##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
Aor51HI AscI AseI AsnI AspI
BbrPI BfrI BglII BseAI BsiWI
BspDI BspEI BsrBI BssHII BstBI
ClaI CpoI DraIII Eco47III EcoRV
EcoT22I FseI HpaI MluI MroI
NdeI NruI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SalI
SapI SbfI SfiI SfuI SgrAI
SphI Sse8387I SspI SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 1313 GGTACC G^GTACC
AhdI 6545 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 1313 GGTACC G^GTACC
AspEI 6545 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 4936 TGCGCA TGC^GCA
BbeI 1404 GGCGCC GGCGC^C
BlpI 6856 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 6856 GCTGAGC GC^T[ACGT]AGC
BsaBI 3281 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 3564 ACCGGA [AT]^CCGG[AT]
BsiEI 67 CGGCCG CG[GA][CT]^CG
Bsp1407I 1880 TGTACA T^GTACA
BspLU11I 6118 ACATGT A^CATGT
BsrGI 1880 TGTACA T^GTACA
Bst1107I 1415 GTATAC GTA^TAC
BstZ17I 1415 GTATAC GTA^TAC
CelII 6856 GCTGAGC GC^T[ACGT]AGC
DraI 5831 TTTAAA TTT^AAA
EagI 67 CGGCCG C^GGCCG
Eam1105I 6545 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 67 CGGCCG C^GGCCG
Eco52I 67 CGGCCG C^GGCCG
EcoRI 658 GAATTC G^AATTC
FspI 4936 TGCGCA TGC^GCA
HincII 4548 GTTGAC GT[CT]^[GA]AC
HindII 4548 GTTGAC GT[CT]^[GA]AC
KasI 1404 GGCGCC G^GCGCC
KpnI 1313 GGTACC GGTAC^C
KspI 64 CCGCGG CCGC^GG
MamI 3281 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 1404 GGCGCC GG^CGCC
NotI 66 GCGGCCGC GC^GGCCGC
NspI 6118 ACATGT [GA]CATG^[CT]
SacII 64 CCGCGG CCGC^GG
ScaI 3031 AGTACT AGT^ACT
SfoI 1404 GGCGCC GGC^GCC
SnaBI 551 TACGTA TAC^GTA
SpeI 11 ACTAGT A^CTAGT
SspBI 1880 TGTACA T^GTACA
XbaI 3104 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 324 GACGCC G[GA]^CG[CT]C
1404 GGCGCC
AflIII 6118 ACATGT A^C[GA][CT]GT
6792 ACGTGT
BclI 830 TGATCA T^GATCA
3265 TGATCA
BglI 1778 GCCAGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5073 GCCAACCAGGC
BsaAI 551 TACGTA [CT]AC^GT[GA]
5231 CACGTA
BsaHI 324 GACGCC G[GA]^CG[CT]C
1404 GGCGCC
BsmBI 2918 CGTCTC CGTCTC(1/5)
3458 CGTCTC
BsmI 6583 GAATGC GAATGC(1/-1)
349 GCATTC
BstEII 1706 GGTCACC G^GT[ACGT]ACC
1955 GGTGACC
BstPI 1706 GGTCACC G^GT[ACGT]ACC
1955 GGTGACC
EcoNI 902 CCTGACACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4554 CCTAAGACAGG
EcoO65I 1706 GGTCACC G^GT[ACGT]ACC
1955 GGTGACC
FbaI 830 TGATCA T^GATCA
3265 TGATCA
Hin1I 324 GACGCC G[GA]^CG[CT]C
1404 GGCGCC
HindIII 75 AAGCTT A^AGCTT
139 AAGCTT
MfeI 475 CAATTG C^AATTG
6099 CAATTG
MunI 475 CAATTG C^AATTG
6099 CAATTG
NaeI 20 GCCGGC GCC^GGC
2383 GCCGGC
NgoMIV 20 GCCGGC G^CCGGC
2383 GCCGGC
NheI 5186 GCTAGC G^CTAGC
6659 GCTAGC
PaeR7I 1636 CTCGAG C^TCGAG
5934 CTCGAG
PshAI 5456 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
6367 GACTTCGGTC
SexAI 952 ACCTGGT A^CC[AT]GGT
1735 ACCTGGT
StuI 2134 AGGCCT AGG^CCT
3614 AGGCCT
XcmI 1430 CCATGGGGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4220 CCACGACAGCTCTGG
XhoI 1636 CTCGAG C^TCGAG
5934 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI Alw44I AlwI AlwNI AocI
ApaI ApaLI ApoI Asp700I AspHI
AvaI AvaII AvrII BalI BamHI
BanI BanII BbsI BbvCI BbvI
BcgI BciVI BcnI BfaI BlnI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspHI
BspMI BsrDI BsrFI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DrdI DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RcaI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII XmaCI
XmaI XmnI