################################## # NO CUT ENZYMES ( 114 enzymes) # ################################## AatII AccI AccII AccIII AclI AflII AflIII AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I AspEI AviII BamHI BbrPI BciVI BclI BfrI BglI BlpI Bpu1102I BsaAI BsaBI BsaI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I CelII ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KspI MamI MfeI MluI MroI MunI MvnI NcoI NdeI NotI NruI NsiI NspV PacI PaeR7I PflMI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SbfI SfiI SfuI SgrAI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI TaqI TthHB8I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### Acc65I 318 GGTACC G^GTACC AgeI 170 ACCGGT A^CCGGT Asp718I 318 GGTACC G^GTACC AspHI 365 GTGCTC G[AT]GC[AT]^C AspI 983 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 31 CCTAGG C^CTAGG BalI 508 TGGCCA TGG^CCA BbeI 1222 GGCGCC GGCGC^C BbvCI 559 CCTCAGC CCTCAGC(-5/-2) BcgI 548 CGACGCCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 1354 AGATCT A^GATCT BlnI 31 CCTAGG C^CTAGG BsaWI 170 ACCGGT [AT]^CCGG[AT] BsiHKAI 365 GTGCTC G[AT]GC[AT]^C BstAPI 695 GCAGCCGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 1191 CCAGCTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DrdI 1054 GACAGACGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 508 TGGCCA [CT]^GGCC[GA] EcoRI 455 GAATTC G^AATTC FauI 352 CCCGC CCCGC(4/6) KasI 1222 GGCGCC G^GCGCC KpnI 318 GGTACC GGTAC^C MluNI 508 TGGCCA TGG^CCA MscI 508 TGGCCA TGG^CCA NaeI 1508 GCCGGC GCC^GGC NarI 1222 GGCGCC GG^CGCC NgoMIV 1508 GCCGGC G^CCGGC NspI 423 GCATGC [GA]CATG^[CT] PflFI 983 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 170 ACCGGT A^CCGGT PpuMI 296 AGGTCCC [GA]G^G[AT]CC[CT] PshAI 1540 GACGGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SapI 1292 GAAGAGC GCTCTTC(1/4) ScaI 938 AGTACT AGT^ACT SexAI 447 ACCAGGT A^CC[AT]GGT SfcI 914 CTACAG C^T[GA][CT]AG SfoI 1222 GGCGCC GGC^GCC SphI 423 GCATGC GCATG^C Tth111I 983 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 1219 CCAGGCGCCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AciI 285 CCGC CCGC(-3/-1) 353 CCGC AcsI 455 GAATTC [GA]^AATT[CT] 1265 GAATTT AlwI 385 GATCC GGATC(4/5) 844 GATCC AlwNI 1138 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG 1388 CAGACACTG ApoI 455 GAATTC [GA]^AATT[CT] 1265 GAATTT AvaI 765 CCCGGG C^[CT]CG[GA]G 1032 CCCGGG BsmAI 933 GAGAC GTCTC(1/5) 1052 GAGAC BsoBI 765 CCCGGG C^[CT]CG[GA]G 1032 CCCGGG BsrDI 665 GCAATG GCAATG(2/0) 1503 GCAATG BsrFI 170 ACCGGT [GA]^CCGG[CT] 1508 GCCGGC BstYI 384 AGATCC [GA]^GATC[CT] 1354 AGATCT Cfr10I 170 ACCGGT [GA]^CCGG[CT] 1508 GCCGGC DsaI 302 CCACGG C^C[GA][CT]GG 1287 CCGTGG EcoT14I 31 CCTAGG C^C[AT][AT]GG 1085 CCTTGG HincII 395 GTCAAC GT[CT]^[GA]AC 1409 GTCAAC HindII 395 GTCAAC GT[CT]^[GA]AC 1409 GTCAAC MaeII 63 ACGT A^CGT 339 ACGT MflI 384 AGATCC [GA]^GATC[CT] 1354 AGATCT MseI 584 TTAA T^TAA 594 TTAA MslI 358 CACCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 370 CACCCAGATG NheI 346 GCTAGC G^CTAGC 542 GCTAGC SfaNI 1558 GATGC GCATC(5/9) 624 GCATC SmaI 765 CCCGGG CCC^GGG 1032 CCCGGG StyI 31 CCTAGG C^C[AT][AT]GG 1085 CCTTGG Tru9I 584 TTAA T^TAA 594 TTAA XhoII 384 AGATCC [GA]^GATC[CT] 1354 AGATCT XmaCI 765 CCCGGG C^CCGGG 1032 CCCGGG XmaI 765 CCCGGG C^CCGGG 1032 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 76 enzymes) # ######################################### AcyI AfaI AluI AocI AvaII BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsgI BsiYI BslI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII EarI Eco81I EcoO109I EcoRII Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PvuII RsaI Sau3AI Sau96I ScrFI TfiI TseI Tsp45I Tsp509I TspRI