##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AccIII AgeI Aor51HI AscI AvaI
AviII AvrII BalI BbeI BbrPI
BbvCI BciVI BglI BlnI BsaI
BseAI BsiWI BsmI BsoBI Bsp1407I
BspDI BspEI BsrBI BsrGI BssHII
BstBI BstEII BstPI ClaI CpoI
DraI DrdI EagI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
FseI FspI HincII HindII HpaI
KasI KspI MfeI MluI MluNI
MroI MscI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PpuMI PshAI PvuI
PvuII RsrII SacII SalI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI SspBI SspI SwaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 223 GACGTC GACGT^C
AccII 1357 CGCG CG^CG
AcsI 918 GAATTT [GA]^AATT[CT]
Alw44I 1390 GTGCAC G^TGCAC
AocI 2061 CCTGAGG CC^T[ACGT]AGG
ApaI 1541 GGGCCC GGGCC^C
ApaLI 1390 GTGCAC G^TGCAC
ApoI 918 GAATTT [GA]^AATT[CT]
AseI 1405 ATTAAT AT^TAAT
AsnI 1405 ATTAAT AT^TAAT
Asp700I 610 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1056 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2439 GGATCC G^GATCC
BbsI 301 GAAGAC GAAGAC(2/6)
BcgI 721 CGAAGCATCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 968 TGATCA T^GATCA
BglII 1922 AGATCT A^GATCT
BpuAI 301 GAAGAC GAAGAC(2/6)
BsaAI 712 TACGTG [CT]AC^GT[GA]
BsaBI 1560 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 467 TCCGGT [AT]^CCGG[AT]
BsiEI 648 CGGTCG CG[GA][CT]^CG
BsmBI 1054 GAGACG CGTCTC(1/5)
BspHI 243 TCATGA T^CATGA
BstUI 1357 CGCG CG^CG
Bsu36I 2061 CCTGAGG CC^T[ACGT]AGG
DraIII 767 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 663 TGGCCG [CT]^GGCC[GA]
Eco81I 2061 CCTGAGG CC^T[ACGT]AGG
EcoT22I 1456 ATGCAT ATGCA^T
FauI 2846 CCCGC CCCGC(4/6)
FbaI 968 TGATCA T^GATCA
HindIII 1726 AAGCTT A^AGCTT
MamI 1560 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 1357 CGCG CG^CG
NsiI 1456 ATGCAT ATGCA^T
PflFI 1056 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 1405 ATTAAT AT^TAAT
RcaI 243 TCATGA T^CATGA
SacI 1600 GAGCTC GAGCT^C
SbfI 2758 CCTGCAGG CCTGCA^GG
Sse8387I 2758 CCTGCAGG CCTGCA^GG
Tth111I 1056 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1128 TCTAGA T^CTAGA
XcmI 2340 CCATGCAAATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 610 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
Acc65I 2764 GGTACC G^GTACC
2965 GGTACC
AclI 752 AACGTT AA^CGTT
1647 AACGTT
AflII 157 CTTAAG C^TTAAG
760 CTTAAG
AflIII 1330 ACATGT A^C[GA][CT]GT
1661 ACATGT
AhdI 684 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1582 GACATGCAGTC
Asp718I 2764 GGTACC G^GTACC
2965 GGTACC
AspEI 684 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1582 GACATGCAGTC
BcnI 598 CCGGG CC^[GC]GG
2497 CCCGG
BfrI 157 CTTAAG C^TTAAG
760 CTTAAG
BlpI 1119 GCTCAGC GC^T[ACGT]AGC
1626 GCTGAGC
Bpu1102I 1119 GCTCAGC GC^T[ACGT]AGC
1626 GCTGAGC
BspLU11I 1330 ACATGT A^CATGT
1661 ACATGT
BsrFI 115 ACCGGC [GA]^CCGG[CT]
2567 ACCGGC
BssSI 2018 CACGAG CACGAG(-5/-1)
2432 CTCGTG
Bst1107I 2397 GTATAC GTA^TAC
2563 GTATAC
BstXI 435 CCAGTCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1969 CCACTGTCATGG
BstZ17I 2397 GTATAC GTA^TAC
2563 GTATAC
CelII 1119 GCTCAGC GC^T[ACGT]AGC
1626 GCTGAGC
Cfr10I 115 ACCGGC [GA]^CCGG[CT]
2567 ACCGGC
DsaI 2387 CCATGG C^C[GA][CT]GG
2768 CCATGG
Eam1105I 684 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1582 GACATGCAGTC
KpnI 2764 GGTACC GGTAC^C
2965 GGTACC
NciI 598 CCGGG CC^[GC]GG
2497 CCCGG
NcoI 2387 CCATGG C^CATGG
2768 CCATGG
PflMI 486 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2893 CCAACCTATGG
Psp1406I 752 AACGTT AA^CGTT
1647 AACGTT
ScaI 153 AGTACT AGT^ACT
1424 AGTACT
SexAI 1484 ACCTGGT A^CC[AT]GGT
1715 ACCTGGT
StuI 161 AGGCCT AGG^CCT
2065 AGGCCT
Tsp509I 919 AATT ^AATT
1408 AATT
Van91I 486 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2893 CCAACCTATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccI AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaII BanI
BanII BbvI BfaI BmyI BpmI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrDI BsrI BssKI BstAPI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
PstI RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
TspRI TthHB8I XhoII