##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AflII AgeI Aor51HI ApaI AscI
AseI AsnI Asp700I AviII AvrII
BbeI BbrPI BcgI BclI BfrI
BlnI BsaAI BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI Bsp1407I BspDI
BspEI BspHI BsrBI BsrFI BsrGI
BssHII Bst1107I BstBI BstUI BstZ17I
CfoI Cfr10I ClaI CpoI EagI
EclXI Eco47III Eco52I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
HhaI Hin1I HinP1I HindIII KasI
KspI MamI MluI MroI MvnI
NaeI NarI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SacII ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI SspBI SspI SwaI
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
Acc65I 3296 GGTACC G^GTACC
AcsI 2422 AAATTC [GA]^AATT[CT]
ApoI 2422 AAATTC [GA]^AATT[CT]
Asp718I 3296 GGTACC G^GTACC
AvaI 2808 CCCGGG C^[CT]CG[GA]G
BglI 844 GCCTTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsoBI 2808 CCCGGG C^[CT]CG[GA]G
BssSI 2508 CACGAG CACGAG(-5/-1)
BstEII 1896 GGTGACC G^GT[ACGT]ACC
BstPI 1896 GGTGACC G^GT[ACGT]ACC
DraI 1234 TTTAAA TTT^AAA
DrdI 760 GACACACTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 2541 GAAGAG CTCTTC(1/4)
EcoO65I 1896 GGTGACC G^GT[ACGT]ACC
FauI 659 CCCGC CCCGC(4/6)
HpaI 1317 GTTAAC GTT^AAC
KpnI 3296 GGTACC GGTAC^C
Ksp632I 2541 GAAGAG CTCTTC(1/4)
MfeI 2566 CAATTG C^AATTG
MunI 2566 CAATTG C^AATTG
NdeI 2833 CATATG CA^TATG
NheI 964 GCTAGC G^CTAGC
SalI 1814 GTCGAC G^TCGAC
SapI 2541 GAAGAGC GCTCTTC(1/4)
SbfI 705 CCTGCAGG CCTGCA^GG
SexAI 3260 ACCTGGT A^CC[AT]GGT
SmaI 2808 CCCGGG CCC^GGG
Sse8387I 705 CCTGCAGG CCTGCA^GG
StuI 855 AGGCCT AGG^CCT
XmaCI 2808 CCCGGG C^CCGGG
XmaI 2808 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 815 GTAGAC GT^[AC][GT]AC
1814 GTCGAC
AciI 660 CCGC CCGC(-3/-1)
935 CCGC
AhdI 2657 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3216 GACCAAGTGTC
Alw44I 381 GTGCAC G^TGCAC
2852 GTGCAC
AocI 549 CCTTAGG CC^T[ACGT]AGG
3291 CCTCAGG
ApaLI 381 GTGCAC G^TGCAC
2852 GTGCAC
AspEI 2657 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3216 GACCAAGTGTC
AspI 1673 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2246 GACTGGGTC
BamHI 1718 GGATCC G^GATCC
3002 GGATCC
BciVI 650 GTATCC GTATCC(6/5)
3160 GTATCC
BglII 2077 AGATCT A^GATCT
3077 AGATCT
BsaI 1018 GAGACC GGTCTC(1/5)
3051 GGTCTC
BsmBI 349 CGTCTC CGTCTC(1/5)
2128 GAGACG
BsmI 42 GAATGC GAATGC(1/-1)
3136 GAATGC
BstAPI 367 GCAATACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2613 GCAGTGCCTGC
BstXI 169 CCAGGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1148 CCATTCACCTGG
Bsu36I 549 CCTTAGG CC^T[ACGT]AGG
3291 CCTCAGG
Eam1105I 2657 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3216 GACCAAGTGTC
Eco81I 549 CCTTAGG CC^T[ACGT]AGG
3291 CCTCAGG
EcoNI 2820 CCTCAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3180 CCTGAGCCAGG
HincII 1317 GTTAAC GT[CT]^[GA]AC
1814 GTCGAC
HindII 1317 GTTAAC GT[CT]^[GA]AC
1814 GTCGAC
MaeII 348 ACGT A^CGT
880 ACGT
MseI 1235 TTAA T^TAA
1318 TTAA
MslI 1135 CACAGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2347 CACTTAGATG
NcoI 153 CCATGG C^CATGG
2291 CCATGG
PflFI 1673 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2246 GACTGGGTC
PshAI 2430 GACTGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2971 GACCTCAGTC
SacI 3066 GAGCTC GAGCT^C
3205 GAGCTC
SmlI 1417 CTCAAG C^T[CT][GA]AG
2362 CTTGAG
Tru9I 1235 TTAA T^TAA
1318 TTAA
Tth111I 1673 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2246 GACTGGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AfaI AflIII AluI AlwI AlwNI
AspHI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspLU11I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I CelII Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HgaI HinfI
HpaII HphI ItaI MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII