################################## # NO CUT ENZYMES ( 102 enzymes) # ################################## AatII AccII AclI AcsI AcyI AflII AgeI AhdI ApoI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BbeI BbsI BbvCI BciVI BclI BfrI BglII BlnI BpuAI BsaBI BsaHI BsiWI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstUI BstZ17I ClaI CpoI DraI DrdI Eam1105I EcoRI EcoRV EcoT22I FbaI FseI FspI Hin1I HincII HindII HindIII HpaI KasI KspI MamI MfeI MluI MseI MunI MvnI NarI NdeI NheI NotI NruI NsiI NspV PacI PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SfiI SfoI SfuI SgrAI SmaI SnaBI SspBI SspI SwaI Tru9I Tsp509I Tth111I XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### Acc65I 1308 GGTACC G^GTACC AccI 1926 GTCTAC GT^[AC][GT]AC AccIII 675 TCCGGA T^CCGGA AflIII 502 ACGTGT A^C[GA][CT]GT Alw44I 942 GTGCAC G^TGCAC Aor51HI 2248 AGCGCT AGC^GCT ApaI 1439 GGGCCC GGGCC^C ApaLI 942 GTGCAC G^TGCAC Asp718I 1308 GGTACC G^GTACC AvaI 1649 CTCGAG C^[CT]CG[GA]G BlpI 2255 GCTCAGC GC^T[ACGT]AGC Bpu1102I 2255 GCTCAGC GC^T[ACGT]AGC BsaWI 675 TCCGGA [AT]^CCGG[AT] BseAI 675 TCCGGA T^CCGGA BsiEI 642 CGGCCG CG[GA][CT]^CG BsmBI 1791 CGTCTC CGTCTC(1/5) BsoBI 1649 CTCGAG C^[CT]CG[GA]G BspEI 675 TCCGGA T^CCGGA BstAPI 1334 GCATCGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 494 GGTCACC G^GT[ACGT]ACC BstPI 494 GGTCACC G^GT[ACGT]ACC CelII 2255 GCTCAGC GC^T[ACGT]AGC EagI 642 CGGCCG C^GGCCG EclXI 642 CGGCCG C^GGCCG Eco47III 2248 AGCGCT AGC^GCT Eco52I 642 CGGCCG C^GGCCG EcoNI 2388 CCTGGAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 494 GGTCACC G^GT[ACGT]ACC HaeII 2248 AGCGCT [GA]GCGC^[CT] KpnI 1308 GGTACC GGTAC^C MroI 675 TCCGGA T^CCGGA PaeR7I 1649 CTCGAG C^TCGAG ScaI 308 AGTACT AGT^ACT SexAI 2016 ACCTGGT A^CC[AT]GGT SmlI 1649 CTCGAG C^T[CT][GA]AG SpeI 1996 ACTAGT A^CTAGT SphI 629 GCATGC GCATG^C StuI 1387 AGGCCT AGG^CCT XhoI 1649 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AocI 1723 CCTGAGG CC^T[ACGT]AGG 2125 CCTGAGG BamHI 88 GGATCC G^GATCC 2184 GGATCC BbrPI 350 CACGTG CAC^GTG 501 CACGTG BcgI 706 CGAGGCCAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1623 CGAAGCTCATGC BsaI 331 GAGACC GGTCTC(1/5) 2047 GAGACC BsmI 181 GAATGC GAATGC(1/-1) 837 GAATGC BstXI 546 CCAGGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2321 CCAGAGGTCTGG Bsu36I 1723 CCTGAGG CC^T[ACGT]AGG 2125 CCTGAGG CfoI 2220 GCGC GCG^C 2249 GCGC DraIII 536 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG 1243 CACCCTGTG Eco81I 1723 CCTGAGG CC^T[ACGT]AGG 2125 CCTGAGG HgaI 2371 GACGC GACGC(5/10) 650 GCGTC HhaI 2220 GCGC GCG^C 2249 GCGC HinP1I 2220 GCGC G^CGC 2249 GCGC NaeI 965 GCCGGC GCC^GGC 1567 GCCGGC NgoMIV 965 GCCGGC G^CCGGC 1567 GCCGGC NspI 629 GCATGC [GA]CATG^[CT] 844 ACATGC PmaCI 350 CACGTG CAC^GTG 501 CACGTG PmlI 350 CACGTG CAC^GTG 501 CACGTG SbfI 215 CCTGCAGG CCTGCA^GG 919 CCTGCAGG Sse8387I 215 CCTGCAGG CCTGCA^GG 919 CCTGCAGG TaqI 1553 TCGA T^CGA 1650 TCGA TthHB8I 1553 TCGA T^CGA 1650 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 94 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AspHI AvaII BalI BanI BanII BbvI BcnI BfaI BglI BmyI BpmI BsaAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TfiI TseI Tsp45I TspRI Van91I XcmI XhoII