##################################
# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AflIII AhdI Alw44I AlwNI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspI AvrII
BbeI BbrPI BbvCI BciVI BclI
BfrI BglII BlnI BsaAI BsaBI
BsiEI BsiWI BsmI Bsp1407I BspLU11I
BspMI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoT14I FbaI FseI HaeII
HincII HindII HpaI KasI KpnI
MamI MfeI MluI MunI NaeI
NarI NcoI NdeI NgoMIV NotI
NruI NspV PacI PaeR7I PflFI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI PvuII RsrII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI StuI StyI SwaI
TfiI Tth111I XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AccIII 573 TCCGGA T^CCGGA
AcyI 1150 GACGCC G[GA]^CG[CT]C
AgeI 183 ACCGGT A^CCGGT
AocI 1000 CCTAAGG CC^T[ACGT]AGG
ApaI 648 GGGCCC GGGCC^C
Asp700I 1300 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 629 CTCGGG C^[CT]CG[GA]G
AviII 33 TGCGCA TGC^GCA
BamHI 250 GGATCC G^GATCC
BbsI 122 GTCTTC GAAGAC(2/6)
BcgI 192 GCACGGAGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 122 GTCTTC GAAGAC(2/6)
BsaHI 1150 GACGCC G[GA]^CG[CT]C
BsaI 272 GAGACC GGTCTC(1/5)
BseAI 573 TCCGGA T^CCGGA
BsmBI 731 GAGACG CGTCTC(1/5)
BsoBI 629 CTCGGG C^[CT]CG[GA]G
BspDI 780 ATCGAT AT^CGAT
BspEI 573 TCCGGA T^CCGGA
BsrBI 158 CCGCTC CCGCTC(-3/-3)
BsrFI 183 ACCGGT [GA]^CCGG[CT]
BstYI 250 GGATCC [GA]^GATC[CT]
Bsu36I 1000 CCTAAGG CC^T[ACGT]AGG
Cfr10I 183 ACCGGT [GA]^CCGG[CT]
ClaI 780 ATCGAT AT^CGAT
DraII 611 GGGACCC [GA]G^G[ACGT]CC[CT]
Eco81I 1000 CCTAAGG CC^T[ACGT]AGG
EcoNI 256 CCTCATGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 611 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRV 893 GATATC GAT^ATC
FauI 216 CCCGC CCCGC(4/6)
FspI 33 TGCGCA TGC^GCA
HgaI 1150 GACGC GACGC(5/10)
Hin1I 1150 GACGCC G[GA]^CG[CT]C
HindIII 1169 AAGCTT A^AGCTT
KspI 100 CCGCGG CCGC^GG
MflI 250 GGATCC [GA]^GATC[CT]
MroI 573 TCCGGA T^CCGGA
MseI 1374 TTAA T^TAA
MspA1I 100 CCGCGG C[AC]G^C[GT]G
NspI 593 GCATGC [GA]CATG^[CT]
PflMI 415 CCAGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 183 ACCGGT A^CCGGT
PpuMI 611 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 1150 GACGCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 149 CTGCAG CTGCA^G
SacII 100 CCGCGG CCGC^GG
SapI 1129 GCTCTTC GCTCTTC(1/4)
SfcI 149 CTGCAG C^T[GA][CT]AG
SmlI 888 CTCAAG C^T[CT][GA]AG
SphI 593 GCATGC GCATG^C
TaqI 781 TCGA T^CGA
Tru9I 1374 TTAA T^TAA
Tsp509I 1014 AATT ^AATT
TthHB8I 781 TCGA T^CGA
Van91I 415 CCAGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 250 GGATCC [GA]^GATC[CT]
XmnI 1300 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
BanI 180 GGCACC G^G[CT][GA]CC
212 GGCACC
BglI 533 GCCTCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
990 GCCTGCTGGGC
BlpI 913 GCTGAGC GC^T[ACGT]AGC
957 GCTCAGC
BpmI 413 CTCCAG CTGGAG(16/14)
355 CTGGAG
Bpu1102I 913 GCTGAGC GC^T[ACGT]AGC
957 GCTCAGC
BsaWI 183 ACCGGT [AT]^CCGG[AT]
573 TCCGGA
BseRI 1331 CTCCTC GAGGAG(10/8)
1433 GAGGAG
BsgI 452 CTGCAC GTGCAG(16/14)
440 GTGCAG
BspHI 258 TCATGA T^CATGA
1296 TCATGA
BsrDI 381 CATTGC GCAATG(2/0)
787 CATTGC
CelII 913 GCTGAGC GC^T[ACGT]AGC
957 GCTCAGC
EcoT22I 595 ATGCAT ATGCA^T
830 ATGCAT
MaeII 306 ACGT A^CGT
456 ACGT
NheI 16 GCTAGC G^CTAGC
524 GCTAGC
NsiI 595 ATGCAT ATGCA^T
830 ATGCAT
RcaI 258 TCATGA T^CATGA
1296 TCATGA
TspRI 287 CACTG CA[GC]TG[ACGT][ACGT]^
638 CACTG
XcmI 132 CCAACTTTGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
855 CCATATTCGTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaII BalI BanII BbvI
BcnI BfaI BmyI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
BstXI Cac8I CfoI Cfr13I DdeI
DpnII DsaI EaeI EarI EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MluNI MnlI MscI MslI MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI TseI
Tsp45I