##################################
# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII AccII AclI AcsI AcyI
AflII AgeI AhdI ApoI AscI
AseI AsnI Asp700I AspEI AspI
AviII AvrII BbeI BbsI BbvCI
BciVI BclI BfrI BglII BlnI
BpuAI BsaBI BsaHI BsiWI Bsp1407I
BspDI BspHI BspLU11I BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstUI BstZ17I ClaI CpoI DraI
DrdI Eam1105I EcoRI EcoRV EcoT22I
FbaI FseI FspI Hin1I HincII
HindII HindIII HpaI KasI KspI
MamI MfeI MluI MseI MunI
MvnI NarI NdeI NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SfiI SfoI SfuI SgrAI
SmaI SnaBI SspBI SspI SwaI
Tru9I Tsp509I Tth111I XbaI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
Acc65I 1308 GGTACC G^GTACC
AccI 1926 GTCTAC GT^[AC][GT]AC
AccIII 675 TCCGGA T^CCGGA
AflIII 502 ACGTGT A^C[GA][CT]GT
Alw44I 942 GTGCAC G^TGCAC
Aor51HI 2248 AGCGCT AGC^GCT
ApaI 1439 GGGCCC GGGCC^C
ApaLI 942 GTGCAC G^TGCAC
Asp718I 1308 GGTACC G^GTACC
AvaI 1649 CTCGAG C^[CT]CG[GA]G
BlpI 2255 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2255 GCTCAGC GC^T[ACGT]AGC
BsaWI 675 TCCGGA [AT]^CCGG[AT]
BseAI 675 TCCGGA T^CCGGA
BsiEI 642 CGGCCG CG[GA][CT]^CG
BsmBI 1791 CGTCTC CGTCTC(1/5)
BsoBI 1649 CTCGAG C^[CT]CG[GA]G
BspEI 675 TCCGGA T^CCGGA
BstAPI 1334 GCATCGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 494 GGTCACC G^GT[ACGT]ACC
BstPI 494 GGTCACC G^GT[ACGT]ACC
CelII 2255 GCTCAGC GC^T[ACGT]AGC
EagI 642 CGGCCG C^GGCCG
EclXI 642 CGGCCG C^GGCCG
Eco47III 2248 AGCGCT AGC^GCT
Eco52I 642 CGGCCG C^GGCCG
EcoNI 2388 CCTGGAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 494 GGTCACC G^GT[ACGT]ACC
HaeII 2248 AGCGCT [GA]GCGC^[CT]
KpnI 1308 GGTACC GGTAC^C
MroI 675 TCCGGA T^CCGGA
PaeR7I 1649 CTCGAG C^TCGAG
ScaI 308 AGTACT AGT^ACT
SexAI 2016 ACCTGGT A^CC[AT]GGT
SmlI 1649 CTCGAG C^T[CT][GA]AG
SpeI 1996 ACTAGT A^CTAGT
SphI 629 GCATGC GCATG^C
StuI 1387 AGGCCT AGG^CCT
XhoI 1649 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AocI 1723 CCTGAGG CC^T[ACGT]AGG
2125 CCTGAGG
BamHI 88 GGATCC G^GATCC
2184 GGATCC
BbrPI 350 CACGTG CAC^GTG
501 CACGTG
BcgI 706 CGAGGCCAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1623 CGAAGCTCATGC
BsaI 331 GAGACC GGTCTC(1/5)
2047 GAGACC
BsmI 181 GAATGC GAATGC(1/-1)
837 GAATGC
BstXI 546 CCAGGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2321 CCAGAGGTCTGG
Bsu36I 1723 CCTGAGG CC^T[ACGT]AGG
2125 CCTGAGG
CfoI 2220 GCGC GCG^C
2249 GCGC
DraIII 536 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
1243 CACCCTGTG
Eco81I 1723 CCTGAGG CC^T[ACGT]AGG
2125 CCTGAGG
HgaI 2371 GACGC GACGC(5/10)
650 GCGTC
HhaI 2220 GCGC GCG^C
2249 GCGC
HinP1I 2220 GCGC G^CGC
2249 GCGC
NaeI 965 GCCGGC GCC^GGC
1567 GCCGGC
NgoMIV 965 GCCGGC G^CCGGC
1567 GCCGGC
NspI 629 GCATGC [GA]CATG^[CT]
844 ACATGC
PmaCI 350 CACGTG CAC^GTG
501 CACGTG
PmlI 350 CACGTG CAC^GTG
501 CACGTG
SbfI 215 CCTGCAGG CCTGCA^GG
919 CCTGCAGG
Sse8387I 215 CCTGCAGG CCTGCA^GG
919 CCTGCAGG
TaqI 1553 TCGA T^CGA
1650 TCGA
TthHB8I 1553 TCGA T^CGA
1650 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaII BalI BanI BanII
BbvI BcnI BfaI BglI BmyI
BpmI BsaAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TfiI TseI Tsp45I
TspRI Van91I XcmI XhoII