################################## # NO CUT ENZYMES ( 88 enzymes) # ################################## AccIII AgeI Aor51HI AscI AvaI AviII AvrII BalI BbeI BbrPI BbvCI BciVI BglI BlnI BsaI BseAI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BsrBI BsrGI BssHII BstBI BstEII BstPI ClaI CpoI DraI DrdI EagI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV FseI FspI HincII HindII HpaI KasI KspI MfeI MluI MluNI MroI MscI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI PvuI PvuII RsrII SacII SalI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI SspBI SspI SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AatII 223 GACGTC GACGT^C AccII 1357 CGCG CG^CG AcsI 918 GAATTT [GA]^AATT[CT] Alw44I 1390 GTGCAC G^TGCAC AocI 2061 CCTGAGG CC^T[ACGT]AGG ApaI 1541 GGGCCC GGGCC^C ApaLI 1390 GTGCAC G^TGCAC ApoI 918 GAATTT [GA]^AATT[CT] AseI 1405 ATTAAT AT^TAAT AsnI 1405 ATTAAT AT^TAAT Asp700I 610 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 1056 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 2439 GGATCC G^GATCC BbsI 301 GAAGAC GAAGAC(2/6) BcgI 721 CGAAGCATCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 968 TGATCA T^GATCA BglII 1922 AGATCT A^GATCT BpuAI 301 GAAGAC GAAGAC(2/6) BsaAI 712 TACGTG [CT]AC^GT[GA] BsaBI 1560 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 467 TCCGGT [AT]^CCGG[AT] BsiEI 648 CGGTCG CG[GA][CT]^CG BsmBI 1054 GAGACG CGTCTC(1/5) BspHI 243 TCATGA T^CATGA BstUI 1357 CGCG CG^CG Bsu36I 2061 CCTGAGG CC^T[ACGT]AGG DraIII 767 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 663 TGGCCG [CT]^GGCC[GA] Eco81I 2061 CCTGAGG CC^T[ACGT]AGG EcoT22I 1456 ATGCAT ATGCA^T FauI 2846 CCCGC CCCGC(4/6) FbaI 968 TGATCA T^GATCA HindIII 1726 AAGCTT A^AGCTT MamI 1560 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MvnI 1357 CGCG CG^CG NsiI 1456 ATGCAT ATGCA^T PflFI 1056 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PshBI 1405 ATTAAT AT^TAAT RcaI 243 TCATGA T^CATGA SacI 1600 GAGCTC GAGCT^C SbfI 2758 CCTGCAGG CCTGCA^GG Sse8387I 2758 CCTGCAGG CCTGCA^GG Tth111I 1056 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 1128 TCTAGA T^CTAGA XcmI 2340 CCATGCAAATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 610 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### Acc65I 2764 GGTACC G^GTACC 2965 GGTACC AclI 752 AACGTT AA^CGTT 1647 AACGTT AflII 157 CTTAAG C^TTAAG 760 CTTAAG AflIII 1330 ACATGT A^C[GA][CT]GT 1661 ACATGT AhdI 684 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1582 GACATGCAGTC Asp718I 2764 GGTACC G^GTACC 2965 GGTACC AspEI 684 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1582 GACATGCAGTC BcnI 598 CCGGG CC^[GC]GG 2497 CCCGG BfrI 157 CTTAAG C^TTAAG 760 CTTAAG BlpI 1119 GCTCAGC GC^T[ACGT]AGC 1626 GCTGAGC Bpu1102I 1119 GCTCAGC GC^T[ACGT]AGC 1626 GCTGAGC BspLU11I 1330 ACATGT A^CATGT 1661 ACATGT BsrFI 115 ACCGGC [GA]^CCGG[CT] 2567 ACCGGC BssSI 2018 CACGAG CACGAG(-5/-1) 2432 CTCGTG Bst1107I 2397 GTATAC GTA^TAC 2563 GTATAC BstXI 435 CCAGTCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1969 CCACTGTCATGG BstZ17I 2397 GTATAC GTA^TAC 2563 GTATAC CelII 1119 GCTCAGC GC^T[ACGT]AGC 1626 GCTGAGC Cfr10I 115 ACCGGC [GA]^CCGG[CT] 2567 ACCGGC DsaI 2387 CCATGG C^C[GA][CT]GG 2768 CCATGG Eam1105I 684 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1582 GACATGCAGTC KpnI 2764 GGTACC GGTAC^C 2965 GGTACC NciI 598 CCGGG CC^[GC]GG 2497 CCCGG NcoI 2387 CCATGG C^CATGG 2768 CCATGG PflMI 486 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2893 CCAACCTATGG Psp1406I 752 AACGTT AA^CGTT 1647 AACGTT ScaI 153 AGTACT AGT^ACT 1424 AGTACT SexAI 1484 ACCTGGT A^CC[AT]GGT 1715 ACCTGGT StuI 161 AGGCCT AGG^CCT 2065 AGGCCT Tsp509I 919 AATT ^AATT 1408 AATT Van91I 486 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2893 CCAACCTATGG ######################################### # 3 OR MORE CUT ENZYMES ( 93 enzymes) # ######################################### AccI AciI AcyI AfaI AluI AlwI AlwNI AspHI AvaII BanI BanII BbvI BfaI BmyI BpmI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PleI PstI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I TspRI TthHB8I XhoII