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# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspI AviII AvrII BbeI BbrPI
BbsI BciVI BfrI BlnI BpuAI
BsaAI BsaBI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspEI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I DraI
DrdI EagI EclXI Eco47III Eco52I
EcoO65I EcoT22I FseI FspI HaeII
HincII HindII HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MunI NarI NdeI NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI SacII
SalI SbfI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AcyI 43 GGCGTC G[GA]^CG[CT]C
AflIII 2124 ACATGT A^C[GA][CT]GT
AhdI 758 GACGGACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1685 CCTAAGG CC^T[ACGT]AGG
Asp700I 2110 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 758 GACGGACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1134 TGGCCA TGG^CCA
BamHI 1313 GGATCC G^GATCC
BbvCI 500 GCTGAGG CCTCAGC(-5/-2)
BcgI 2296 GCACACAGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 942 TGATCA T^GATCA
BglI 2165 GCCTGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1768 AGATCT A^GATCT
BlpI 1570 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1570 GCTGAGC GC^T[ACGT]AGC
BsaHI 43 GGCGTC G[GA]^CG[CT]C
BsgI 380 CTGCAC GTGCAG(16/14)
BspDI 1592 ATCGAT AT^CGAT
BspHI 2028 TCATGA T^CATGA
BspLU11I 2124 ACATGT A^CATGT
BsrFI 147 GCCGGC [GA]^CCGG[CT]
BssSI 2401 CACGAG CACGAG(-5/-1)
BstXI 1863 CCAAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1685 CCTAAGG CC^T[ACGT]AGG
CelII 1570 GCTGAGC GC^T[ACGT]AGC
Cfr10I 147 GCCGGC [GA]^CCGG[CT]
ClaI 1592 ATCGAT AT^CGAT
CpoI 760 CGGACCG CG^G[AT]CCG
DraIII 1934 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 758 GACGGACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1685 CCTAAGG CC^T[ACGT]AGG
EcoRI 1435 GAATTC G^AATTC
EcoRV 2024 GATATC GAT^ATC
FbaI 942 TGATCA T^GATCA
Hin1I 43 GGCGTC G[GA]^CG[CT]C
MluNI 1134 TGGCCA TGG^CCA
MscI 1134 TGGCCA TGG^CCA
MseI 441 TTAA T^TAA
NaeI 147 GCCGGC GCC^GGC
NgoMIV 147 GCCGGC G^CCGGC
PaeR7I 177 CTCGAG C^TCGAG
PflMI 1266 CCAGCTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 2028 TCATGA T^CATGA
RsrII 760 CGGACCG CG^G[AT]CCG
SacI 244 GAGCTC GAGCT^C
SapI 1345 GAAGAGC GCTCTTC(1/4)
ScaI 2112 AGTACT AGT^ACT
SfcI 2380 CTACAG C^T[GA][CT]AG
SmaI 117 CCCGGG CCC^GGG
SphI 1846 GCATGC GCATG^C
Tru9I 441 TTAA T^TAA
Van91I 1266 CCAGCTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1285 CCATAAGACACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 177 CTCGAG C^TCGAG
XmaCI 117 CCCGGG C^CCGGG
XmaI 117 CCCGGG C^CCGGG
XmnI 2110 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AspHI 244 GAGCTC G[AT]GC[AT]^C
2398 GAGCAC
BanI 34 GGTGCC G^G[CT][GA]CC
2251 GGTGCC
BsiHKAI 244 GAGCTC G[AT]GC[AT]^C
2398 GAGCAC
BspMI 31 GCAGGT ACCTGC(4/8)
159 GCAGGT
BsrBI 469 CCGCTC CCGCTC(-3/-3)
50 GAGCGG
BsrDI 1411 CATTGC GCAATG(2/0)
129 GCAATG
DraII 371 GGGTCCC [GA]G^G[ACGT]CC[CT]
1643 GGGACCT
EaeI 149 CGGCCA [CT]^GGCC[GA]
1134 TGGCCA
EcoNI 1576 CCTGGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1744 CCTATGGGAGG
EcoO109I 371 GGGTCCC [GA]G^G[ACGT]CC[CT]
1643 GGGACCT
FauI 231 CCCGC CCCGC(4/6)
216 GCGGG
MaeII 83 ACGT A^CGT
362 ACGT
MspA1I 831 CAGCTG C[AC]G^C[GT]G
1952 CAGCTG
NcoI 39 CCATGG C^CATGG
1250 CCATGG
PpuMI 371 GGGTCCC [GA]G^G[AT]CC[CT]
1643 GGGACCT
PvuII 831 CAGCTG CAG^CTG
1952 CAGCTG
SexAI 1294 ACCTGGT A^CC[AT]GGT
1935 ACCAGGT
StuI 560 AGGCCT AGG^CCT
1402 AGGCCT
XbaI 400 TCTAGA T^CTAGA
1609 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AvaI AvaII
BanII BbvI BcnI BfaI BmyI
BpmI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DsaI EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MslI MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SmlI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII