##################################
# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I AocI ApaI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BbeI BcgI BclI
BcnI BfrI BglI BlnI BsaBI
BsaHI BsaWI BseAI BsiHKAI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DraI DraIII Eam1105I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FspI
Hin1I HindIII HpaI KasI KpnI
MamI MfeI MluI MluNI MroI
MscI MseI MunI NarI NciI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SmaI SnaBI SpeI SphI
Sse8387I SspBI SwaI TfiI Tru9I
Tsp509I Tth111I XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccII 87 CGCG CG^CG
AlwNI 965 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 881 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbrPI 377 CACGTG CAC^GTG
BbsI 392 GTCTTC GAAGAC(2/6)
BbvCI 983 GCTGAGG CCTCAGC(-5/-2)
BciVI 664 GGATAC GTATCC(6/5)
BfaI 1287 CTAG C^TAG
BglII 672 AGATCT A^GATCT
BpuAI 392 GTCTTC GAAGAC(2/6)
BsaI 335 GAGACC GGTCTC(1/5)
BsgI 1100 GTGCAG GTGCAG(16/14)
BsiEI 99 CGGCCG CG[GA][CT]^CG
BsiWI 160 CGTACG C^GTACG
BsmBI 1160 GAGACG CGTCTC(1/5)
BstUI 87 CGCG CG^CG
DrdI 187 GACCGAAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 99 CGGCCG [CT]^GGCC[GA]
EagI 99 CGGCCG C^GGCCG
EarI 710 GAAGAG CTCTTC(1/4)
EclXI 99 CGGCCG C^GGCCG
Eco52I 99 CGGCCG C^GGCCG
EcoT14I 432 CCATGG C^C[AT][AT]GG
FseI 96 GGCCGGCC GGCCGG^CC
HgaI 980 GACGC GACGC(5/10)
HincII 184 GTTGAC GT[CT]^[GA]AC
HindII 184 GTTGAC GT[CT]^[GA]AC
Ksp632I 710 GAAGAG CTCTTC(1/4)
KspI 86 CCGCGG CCGC^GG
MaeI 1287 CTAG C^TAG
MaeIII 1206 GTGAC ^GT[ACGT]AC
MslI 770 CACATGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 87 CGCG CG^CG
NcoI 432 CCATGG C^CATGG
NdeI 50 CATATG CA^TATG
PmaCI 377 CACGTG CAC^GTG
PmlI 377 CACGTG CAC^GTG
PvuII 1122 CAGCTG CAG^CTG
SacII 86 CCGCGG CCGC^GG
SspI 744 AATATT AAT^ATT
StuI 312 AGGCCT AGG^CCT
StyI 432 CCATGG C^C[AT][AT]GG
Tsp45I 1206 GTGAC ^GT[GC]AC
XmnI 881 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AflIII 157 ACACGT A^C[GA][CT]GT
378 ACGTGT
AlwI 118 GATCC GGATC(4/5)
760 GATCC
BanI 1016 GGCACC G^G[CT][GA]CC
1136 GGCACC
BlpI 608 GCTCAGC GC^T[ACGT]AGC
1124 GCTGAGC
Bpu1102I 608 GCTCAGC GC^T[ACGT]AGC
1124 GCTGAGC
BstF5I 640 GGATG GGATG(2/0)
1156 GGATG
BstYI 672 AGATCT [GA]^GATC[CT]
759 AGATCC
CelII 608 GCTCAGC GC^T[ACGT]AGC
1124 GCTGAGC
FokI 640 GGATG GGATG(9/13)
1156 GGATG
MflI 672 AGATCT [GA]^GATC[CT]
759 AGATCC
NaeI 97 GCCGGC GCC^GGC
463 GCCGGC
NgoMIV 97 GCCGGC G^CCGGC
463 GCCGGC
NlaIII 433 CATG CATG^
772 CATG
PflMI 786 CCAAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1266 CCAGCCTGTGG
PstI 122 CTGCAG CTGCA^G
1022 CTGCAG
SgrAI 58 CACCGGCG C[GA]^CCGG[CT]G
277 CACCGGCG
SmlI 1034 CTCAAG C^T[CT][GA]AG
1293 CTTGAG
TaqI 678 TCGA T^CGA
930 TCGA
TthHB8I 678 TCGA T^CGA
930 TCGA
Van91I 786 CCAAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1266 CCAGCCTGTGG
XhoII 672 AGATCT [GA]^GATC[CT]
759 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AfaI AluI Aor51HI AvaII
BanII BbvI BmyI BpmI BsaAI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrFI BsrI
BssKI BstNI BstXI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI Eco47III EcoNI EcoO109I EcoRII
FauI Fnu4HI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MboI MboII MnlI
MspA1I MspI MvaI MwoI NdeII
NlaIV PleI PpuMI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TseI
TspRI XcmI