##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AatII AclI AflII AscI AviII
BamHI BbeI BbrPI BfrI BglII
BsaAI BsiWI Bsp1407I BspDI BsrDI
BsrGI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI EcoRI FseI FspI
KasI KspI MfeI MluI MunI
NarI NotI NruI NspV PacI
PaeR7I PmaCI PmeI PmlI Psp1406I
PvuI RsrII SacII SalI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SspBI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccIII 717 TCCGGA T^CCGGA
AcyI 3042 GGCGTC G[GA]^CG[CT]C
AgeI 6498 ACCGGT A^CCGGT
ApaI 4232 GGGCCC GGGCC^C
AseI 196 ATTAAT AT^TAAT
AsnI 196 ATTAAT AT^TAAT
Asp700I 4429 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BcgI 739 GCAGTATCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaBI 4657 GATATGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 3042 GGCGTC G[GA]^CG[CT]C
BseAI 717 TCCGGA T^CCGGA
BsmBI 1986 CGTCTC CGTCTC(1/5)
BspEI 717 TCCGGA T^CCGGA
BspLU11I 2977 ACATGT A^CATGT
BsrBI 5167 GAGCGG CCGCTC(-3/-3)
DrdI 1301 GACACTGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 5517 CGGCCG C^GGCCG
EclXI 5517 CGGCCG C^GGCCG
Eco52I 5517 CGGCCG C^GGCCG
EcoRV 2439 GATATC GAT^ATC
Hin1I 3042 GGCGTC G[GA]^CG[CT]C
HpaI 3961 GTTAAC GTT^AAC
MamI 4657 GATATGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 717 TCCGGA T^CCGGA
NaeI 5515 GCCGGC GCC^GGC
NdeI 4324 CATATG CA^TATG
NgoMIV 5515 GCCGGC G^CCGGC
PinAI 6498 ACCGGT A^CCGGT
PshBI 196 ATTAAT AT^TAAT
SbfI 1788 CCTGCAGG CCTGCA^GG
SmaI 2879 CCCGGG CCC^GGG
Sse8387I 1788 CCTGCAGG CCTGCA^GG
SspI 6355 AATATT AAT^ATT
XbaI 2435 TCTAGA T^CTAGA
XmaCI 2879 CCCGGG C^CCGGG
XmaI 2879 CCCGGG C^CCGGG
XmnI 4429 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Acc65I 5639 GGTACC G^GTACC
6289 GGTACC
AhdI 286 GACTGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
626 GACCTGGAGTC
Alw44I 798 GTGCAC G^TGCAC
916 GTGCAC
ApaLI 798 GTGCAC G^TGCAC
916 GTGCAC
Asp718I 5639 GGTACC G^GTACC
6289 GGTACC
AspEI 286 GACTGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
626 GACCTGGAGTC
AspI 209 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4283 GACTCAGTC
BbvCI 5188 GCTGAGG CCTCAGC(-5/-2)
6135 GCTGAGG
BciVI 3631 GTATCC GTATCC(6/5)
4975 GTATCC
BclI 682 TGATCA T^GATCA
4129 TGATCA
BsiEI 5517 CGGCCG CG[GA][CT]^CG
6711 CGACCG
BsmI 4963 GAATGC GAATGC(1/-1)
4433 GCATTC
BsrFI 5515 GCCGGC [GA]^CCGG[CT]
6498 ACCGGT
BstYI 4447 AGATCC [GA]^GATC[CT]
5784 AGATCC
Cfr10I 5515 GCCGGC [GA]^CCGG[CT]
6498 ACCGGT
Eam1105I 286 GACTGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
626 GACCTGGAGTC
EcoNI 6070 CCTCCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
6131 CCTTGCTGAGG
FbaI 682 TGATCA T^GATCA
4129 TGATCA
HgaI 1008 GACGC GACGC(5/10)
3043 GCGTC
KpnI 5639 GGTACC GGTAC^C
6289 GGTACC
MflI 4447 AGATCC [GA]^GATC[CT]
5784 AGATCC
PflFI 209 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4283 GACTCAGTC
SapI 519 GAAGAGC GCTCTTC(1/4)
763 GAAGAGC
SphI 5362 GCATGC GCATG^C
5740 GCATGC
Tth111I 209 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4283 GACTCAGTC
XhoII 4447 AGATCC [GA]^GATC[CT]
5784 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 140 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI AocI
Aor51HI ApoI AspHI AvaI AvaII
AvrII BalI BanI BanII BbsI
BbvI BcnI BfaI BglI BlnI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspHI BspMI BsrI
BssKI BssSI BstAPI BstEII BstF5I
BstNI BstPI BstUI BstXI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraI DraII DraIII DsaI
EaeI EarI Eco47III Eco81I EcoO109I
EcoO65I EcoRII EcoT14I EcoT22I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NheI NlaIII
NlaIV NsiI NspI PflMI PleI
PpuMI PshAI PstI PvuII RcaI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI