##################################
# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BbeI BbsI BcgI BciVI BclI
BfaI BfrI BglI BglII BlnI
BpuAI BsaBI BsaHI BsaWI BseAI
BseRI BsiEI BsiWI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspEI BspHI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I Cfr10I
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI Hin1I HpaI KasI
KpnI KspI MaeI MamI MfeI
MluI MroI MseI MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PpuMI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tru9I Tsp509I Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 514 GTCTAC GT^[AC][GT]AC
ApaI 298 GGGCCC GGGCC^C
Asp700I 75 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 259 GAGCAC G[AT]GC[AT]^C
BalI 17 TGGCCA TGG^CCA
BanI 247 GGCACC G^G[CT][GA]CC
BanII 298 GGGCCC G[GA]GC[CT]^C
BbrPI 431 CACGTG CAC^GTG
BbvCI 540 CCTCAGC CCTCAGC(-5/-2)
BcnI 71 CCCGG CC^[GC]GG
BlpI 256 GCTGAGC GC^T[ACGT]AGC
BpmI 631 CTGGAG CTGGAG(16/14)
Bpu1102I 256 GCTGAGC GC^T[ACGT]AGC
BsaAI 431 CACGTG [CT]AC^GT[GA]
BsaI 400 GGTCTC GGTCTC(1/5)
BsiHKAI 259 GAGCAC G[AT]GC[AT]^C
BsmAI 401 GTCTC GTCTC(1/5)
BspDI 187 ATCGAT AT^CGAT
BspLU11I 561 ACATGT A^CATGT
BsrDI 377 CATTGC GCAATG(2/0)
CelII 256 GCTGAGC GC^T[ACGT]AGC
ClaI 187 ATCGAT AT^CGAT
DraII 298 GGGCCCC [GA]G^G[ACGT]CC[CT]
EaeI 17 TGGCCA [CT]^GGCC[GA]
EarI 493 CTCTTC CTCTTC(1/4)
EcoO109I 298 GGGCCCC [GA]G^G[ACGT]CC[CT]
HgaI 330 GCGTC GACGC(5/10)
HincII 565 GTCAAC GT[CT]^[GA]AC
HindII 565 GTCAAC GT[CT]^[GA]AC
HindIII 125 AAGCTT A^AGCTT
HphI 162 GGTGA GGTGA(8/7)
Ksp632I 493 CTCTTC CTCTTC(1/4)
MaeII 432 ACGT A^CGT
MaeIII 665 GTCAC ^GT[ACGT]AC
MluNI 17 TGGCCA TGG^CCA
MscI 17 TGGCCA TGG^CCA
MslI 262 CACTGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 71 CCCGG CC^[GC]GG
PmaCI 431 CACGTG CAC^GTG
PmlI 431 CACGTG CAC^GTG
PshAI 325 GACGAGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 264 CTGCAG CTGCA^G
SfcI 264 CTGCAG C^T[GA][CT]AG
SmlI 133 CTCAAG C^T[CT][GA]AG
Tsp45I 665 GTCAC ^GT[GC]AC
XmnI 75 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AfaI 312 GTAC GT^AC
336 GTAC
Aor51HI 77 AGCGCT AGC^GCT
98 AGCGCT
AvaII 147 GGACC G^G[AT]CC
618 GGACC
BmyI 259 GAGCAC G[AGT]GC[ACT]^C
298 GGGCCC
BsgI 6 CTGCAC GTGCAG(16/14)
471 CTGCAC
Bsp1286I 259 GAGCAC G[AGT]GC[ACT]^C
298 GGGCCC
BspMI 571 ACCTGC ACCTGC(4/8)
465 GCAGGT
BstYI 479 AGATCC [GA]^GATC[CT]
659 GGATCT
Cac8I 317 GCTGGC GC[ACGT]^[ACGT]GC
345 GCCCGC
CfoI 78 GCGC GCG^C
99 GCGC
DsaI 20 CCATGG C^C[GA][CT]GG
251 CCATGG
Eco47III 77 AGCGCT AGC^GCT
98 AGCGCT
EcoT14I 20 CCATGG C^C[AT][AT]GG
251 CCATGG
HaeII 77 AGCGCT [GA]GCGC^[CT]
98 AGCGCT
HhaI 78 GCGC GCG^C
99 GCGC
HinP1I 78 GCGC G^CGC
99 GCGC
MboII 221 TCTTC GAAGA(8/7)
494 TCTTC
MflI 479 AGATCC [GA]^GATC[CT]
659 GGATCT
NcoI 20 CCATGG C^CATGG
251 CCATGG
NspI 207 GCATGT [GA]CATG^[CT]
561 ACATGT
PvuII 3 CAGCTG CAG^CTG
276 CAGCTG
RsaI 312 GTAC GT^AC
336 GTAC
SfaNI 185 GCATC GCATC(5/9)
536 GCATC
StyI 20 CCATGG C^C[AT][AT]GG
251 CCATGG
XhoII 479 AGATCC [GA]^GATC[CT]
659 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AflIII AluI AlwI BbvI
BsaJI BsiYI BslI BsrI BssKI
BstF5I BstNI Cfr13I DdeI DpnII
EcoRII FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII ItaI MboI
MnlI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV PleI Sau3AI
Sau96I ScrFI TaqI TseI TspRI
TthHB8I