################################## # NO CUT ENZYMES ( 126 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AgeI AhdI Alw44I AocI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BbeI BcgI BclI BcnI BfrI BglI BlnI BsaBI BsaHI BsaWI BseAI BsiHKAI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DraIII Eam1105I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FspI Hin1I HindIII HpaI KasI KpnI MamI MfeI MluI MluNI MroI MscI MseI MunI NarI NciI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SmaI SnaBI SpeI SphI Sse8387I SspBI SwaI TfiI Tru9I Tsp509I Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccII 87 CGCG CG^CG AlwNI 965 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 881 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BbrPI 377 CACGTG CAC^GTG BbsI 392 GTCTTC GAAGAC(2/6) BbvCI 983 GCTGAGG CCTCAGC(-5/-2) BciVI 664 GGATAC GTATCC(6/5) BfaI 1287 CTAG C^TAG BglII 672 AGATCT A^GATCT BpuAI 392 GTCTTC GAAGAC(2/6) BsaI 335 GAGACC GGTCTC(1/5) BsgI 1100 GTGCAG GTGCAG(16/14) BsiEI 99 CGGCCG CG[GA][CT]^CG BsiWI 160 CGTACG C^GTACG BsmBI 1160 GAGACG CGTCTC(1/5) BstUI 87 CGCG CG^CG DrdI 187 GACCGAAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 99 CGGCCG [CT]^GGCC[GA] EagI 99 CGGCCG C^GGCCG EarI 710 GAAGAG CTCTTC(1/4) EclXI 99 CGGCCG C^GGCCG Eco52I 99 CGGCCG C^GGCCG EcoT14I 432 CCATGG C^C[AT][AT]GG FseI 96 GGCCGGCC GGCCGG^CC HgaI 980 GACGC GACGC(5/10) HincII 184 GTTGAC GT[CT]^[GA]AC HindII 184 GTTGAC GT[CT]^[GA]AC Ksp632I 710 GAAGAG CTCTTC(1/4) KspI 86 CCGCGG CCGC^GG MaeI 1287 CTAG C^TAG MaeIII 1206 GTGAC ^GT[ACGT]AC MslI 770 CACATGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 87 CGCG CG^CG NcoI 432 CCATGG C^CATGG NdeI 50 CATATG CA^TATG PmaCI 377 CACGTG CAC^GTG PmlI 377 CACGTG CAC^GTG PvuII 1122 CAGCTG CAG^CTG SacII 86 CCGCGG CCGC^GG SspI 744 AATATT AAT^ATT StuI 312 AGGCCT AGG^CCT StyI 432 CCATGG C^C[AT][AT]GG Tsp45I 1206 GTGAC ^GT[GC]AC XmnI 881 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AflIII 157 ACACGT A^C[GA][CT]GT 378 ACGTGT AlwI 118 GATCC GGATC(4/5) 760 GATCC BanI 1016 GGCACC G^G[CT][GA]CC 1136 GGCACC BlpI 608 GCTCAGC GC^T[ACGT]AGC 1124 GCTGAGC Bpu1102I 608 GCTCAGC GC^T[ACGT]AGC 1124 GCTGAGC BstF5I 640 GGATG GGATG(2/0) 1156 GGATG BstYI 672 AGATCT [GA]^GATC[CT] 759 AGATCC CelII 608 GCTCAGC GC^T[ACGT]AGC 1124 GCTGAGC FokI 640 GGATG GGATG(9/13) 1156 GGATG MflI 672 AGATCT [GA]^GATC[CT] 759 AGATCC NaeI 97 GCCGGC GCC^GGC 463 GCCGGC NgoMIV 97 GCCGGC G^CCGGC 463 GCCGGC NlaIII 433 CATG CATG^ 772 CATG PflMI 786 CCAAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1266 CCAGCCTGTGG PstI 122 CTGCAG CTGCA^G 1022 CTGCAG SgrAI 58 CACCGGCG C[GA]^CCGG[CT]G 277 CACCGGCG SmlI 1034 CTCAAG C^T[CT][GA]AG 1293 CTTGAG TaqI 678 TCGA T^CGA 930 TCGA TthHB8I 678 TCGA T^CGA 930 TCGA Van91I 786 CCAAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1266 CCAGCCTGTGG XhoII 672 AGATCT [GA]^GATC[CT] 759 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 67 enzymes) # ######################################### AciI AfaI AluI Aor51HI AvaII BanII BbvI BmyI BpmI BsaAI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrFI BsrI BssKI BstNI BstXI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI Eco47III EcoNI EcoO109I EcoRII FauI Fnu4HI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MboI MboII MnlI MspA1I MspI MvaI MwoI NdeII NlaIV PleI PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TseI TspRI XcmI