##################################
# NO CUT ENZYMES ( 108 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AflII AflIII AgeI AhdI AocI
Aor51HI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII AvrII
BcgI BciVI BclI BfrI BlnI
BsaWI BseAI BsiWI BsmBI BsmI
BsoBI BspDI BspEI BspLU11I BsrDI
BssHII Bst1107I BstAPI BstEII BstPI
BstUI BstZ17I Bsu36I ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV FbaI FseI FspI HgaI
HindIII HinfI HpaI KpnI KspI
MfeI MluI MroI MseI MunI
MvnI NaeI NcoI NdeI NgoMIV
NheI NotI NruI PacI PaeR7I
PflFI PinAI PleI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SgrAI SmaI
SnaBI SpeI Sse8387I SspI StuI
SwaI TfiI Tru9I Tth111I XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 947 GACGTC GACGT^C
AcsI 1315 AAATTT [GA]^AATT[CT]
ApaI 1470 GGGCCC GGGCC^C
ApoI 1315 AAATTT [GA]^AATT[CT]
Asp700I 646 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 1180 GGATCC G^GATCC
BbeI 1209 GGCGCC GGCGC^C
BbsI 1672 GAAGAC GAAGAC(2/6)
BfaI 510 CTAG C^TAG
BglI 1463 GCCTCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 619 AGATCT A^GATCT
BpuAI 1672 GAAGAC GAAGAC(2/6)
BsaBI 487 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1661 GAGACC GGTCTC(1/5)
BsgI 190 CTGCAC GTGCAG(16/14)
BsiEI 83 CGACCG CG[GA][CT]^CG
Bsp1407I 312 TGTACA T^GTACA
BspHI 483 TCATGA T^CATGA
BspMI 1443 ACCTGC ACCTGC(4/8)
BsrFI 180 GCCGGT [GA]^CCGG[CT]
BsrGI 312 TGTACA T^GTACA
BssSI 129 CACGAG CACGAG(-5/-1)
BstBI 1302 TTCGAA TT^CGAA
BstXI 1198 CCACAGTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 180 GCCGGT [GA]^CCGG[CT]
DraIII 1919 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 25 CCTGGACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 1209 GGCGCC [GA]GCGC^[CT]
HincII 1850 GTTGAC GT[CT]^[GA]AC
HindII 1850 GTTGAC GT[CT]^[GA]AC
KasI 1209 GGCGCC G^GCGCC
MaeI 510 CTAG C^TAG
MamI 487 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 1209 GGCGCC GG^CGCC
NspV 1302 TTCGAA TT^CGAA
PpuMI 1233 AGGACCC [GA]G^G[AT]CC[CT]
RcaI 483 TCATGA T^CATGA
SexAI 30 ACCAGGT A^CC[AT]GGT
SfiI 1462 GGCCTCCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1209 GGCGCC GGC^GCC
SfuI 1302 TTCGAA TT^CGAA
SspBI 312 TGTACA T^GTACA
Tsp509I 1316 AATT ^AATT
XcmI 396 CCAACCTGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 646 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AcyI 947 GACGTC G[GA]^CG[CT]C
1209 GGCGCC
Alw44I 762 GTGCAC G^TGCAC
1652 GTGCAC
AlwNI 524 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
543 CAGCCGCTG
ApaLI 762 GTGCAC G^TGCAC
1652 GTGCAC
BalI 1522 TGGCCA TGG^CCA
1894 TGGCCA
BbrPI 759 CACGTG CAC^GTG
1666 CACGTG
BbvCI 709 GCTGAGG CCTCAGC(-5/-2)
1681 GCTGAGG
BlpI 88 GCTGAGC GC^T[ACGT]AGC
770 GCTCAGC
Bpu1102I 88 GCTGAGC GC^T[ACGT]AGC
770 GCTCAGC
BsaAI 759 CACGTG [CT]AC^GT[GA]
1666 CACGTG
BsaHI 947 GACGTC G[GA]^CG[CT]C
1209 GGCGCC
BsmAI 155 GAGAC GTCTC(1/5)
1661 GAGAC
BsrBI 1555 GAGCGG CCGCTC(-3/-3)
1868 GAGCGG
CelII 88 GCTGAGC GC^T[ACGT]AGC
770 GCTCAGC
CfoI 1210 GCGC GCG^C
1447 GCGC
DsaI 1545 CCGTGG C^C[GA][CT]GG
1734 CCGTGG
EaeI 1522 TGGCCA [CT]^GGCC[GA]
1894 TGGCCA
EarI 457 GAAGAG CTCTTC(1/4)
646 GAAGAG
EcoT22I 73 ATGCAT ATGCA^T
99 ATGCAT
FauI 199 CCCGC CCCGC(4/6)
1870 GCGGG
HhaI 1210 GCGC GCG^C
1447 GCGC
Hin1I 947 GACGTC G[GA]^CG[CT]C
1209 GGCGCC
HinP1I 1210 GCGC G^CGC
1447 GCGC
Ksp632I 457 GAAGAG CTCTTC(1/4)
646 GAAGAG
MaeIII 21 GTGAC ^GT[ACGT]AC
1265 GTCAC
MluNI 1522 TGGCCA TGG^CCA
1894 TGGCCA
MscI 1522 TGGCCA TGG^CCA
1894 TGGCCA
NsiI 73 ATGCAT ATGCA^T
99 ATGCAT
NspI 101 GCATGC [GA]CATG^[CT]
337 GCATGC
PmaCI 759 CACGTG CAC^GTG
1666 CACGTG
PmlI 759 CACGTG CAC^GTG
1666 CACGTG
SacI 163 GAGCTC GAGCT^C
266 GAGCTC
SapI 457 GAAGAGC GCTCTTC(1/4)
646 GAAGAGC
SmlI 272 CTCAAG C^T[CT][GA]AG
571 CTCAAG
SphI 101 GCATGC GCATG^C
337 GCATGC
Tsp45I 21 GTGAC ^GT[GC]AC
1265 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BanI BanII BbvI BcnI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HpaII HphI ItaI MaeII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PflMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TseI
TspRI TthHB8I Van91I XhoII