##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII AccI AclI AcsI AcyI
AflII AflIII Aor51HI ApoI AscI
AseI AsnI AspI AvrII BbeI
BclI BfrI BlnI BsaBI BsaHI
BsaI BsiEI BsiWI Bsp1407I BspHI
BspLU11I BsrGI BssHII Bst1107I BstEII
BstPI BstZ17I CpoI DraI DrdI
EagI EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HgaI Hin1I HincII HindII
HpaI KasI KspI MamI MfeI
MluI MunI NaeI NarI NcoI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflFI PmeI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SbfI SexAI SfoI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AccIII 3611 TCCGGA T^CCGGA
AgeI 1090 ACCGGT A^CCGGT
AhdI 1887 GACGGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3357 GTGCAC G^TGCAC
ApaLI 3357 GTGCAC G^TGCAC
AspEI 1887 GACGGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 930 TGCGCA TGC^GCA
BamHI 319 GGATCC G^GATCC
BciVI 2599 GTATCC GTATCC(6/5)
BseAI 3611 TCCGGA T^CCGGA
BsmBI 1378 CGTCTC CGTCTC(1/5)
BsmI 1489 GCATTC GAATGC(1/-1)
BspDI 1390 ATCGAT AT^CGAT
BspEI 3611 TCCGGA T^CCGGA
BsrBI 2108 GAGCGG CCGCTC(-3/-3)
BsrDI 2033 GCAATG GCAATG(2/0)
BssSI 469 CTCGTG CACGAG(-5/-1)
BstBI 888 TTCGAA TT^CGAA
BstXI 1155 CCACACCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 1390 ATCGAT AT^CGAT
DraIII 2527 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1887 GACGGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 930 TGCGCA TGC^GCA
MroI 3611 TCCGGA T^CCGGA
NdeI 1688 CATATG CA^TATG
NspI 2552 GCATGC [GA]CATG^[CT]
NspV 888 TTCGAA TT^CGAA
PinAI 1090 ACCGGT A^CCGGT
PshAI 2806 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfuI 888 TTCGAA TT^CGAA
SgrAI 1089 CACCGGTG C[GA]^CCGG[CT]G
SphI 2552 GCATGC GCATG^C
StuI 2153 AGGCCT AGG^CCT
XbaI 1123 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
Acc65I 1342 GGTACC G^GTACC
2655 GGTACC
AccII 2589 CGCG CG^CG
3175 CGCG
Asp700I 1976 GAAACCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2019 GAAAGCTTTC
Asp718I 1342 GGTACC G^GTACC
2655 GGTACC
BbrPI 234 CACGTG CAC^GTG
878 CACGTG
BbvCI 504 CCTCAGC CCTCAGC(-5/-2)
1590 CCTCAGC
BcgI 3 CGATGCCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
428 GCAGCCCCTTCG
BglII 2283 AGATCT A^GATCT
2849 AGATCT
BlpI 2136 GCTCAGC GC^T[ACGT]AGC
3298 GCTAAGC
Bpu1102I 2136 GCTCAGC GC^T[ACGT]AGC
3298 GCTAAGC
BsaWI 1090 ACCGGT [AT]^CCGG[AT]
3611 TCCGGA
BstUI 2589 CGCG CG^CG
3175 CGCG
CelII 2136 GCTCAGC GC^T[ACGT]AGC
3298 GCTAAGC
EcoT14I 35 CCAAGG C^C[AT][AT]GG
1866 CCAAGG
HindIII 2021 AAGCTT A^AGCTT
2230 AAGCTT
KpnI 1342 GGTACC GGTAC^C
2655 GGTACC
MvnI 2589 CGCG CG^CG
3175 CGCG
PflMI 2526 CCACTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3492 CCAGTACTTGG
PmaCI 234 CACGTG CAC^GTG
878 CACGTG
PmlI 234 CACGTG CAC^GTG
878 CACGTG
ScaI 143 AGTACT AGT^ACT
3494 AGTACT
SfiI 641 GGCCTCCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
875 GGCCACGTGGGCC
SmaI 2648 CCCGGG CCC^GGG
2857 CCCGGG
StyI 35 CCAAGG C^C[AT][AT]GG
1866 CCAAGG
Tsp509I 2329 AATT ^AATT
2388 AATT
Van91I 2526 CCACTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3492 CCAGTACTTGG
XcmI 1383 CCATCCCATCGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2937 CCAGGGTGGGCCTGG
XmaCI 2648 CCCGGG C^CCGGG
2857 CCCGGG
XmaI 2648 CCCGGG C^CCGGG
2857 CCCGGG
XmnI 1976 GAAACCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2019 GAAAGCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI ApaI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PstI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI TaqI TfiI
Tru9I TseI Tsp45I TspRI TthHB8I
XhoII