##################################
# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AflII AflIII AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I AspEI AviII BamHI BbrPI
BciVI BclI BfrI BglI BlpI
Bpu1102I BsaAI BsaBI BsaI BseAI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I CelII
ClaI CpoI DraI DraIII EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HindIII HpaI KspI MamI
MfeI MluI MroI MunI MvnI
NcoI NdeI NotI NruI NsiI
NspV PacI PaeR7I PflMI PmaCI
PmeI PmlI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SbfI SfiI SfuI SgrAI
SmlI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI TaqI TthHB8I
Van91I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 318 GGTACC G^GTACC
AgeI 170 ACCGGT A^CCGGT
Asp718I 318 GGTACC G^GTACC
AspHI 365 GTGCTC G[AT]GC[AT]^C
AspI 983 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 31 CCTAGG C^CTAGG
BalI 508 TGGCCA TGG^CCA
BbeI 1222 GGCGCC GGCGC^C
BbvCI 559 CCTCAGC CCTCAGC(-5/-2)
BcgI 548 CGACGCCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1354 AGATCT A^GATCT
BlnI 31 CCTAGG C^CTAGG
BsaWI 170 ACCGGT [AT]^CCGG[AT]
BsiHKAI 365 GTGCTC G[AT]GC[AT]^C
BstAPI 695 GCAGCCGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1191 CCAGCTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 1054 GACAGACGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 508 TGGCCA [CT]^GGCC[GA]
EcoRI 455 GAATTC G^AATTC
FauI 352 CCCGC CCCGC(4/6)
KasI 1222 GGCGCC G^GCGCC
KpnI 318 GGTACC GGTAC^C
MluNI 508 TGGCCA TGG^CCA
MscI 508 TGGCCA TGG^CCA
NaeI 1508 GCCGGC GCC^GGC
NarI 1222 GGCGCC GG^CGCC
NgoMIV 1508 GCCGGC G^CCGGC
NspI 423 GCATGC [GA]CATG^[CT]
PflFI 983 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 170 ACCGGT A^CCGGT
PpuMI 296 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 1540 GACGGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 1292 GAAGAGC GCTCTTC(1/4)
ScaI 938 AGTACT AGT^ACT
SexAI 447 ACCAGGT A^CC[AT]GGT
SfcI 914 CTACAG C^T[GA][CT]AG
SfoI 1222 GGCGCC GGC^GCC
SphI 423 GCATGC GCATG^C
Tth111I 983 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1219 CCAGGCGCCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AciI 285 CCGC CCGC(-3/-1)
353 CCGC
AcsI 455 GAATTC [GA]^AATT[CT]
1265 GAATTT
AlwI 385 GATCC GGATC(4/5)
844 GATCC
AlwNI 1138 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1388 CAGACACTG
ApoI 455 GAATTC [GA]^AATT[CT]
1265 GAATTT
AvaI 765 CCCGGG C^[CT]CG[GA]G
1032 CCCGGG
BsmAI 933 GAGAC GTCTC(1/5)
1052 GAGAC
BsoBI 765 CCCGGG C^[CT]CG[GA]G
1032 CCCGGG
BsrDI 665 GCAATG GCAATG(2/0)
1503 GCAATG
BsrFI 170 ACCGGT [GA]^CCGG[CT]
1508 GCCGGC
BstYI 384 AGATCC [GA]^GATC[CT]
1354 AGATCT
Cfr10I 170 ACCGGT [GA]^CCGG[CT]
1508 GCCGGC
DsaI 302 CCACGG C^C[GA][CT]GG
1287 CCGTGG
EcoT14I 31 CCTAGG C^C[AT][AT]GG
1085 CCTTGG
HincII 395 GTCAAC GT[CT]^[GA]AC
1409 GTCAAC
HindII 395 GTCAAC GT[CT]^[GA]AC
1409 GTCAAC
MaeII 63 ACGT A^CGT
339 ACGT
MflI 384 AGATCC [GA]^GATC[CT]
1354 AGATCT
MseI 584 TTAA T^TAA
594 TTAA
MslI 358 CACCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
370 CACCCAGATG
NheI 346 GCTAGC G^CTAGC
542 GCTAGC
SfaNI 1558 GATGC GCATC(5/9)
624 GCATC
SmaI 765 CCCGGG CCC^GGG
1032 CCCGGG
StyI 31 CCTAGG C^C[AT][AT]GG
1085 CCTTGG
Tru9I 584 TTAA T^TAA
594 TTAA
XhoII 384 AGATCC [GA]^GATC[CT]
1354 AGATCT
XmaCI 765 CCCGGG C^CCGGG
1032 CCCGGG
XmaI 765 CCCGGG C^CCGGG
1032 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AcyI AfaI AluI AocI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaHI
BsaJI BseRI BsgI BsiYI BslI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII EarI
Eco81I EcoO109I EcoRII Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MnlI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PvuII RsaI Sau3AI Sau96I
ScrFI TfiI TseI Tsp45I Tsp509I
TspRI