##################################
# NO CUT ENZYMES ( 164 enzymes) #
##################################
AatII AccII AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AspHI
AspI AvaI AvaII AviII AvrII
BalI BamHI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaWI BseAI
BseRI BsgI BsiHKAI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII CfoI ClaI
CpoI DraI DraIII DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HhaI HinP1I HindIII HpaI
KasI Ksp632I KspI MaeIII MamI
MfeI MluI MluNI MroI MscI
MseI MunI MvnI NarI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SapI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
SspBI SspI StuI SwaI Tru9I
Tsp45I Tsp509I Tth111I Van91I XbaI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 334 GGTACC G^GTACC
AccI 1 GTCGAC GT^[AC][GT]AC
AcyI 153 GACGCC G[GA]^CG[CT]C
AlwI 54 GGATC GGATC(4/5)
AlwNI 138 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 110 GGGCCC GGGCC^C
Asp718I 334 GGTACC G^GTACC
BfaI 396 CTAG C^TAG
BsaHI 153 GACGCC G[GA]^CG[CT]C
BsaI 426 GAGACC GGTCTC(1/5)
BsiEI 115 CGGTCG CG[GA][CT]^CG
BsrFI 160 GCCGGC [GA]^CCGG[CT]
BstF5I 386 CATCC GGATG(2/0)
BstYI 54 GGATCT [GA]^GATC[CT]
Cfr10I 160 GCCGGC [GA]^CCGG[CT]
DpnII 55 GATC ^GATC
EcoNI 338 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 295 CCCGC CCCGC(4/6)
FokI 386 CATCC GGATG(9/13)
HgaI 153 GACGC GACGC(5/10)
Hin1I 153 GACGCC G[GA]^CG[CT]C
HinfI 370 GAATC G^A[ACGT]TC
KpnI 334 GGTACC GGTAC^C
MaeI 396 CTAG C^TAG
MaeII 130 ACGT A^CGT
MboI 55 GATC ^GATC
MboII 46 TCTTC GAAGA(8/7)
MflI 54 GGATCT [GA]^GATC[CT]
MslI 386 CATCCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI 200 GCTGCAGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NaeI 160 GCCGGC GCC^GGC
NdeII 55 GATC ^GATC
NgoMIV 160 GCCGGC G^CCGGC
PshAI 428 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 1 GTCGAC G^TCGAC
Sau3AI 55 GATC ^GATC
SbfI 80 CCTGCAGG CCTGCA^GG
Sse8387I 80 CCTGCAGG CCTGCA^GG
TaqI 2 TCGA T^CGA
TfiI 370 GAATC G^A[AT]TC
TspRI 328 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 2 TCGA T^CGA
XcmI 157 CCAGCCGGCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 54 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AciI 296 CCGC CCGC(-3/-1)
401 CCGC
AfaI 95 GTAC GT^AC
335 GTAC
BanI 334 GGTACC G^G[CT][GA]CC
357 GGCACC
BanII 9 GGGCTC G[GA]GC[CT]^C
110 GGGCCC
BcnI 113 CCCGG CC^[GC]GG
505 CCGGG
BsmFI 132 GTCCC GGGAC(10/14)
233 GTCCC
BspMI 83 GCAGGT ACCTGC(4/8)
344 GCAGGT
DraII 66 AGGCCCC [GA]G^G[ACGT]CC[CT]
109 AGGGCCC
EcoO109I 66 AGGCCCC [GA]G^G[ACGT]CC[CT]
109 AGGGCCC
EcoT14I 352 CCTTGG C^C[AT][AT]GG
478 CCAAGG
HincII 1 GTCGAC GT[CT]^[GA]AC
315 GTCAAC
HindII 1 GTCGAC GT[CT]^[GA]AC
315 GTCAAC
HphI 86 GGTGA GGTGA(8/7)
459 TCACC
NciI 113 CCCGG CC^[GC]GG
505 CCGGG
NlaIII 41 CATG CATG^
150 CATG
PstI 81 CTGCAG CTGCA^G
201 CTGCAG
RsaI 95 GTAC GT^AC
335 GTAC
SfaNI 392 GATGC GCATC(5/9)
385 GCATC
SmlI 282 CTCAAG C^T[CT][GA]AG
423 CTTGAG
StyI 352 CCTTGG C^C[AT][AT]GG
478 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AluI BbvI BmyI BpmI BsaJI
BsiYI BslI BsmAI Bsp1286I BssKI
BstNI Cac8I Cfr13I DdeI EcoRII
Fnu4HI HaeIII HapII HpaII ItaI
MnlI MspA1I MspI MvaI NlaIV
PvuII Sau96I ScrFI SfcI TseI