##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AatII AccIII AflII AflIII AgeI
AhdI AscI AseI AsnI AspEI
AviII BfrI BseAI BsiWI BspDI
BspEI BspLU11I BsrFI BssHII Bst1107I
BstBI BstZ17I Cfr10I ClaI CpoI
DraI Eam1105I EcoRV FseI FspI
HpaI MfeI MluI MroI MunI
NaeI NgoMIV NheI NotI NruI
NspV PacI PinAI PmeI PshBI
PvuI RsrII SalI SapI SfiI
SfuI SgrAI SnaBI SpeI SphI
SwaI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AclI 1575 AACGTT AA^CGTT
AspI 5874 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 5396 CACGTG CAC^GTG
BcgI 2989 CGAGATGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2145 TGATCA T^GATCA
BsaAI 5396 CACGTG [CT]AC^GT[GA]
BsiEI 1740 CGGCCG CG[GA][CT]^CG
BsmBI 6578 CGTCTC CGTCTC(1/5)
Bsp1407I 307 TGTACA T^GTACA
BsrDI 5996 GCAATG GCAATG(2/0)
BsrGI 307 TGTACA T^GTACA
BstEII 1748 GGTCACC G^GT[ACGT]ACC
BstPI 1748 GGTCACC G^GT[ACGT]ACC
EagI 1740 CGGCCG C^GGCCG
EclXI 1740 CGGCCG C^GGCCG
Eco52I 1740 CGGCCG C^GGCCG
EcoO65I 1748 GGTCACC G^GT[ACGT]ACC
FbaI 2145 TGATCA T^GATCA
KspI 4487 CCGCGG CCGC^GG
PaeR7I 477 CTCGAG C^TCGAG
PflFI 5874 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 5396 CACGTG CAC^GTG
PmlI 5396 CACGTG CAC^GTG
Psp1406I 1575 AACGTT AA^CGTT
SacII 4487 CCGCGG CCGC^GG
SspBI 307 TGTACA T^GTACA
SspI 5235 AATATT AAT^ATT
Tth111I 5874 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 477 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 5777 GGTACC G^GTACC
7386 GGTACC
AccI 3368 GTCTAC GT^[AC][GT]AC
4787 GTAGAC
AccII 590 CGCG CG^CG
4488 CGCG
Alw44I 3527 GTGCAC G^TGCAC
5393 GTGCAC
ApaLI 3527 GTGCAC G^TGCAC
5393 GTGCAC
Asp700I 4716 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5232 GAAAATATTC
Asp718I 5777 GGTACC G^GTACC
7386 GGTACC
AvrII 1837 CCTAGG C^CTAGG
4173 CCTAGG
BamHI 5165 GGATCC G^GATCC
6253 GGATCC
BbeI 3135 GGCGCC GGCGC^C
3949 GGCGCC
BbvCI 4536 CCTCAGC CCTCAGC(-5/-2)
4112 GCTGAGG
BciVI 5903 GTATCC GTATCC(6/5)
7258 GTATCC
BglII 7104 AGATCT A^GATCT
7175 AGATCT
BlnI 1837 CCTAGG C^CTAGG
4173 CCTAGG
BsaBI 1402 GATCCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5447 GATCCTTATC
BsaWI 5162 ACCGGA [AT]^CCGG[AT]
6335 ACCGGA
BspHI 495 TCATGA T^CATGA
1398 TCATGA
BssSI 3187 CACGAG CACGAG(-5/-1)
501 CTCGTG
BstUI 590 CGCG CG^CG
4488 CGCG
DrdI 1292 GACAGGTGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4690 GACAGGGCTGTC
EcoRI 2366 GAATTC G^AATTC
5065 GAATTC
EcoT22I 3730 ATGCAT ATGCA^T
5818 ATGCAT
HindIII 580 AAGCTT A^AGCTT
6115 AAGCTT
KasI 3135 GGCGCC G^GCGCC
3949 GGCGCC
KpnI 5777 GGTACC GGTAC^C
7386 GGTACC
MamI 1402 GATCCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5447 GATCCTTATC
MvnI 590 CGCG CG^CG
4488 CGCG
NarI 3135 GGCGCC GG^CGCC
3949 GGCGCC
NdeI 73 CATATG CA^TATG
5186 CATATG
NsiI 3730 ATGCAT ATGCA^T
5818 ATGCAT
RcaI 495 TCATGA T^CATGA
1398 TCATGA
SbfI 1312 CCTGCAGG CCTGCA^GG
3394 CCTGCAGG
SexAI 4743 ACCAGGT A^CC[AT]GGT
5875 ACCTGGT
SfoI 3135 GGCGCC GGC^GCC
3949 GGCGCC
SmaI 4392 CCCGGG CCC^GGG
6510 CCCGGG
Sse8387I 1312 CCTGCAGG CCTGCA^GG
3394 CCTGCAGG
TfiI 914 GATTC G^A[AT]TC
5562 GATTC
XmaCI 4392 CCCGGG C^CCGGG
6510 CCCGGG
XmaI 4392 CCCGGG C^CCGGG
6510 CCCGGG
XmnI 4716 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5232 GAAAATATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI AocI Aor51HI ApaI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrBI BsrI BssKI BstAPI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI Eco47III Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PshAI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
StuI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XbaI XcmI XhoII