 GACACTGGGTC BclI 251 TGATCA T^GATCA 1991 TGATCA BsaBI 3555 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 5941 GATGAACATC BsiEI 3086 CGGCCG CG[GA][CT]^CG 3642 CGACCG BsrDI 2676 CATTGC GCAATG(2/0) 6400 CATTGC BssSI 2251 CACGAG CACGAG(-5/-1) 4319 CACGAG BstAPI 816 GCAGGCCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 858 GCAGGGGGTGC DrdI 40 GACAACAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2854 GACGCTGTGGTC Eam1105I 3643 GACCGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5016 GACACTGGGTC Eco47III 2772 AGCGCT AGC^GCT 3686 AGCGCT FbaI 251 TGATCA T^GATCA 1991 TGATCA MamI 3555 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 5941 GATGAACATC NaeI 154 GCCGGC GCC^GGC 5529 GCCGGC NgoMIV 154 GCCGGC G^CCGGC 5529 GCCGGC PshAI 6391 GACCGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 6427 GACCCCAGTC SacI 421 GAGCTC GAGCT^C 6372 GAGCTC ScaI 1607 AGTACT AGT^ACT 3974 AGTACT SmaI 5600 CCCGGG CCC^GGG 6081 CCCGGG XmaCI 5600 CCCGGG C^CCGGG 6081 CCCGGG XmaI 5600 CCCGGG C^CCGGG 6081 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 159 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AflIII AluI Alw44I AlwI AlwNI AocI ApaLI ApoI Asp700I AspHI AvaI AvaII BalI BamHI BanI BanII BbeI BbsI BbvCI BbvI BcgI BciVI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspHI BspMI BsrBI BsrFI BsrGI BsrI BssKI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I ClaI DdeI DpnII DraII DraIII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RcaI RsaI SapI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SfoI SmlI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmnI