################################## # NO CUT ENZYMES ( 55 enzymes) # ################################## AatII AccIII AclI AflII AgeI Aor51HI AscI AseI AsnI AspI BbrPI BfrI BglII BseAI BsiWI BspDI BspEI BsrBI BssHII BstBI ClaI CpoI DraIII Eco47III EcoRV EcoT22I FseI HpaI MluI MroI NdeI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SalI SapI SbfI SfiI SfuI SgrAI SphI Sse8387I SspI SwaI Tth111I ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### Acc65I 1313 GGTACC G^GTACC AhdI 6545 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp718I 1313 GGTACC G^GTACC AspEI 6545 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 4936 TGCGCA TGC^GCA BbeI 1404 GGCGCC GGCGC^C BlpI 6856 GCTGAGC GC^T[ACGT]AGC Bpu1102I 6856 GCTGAGC GC^T[ACGT]AGC BsaBI 3281 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 3564 ACCGGA [AT]^CCGG[AT] BsiEI 67 CGGCCG CG[GA][CT]^CG Bsp1407I 1880 TGTACA T^GTACA BspLU11I 6118 ACATGT A^CATGT BsrGI 1880 TGTACA T^GTACA Bst1107I 1415 GTATAC GTA^TAC BstZ17I 1415 GTATAC GTA^TAC CelII 6856 GCTGAGC GC^T[ACGT]AGC DraI 5831 TTTAAA TTT^AAA EagI 67 CGGCCG C^GGCCG Eam1105I 6545 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 67 CGGCCG C^GGCCG Eco52I 67 CGGCCG C^GGCCG EcoRI 658 GAATTC G^AATTC FspI 4936 TGCGCA TGC^GCA HincII 4548 GTTGAC GT[CT]^[GA]AC HindII 4548 GTTGAC GT[CT]^[GA]AC KasI 1404 GGCGCC G^GCGCC KpnI 1313 GGTACC GGTAC^C KspI 64 CCGCGG CCGC^GG MamI 3281 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NarI 1404 GGCGCC GG^CGCC NotI 66 GCGGCCGC GC^GGCCGC NspI 6118 ACATGT [GA]CATG^[CT] SacII 64 CCGCGG CCGC^GG ScaI 3031 AGTACT AGT^ACT SfoI 1404 GGCGCC GGC^GCC SnaBI 551 TACGTA TAC^GTA SpeI 11 ACTAGT A^CTAGT SspBI 1880 TGTACA T^GTACA XbaI 3104 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcyI 324 GACGCC G[GA]^CG[CT]C 1404 GGCGCC AflIII 6118 ACATGT A^C[GA][CT]GT 6792 ACGTGT BclI 830 TGATCA T^GATCA 3265 TGATCA BglI 1778 GCCAGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 5073 GCCAACCAGGC BsaAI 551 TACGTA [CT]AC^GT[GA] 5231 CACGTA BsaHI 324 GACGCC G[GA]^CG[CT]C 1404 GGCGCC BsmBI 2918 CGTCTC CGTCTC(1/5) 3458 CGTCTC BsmI 6583 GAATGC GAATGC(1/-1) 349 GCATTC BstEII 1706 GGTCACC G^GT[ACGT]ACC 1955 GGTGACC BstPI 1706 GGTCACC G^GT[ACGT]ACC 1955 GGTGACC EcoNI 902 CCTGACACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4554 CCTAAGACAGG EcoO65I 1706 GGTCACC G^GT[ACGT]ACC 1955 GGTGACC FbaI 830 TGATCA T^GATCA 3265 TGATCA Hin1I 324 GACGCC G[GA]^CG[CT]C 1404 GGCGCC HindIII 75 AAGCTT A^AGCTT 139 AAGCTT MfeI 475 CAATTG C^AATTG 6099 CAATTG MunI 475 CAATTG C^AATTG 6099 CAATTG NaeI 20 GCCGGC GCC^GGC 2383 GCCGGC NgoMIV 20 GCCGGC G^CCGGC 2383 GCCGGC NheI 5186 GCTAGC G^CTAGC 6659 GCTAGC PaeR7I 1636 CTCGAG C^TCGAG 5934 CTCGAG PshAI 5456 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 6367 GACTTCGGTC SexAI 952 ACCTGGT A^CC[AT]GGT 1735 ACCTGGT StuI 2134 AGGCCT AGG^CCT 3614 AGGCCT XcmI 1430 CCATGGGGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4220 CCACGACAGCTCTGG XhoI 1636 CTCGAG C^TCGAG 5934 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 137 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AluI Alw44I AlwI AlwNI AocI ApaI ApaLI ApoI Asp700I AspHI AvaI AvaII AvrII BalI BamHI BanI BanII BbsI BbvCI BbvI BcgI BciVI BcnI BfaI BlnI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspHI BspMI BsrDI BsrFI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DrdI DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV PflMI PleI PpuMI PstI PvuII RcaI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmaCI XmaI XmnI