################################## # NO CUT ENZYMES ( 98 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AgeI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspI AviII AvrII BbeI BbrPI BbsI BciVI BfrI BlnI BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspEI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I DraI DrdI EagI EclXI Eco47III Eco52I EcoO65I EcoT22I FseI FspI HaeII HincII HindII HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI SacII SalI SbfI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI Tth111I ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AcyI 43 GGCGTC G[GA]^CG[CT]C AflIII 2124 ACATGT A^C[GA][CT]GT AhdI 758 GACGGACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 1685 CCTAAGG CC^T[ACGT]AGG Asp700I 2110 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 758 GACGGACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 1134 TGGCCA TGG^CCA BamHI 1313 GGATCC G^GATCC BbvCI 500 GCTGAGG CCTCAGC(-5/-2) BcgI 2296 GCACACAGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 942 TGATCA T^GATCA BglI 2165 GCCTGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1768 AGATCT A^GATCT BlpI 1570 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1570 GCTGAGC GC^T[ACGT]AGC BsaHI 43 GGCGTC G[GA]^CG[CT]C BsgI 380 CTGCAC GTGCAG(16/14) BspDI 1592 ATCGAT AT^CGAT BspHI 2028 TCATGA T^CATGA BspLU11I 2124 ACATGT A^CATGT BsrFI 147 GCCGGC [GA]^CCGG[CT] BssSI 2401 CACGAG CACGAG(-5/-1) BstXI 1863 CCAAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1685 CCTAAGG CC^T[ACGT]AGG CelII 1570 GCTGAGC GC^T[ACGT]AGC Cfr10I 147 GCCGGC [GA]^CCGG[CT] ClaI 1592 ATCGAT AT^CGAT CpoI 760 CGGACCG CG^G[AT]CCG DraIII 1934 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 758 GACGGACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1685 CCTAAGG CC^T[ACGT]AGG EcoRI 1435 GAATTC G^AATTC EcoRV 2024 GATATC GAT^ATC FbaI 942 TGATCA T^GATCA Hin1I 43 GGCGTC G[GA]^CG[CT]C MluNI 1134 TGGCCA TGG^CCA MscI 1134 TGGCCA TGG^CCA MseI 441 TTAA T^TAA NaeI 147 GCCGGC GCC^GGC NgoMIV 147 GCCGGC G^CCGGC PaeR7I 177 CTCGAG C^TCGAG PflMI 1266 CCAGCTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 2028 TCATGA T^CATGA RsrII 760 CGGACCG CG^G[AT]CCG SacI 244 GAGCTC GAGCT^C SapI 1345 GAAGAGC GCTCTTC(1/4) ScaI 2112 AGTACT AGT^ACT SfcI 2380 CTACAG C^T[GA][CT]AG SmaI 117 CCCGGG CCC^GGG SphI 1846 GCATGC GCATG^C Tru9I 441 TTAA T^TAA Van91I 1266 CCAGCTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1285 CCATAAGACACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 177 CTCGAG C^TCGAG XmaCI 117 CCCGGG C^CCGGG XmaI 117 CCCGGG C^CCGGG XmnI 2110 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AspHI 244 GAGCTC G[AT]GC[AT]^C 2398 GAGCAC BanI 34 GGTGCC G^G[CT][GA]CC 2251 GGTGCC BsiHKAI 244 GAGCTC G[AT]GC[AT]^C 2398 GAGCAC BspMI 31 GCAGGT ACCTGC(4/8) 159 GCAGGT BsrBI 469 CCGCTC CCGCTC(-3/-3) 50 GAGCGG BsrDI 1411 CATTGC GCAATG(2/0) 129 GCAATG DraII 371 GGGTCCC [GA]G^G[ACGT]CC[CT] 1643 GGGACCT EaeI 149 CGGCCA [CT]^GGCC[GA] 1134 TGGCCA EcoNI 1576 CCTGGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1744 CCTATGGGAGG EcoO109I 371 GGGTCCC [GA]G^G[ACGT]CC[CT] 1643 GGGACCT FauI 231 CCCGC CCCGC(4/6) 216 GCGGG MaeII 83 ACGT A^CGT 362 ACGT MspA1I 831 CAGCTG C[AC]G^C[GT]G 1952 CAGCTG NcoI 39 CCATGG C^CATGG 1250 CCATGG PpuMI 371 GGGTCCC [GA]G^G[AT]CC[CT] 1643 GGGACCT PvuII 831 CAGCTG CAG^CTG 1952 CAGCTG SexAI 1294 ACCTGGT A^CC[AT]GGT 1935 ACCAGGT StuI 560 AGGCCT AGG^CCT 1402 AGGCCT XbaI 400 TCTAGA T^CTAGA 1609 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 84 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI ApoI AvaI AvaII BanII BbvI BcnI BfaI BmyI BpmI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DsaI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MslI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SmlI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I XhoII