################################## # NO CUT ENZYMES ( 88 enzymes) # ################################## AatII AccI AclI AcsI AcyI AflII AflIII Aor51HI ApoI AscI AseI AsnI AspI AvrII BbeI BclI BfrI BlnI BsaBI BsaHI BsaI BsiEI BsiWI Bsp1407I BspHI BspLU11I BsrGI BssHII Bst1107I BstEII BstPI BstZ17I CpoI DraI DrdI EagI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HgaI Hin1I HincII HindII HpaI KasI KspI MamI MfeI MluI MunI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PmeI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI SexAI SfoI SnaBI SpeI Sse8387I SspBI SspI SwaI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AccIII 3611 TCCGGA T^CCGGA AgeI 1090 ACCGGT A^CCGGT AhdI 1887 GACGGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 3357 GTGCAC G^TGCAC ApaLI 3357 GTGCAC G^TGCAC AspEI 1887 GACGGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 930 TGCGCA TGC^GCA BamHI 319 GGATCC G^GATCC BciVI 2599 GTATCC GTATCC(6/5) BseAI 3611 TCCGGA T^CCGGA BsmBI 1378 CGTCTC CGTCTC(1/5) BsmI 1489 GCATTC GAATGC(1/-1) BspDI 1390 ATCGAT AT^CGAT BspEI 3611 TCCGGA T^CCGGA BsrBI 2108 GAGCGG CCGCTC(-3/-3) BsrDI 2033 GCAATG GCAATG(2/0) BssSI 469 CTCGTG CACGAG(-5/-1) BstBI 888 TTCGAA TT^CGAA BstXI 1155 CCACACCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ClaI 1390 ATCGAT AT^CGAT DraIII 2527 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 1887 GACGGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC FspI 930 TGCGCA TGC^GCA MroI 3611 TCCGGA T^CCGGA NdeI 1688 CATATG CA^TATG NspI 2552 GCATGC [GA]CATG^[CT] NspV 888 TTCGAA TT^CGAA PinAI 1090 ACCGGT A^CCGGT PshAI 2806 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SfuI 888 TTCGAA TT^CGAA SgrAI 1089 CACCGGTG C[GA]^CCGG[CT]G SphI 2552 GCATGC GCATG^C StuI 2153 AGGCCT AGG^CCT XbaI 1123 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### Acc65I 1342 GGTACC G^GTACC 2655 GGTACC AccII 2589 CGCG CG^CG 3175 CGCG Asp700I 1976 GAAACCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2019 GAAAGCTTTC Asp718I 1342 GGTACC G^GTACC 2655 GGTACC BbrPI 234 CACGTG CAC^GTG 878 CACGTG BbvCI 504 CCTCAGC CCTCAGC(-5/-2) 1590 CCTCAGC BcgI 3 CGATGCCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 428 GCAGCCCCTTCG BglII 2283 AGATCT A^GATCT 2849 AGATCT BlpI 2136 GCTCAGC GC^T[ACGT]AGC 3298 GCTAAGC Bpu1102I 2136 GCTCAGC GC^T[ACGT]AGC 3298 GCTAAGC BsaWI 1090 ACCGGT [AT]^CCGG[AT] 3611 TCCGGA BstUI 2589 CGCG CG^CG 3175 CGCG CelII 2136 GCTCAGC GC^T[ACGT]AGC 3298 GCTAAGC EcoT14I 35 CCAAGG C^C[AT][AT]GG 1866 CCAAGG HindIII 2021 AAGCTT A^AGCTT 2230 AAGCTT KpnI 1342 GGTACC GGTAC^C 2655 GGTACC MvnI 2589 CGCG CG^CG 3175 CGCG PflMI 2526 CCACTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3492 CCAGTACTTGG PmaCI 234 CACGTG CAC^GTG 878 CACGTG PmlI 234 CACGTG CAC^GTG 878 CACGTG ScaI 143 AGTACT AGT^ACT 3494 AGTACT SfiI 641 GGCCTCCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 875 GGCCACGTGGGCC SmaI 2648 CCCGGG CCC^GGG 2857 CCCGGG StyI 35 CCAAGG C^C[AT][AT]GG 1866 CCAAGG Tsp509I 2329 AATT ^AATT 2388 AATT Van91I 2526 CCACTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3492 CCAGTACTTGG XcmI 1383 CCATCCCATCGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2937 CCAGGGTGGGCCTGG XmaCI 2648 CCCGGG C^CCGGG 2857 CCCGGG XmaI 2648 CCCGGG C^CCGGG 2857 CCCGGG XmnI 1976 GAAACCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2019 GAAAGCTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 106 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AocI ApaI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI TfiI Tru9I TseI Tsp45I TspRI TthHB8I XhoII