################################## # NO CUT ENZYMES ( 98 enzymes) # ################################## AatII AccII AccIII AclI AcyI AflII AgeI Aor51HI ApaI AscI AseI AsnI Asp700I AviII AvrII BbeI BbrPI BcgI BclI BfrI BlnI BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI Bsp1407I BspDI BspEI BspHI BsrBI BsrFI BsrGI BssHII Bst1107I BstBI BstUI BstZ17I CfoI Cfr10I ClaI CpoI EagI EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HhaI Hin1I HinP1I HindIII KasI KspI MamI MluI MroI MvnI NaeI NarI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacII ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI SspBI SspI SwaI XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### Acc65I 3296 GGTACC G^GTACC AcsI 2422 AAATTC [GA]^AATT[CT] ApoI 2422 AAATTC [GA]^AATT[CT] Asp718I 3296 GGTACC G^GTACC AvaI 2808 CCCGGG C^[CT]CG[GA]G BglI 844 GCCTTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsoBI 2808 CCCGGG C^[CT]CG[GA]G BssSI 2508 CACGAG CACGAG(-5/-1) BstEII 1896 GGTGACC G^GT[ACGT]ACC BstPI 1896 GGTGACC G^GT[ACGT]ACC DraI 1234 TTTAAA TTT^AAA DrdI 760 GACACACTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 2541 GAAGAG CTCTTC(1/4) EcoO65I 1896 GGTGACC G^GT[ACGT]ACC FauI 659 CCCGC CCCGC(4/6) HpaI 1317 GTTAAC GTT^AAC KpnI 3296 GGTACC GGTAC^C Ksp632I 2541 GAAGAG CTCTTC(1/4) MfeI 2566 CAATTG C^AATTG MunI 2566 CAATTG C^AATTG NdeI 2833 CATATG CA^TATG NheI 964 GCTAGC G^CTAGC SalI 1814 GTCGAC G^TCGAC SapI 2541 GAAGAGC GCTCTTC(1/4) SbfI 705 CCTGCAGG CCTGCA^GG SexAI 3260 ACCTGGT A^CC[AT]GGT SmaI 2808 CCCGGG CCC^GGG Sse8387I 705 CCTGCAGG CCTGCA^GG StuI 855 AGGCCT AGG^CCT XmaCI 2808 CCCGGG C^CCGGG XmaI 2808 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccI 815 GTAGAC GT^[AC][GT]AC 1814 GTCGAC AciI 660 CCGC CCGC(-3/-1) 935 CCGC AhdI 2657 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3216 GACCAAGTGTC Alw44I 381 GTGCAC G^TGCAC 2852 GTGCAC AocI 549 CCTTAGG CC^T[ACGT]AGG 3291 CCTCAGG ApaLI 381 GTGCAC G^TGCAC 2852 GTGCAC AspEI 2657 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3216 GACCAAGTGTC AspI 1673 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2246 GACTGGGTC BamHI 1718 GGATCC G^GATCC 3002 GGATCC BciVI 650 GTATCC GTATCC(6/5) 3160 GTATCC BglII 2077 AGATCT A^GATCT 3077 AGATCT BsaI 1018 GAGACC GGTCTC(1/5) 3051 GGTCTC BsmBI 349 CGTCTC CGTCTC(1/5) 2128 GAGACG BsmI 42 GAATGC GAATGC(1/-1) 3136 GAATGC BstAPI 367 GCAATACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2613 GCAGTGCCTGC BstXI 169 CCAGGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1148 CCATTCACCTGG Bsu36I 549 CCTTAGG CC^T[ACGT]AGG 3291 CCTCAGG Eam1105I 2657 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3216 GACCAAGTGTC Eco81I 549 CCTTAGG CC^T[ACGT]AGG 3291 CCTCAGG EcoNI 2820 CCTCAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3180 CCTGAGCCAGG HincII 1317 GTTAAC GT[CT]^[GA]AC 1814 GTCGAC HindII 1317 GTTAAC GT[CT]^[GA]AC 1814 GTCGAC MaeII 348 ACGT A^CGT 880 ACGT MseI 1235 TTAA T^TAA 1318 TTAA MslI 1135 CACAGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2347 CACTTAGATG NcoI 153 CCATGG C^CATGG 2291 CCATGG PflFI 1673 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2246 GACTGGGTC PshAI 2430 GACTGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2971 GACCTCAGTC SacI 3066 GAGCTC GAGCT^C 3205 GAGCTC SmlI 1417 CTCAAG C^T[CT][GA]AG 2362 CTTGAG Tru9I 1235 TTAA T^TAA 1318 TTAA Tth111I 1673 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2246 GACTGGGTC ######################################### # 3 OR MORE CUT ENZYMES ( 97 enzymes) # ######################################### AfaI AflIII AluI AlwI AlwNI AspHI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspLU11I BspMI BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I CelII Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII