##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII AccI AclI AflII AgeI
AocI Aor51HI AscI AseI AsnI
Asp700I AspI AvrII BamHI BbeI
BbrPI BbvCI BfrI BglII BlnI
BsiEI BsiWI Bsp1407I BspDI BspHI
BspLU11I BsrDI BsrGI BssHII Bst1107I
BstBI BstZ17I Bsu36I ClaI CpoI
DraI DrdI EagI EclXI Eco47III
Eco52I Eco81I EcoRI FseI HincII
HindII HindIII HpaI KasI KspI
MfeI MluI MunI NaeI NarI
NdeI NgoMIV NotI NruI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI TfiI
Tth111I XbaI XcmI XmnI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
Acc65I 1690 GGTACC G^GTACC
AccII 448 CGCG CG^CG
AccIII 70 TCCGGA T^CCGGA
AcsI 1607 GAATTT [GA]^AATT[CT]
AhdI 1446 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1764 GTGCAC G^TGCAC
ApaI 1487 GGGCCC GGGCC^C
ApaLI 1764 GTGCAC G^TGCAC
ApoI 1607 GAATTT [GA]^AATT[CT]
Asp718I 1690 GGTACC G^GTACC
AspEI 1446 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1090 TGCGCA TGC^GCA
BcgI 443 CGACCCGCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 430 TGATCA T^GATCA
BlpI 359 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 359 GCTCAGC GC^T[ACGT]AGC
BsaAI 138 TACGTG [CT]AC^GT[GA]
BsaBI 116 GATCGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 70 TCCGGA T^CCGGA
BsmI 329 GAATGC GAATGC(1/-1)
BspEI 70 TCCGGA T^CCGGA
BspMI 1524 GCAGGT ACCTGC(4/8)
BssSI 544 CACGAG CACGAG(-5/-1)
BstAPI 291 GCAGTCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 799 GGTTACC G^GT[ACGT]ACC
BstPI 799 GGTTACC G^GT[ACGT]ACC
BstUI 448 CGCG CG^CG
BstYI 1601 GGATCT [GA]^GATC[CT]
CelII 359 GCTCAGC GC^T[ACGT]AGC
DraIII 1717 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1446 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 783 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 799 GGTTACC G^GT[ACGT]ACC
EcoRV 1752 GATATC GAT^ATC
EcoT22I 622 ATGCAT ATGCA^T
FbaI 430 TGATCA T^GATCA
FspI 1090 TGCGCA TGC^GCA
HaeII 340 AGCGCC [GA]GCGC^[CT]
KpnI 1690 GGTACC GGTAC^C
MamI 116 GATCGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1601 GGATCT [GA]^GATC[CT]
MroI 70 TCCGGA T^CCGGA
MseI 60 TTAA T^TAA
MvnI 448 CGCG CG^CG
NheI 1875 GCTAGC G^CTAGC
NsiI 622 ATGCAT ATGCA^T
PaeR7I 684 CTCGAG C^TCGAG
PshAI 764 GACGCCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 144 GAGCTC GAGCT^C
SmaI 750 CCCGGG CCC^GGG
SmlI 684 CTCGAG C^T[CT][GA]AG
SphI 100 GCATGC GCATG^C
Tru9I 60 TTAA T^TAA
XhoI 684 CTCGAG C^TCGAG
XhoII 1601 GGATCT [GA]^GATC[CT]
XmaCI 750 CCCGGG C^CCGGG
XmaI 750 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AflIII 311 ACGTGT A^C[GA][CT]GT
604 ACGTGT
BanII 144 GAGCTC G[GA]GC[CT]^C
1487 GGGCCC
BbsI 1779 GAAGAC GAAGAC(2/6)
1758 GTCTTC
BciVI 1016 GGATAC GTATCC(6/5)
1463 GGATAC
BfaI 105 CTAG C^TAG
1876 CTAG
BpuAI 1779 GAAGAC GAAGAC(2/6)
1758 GTCTTC
BsaWI 70 TCCGGA [AT]^CCGG[AT]
422 TCCGGT
BseRI 509 CTCCTC GAGGAG(10/8)
787 GAGGAG
BstXI 838 CCAAGGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1561 CCATTGGGTTGG
EarI 512 CTCTTC CTCTTC(1/4)
1827 CTCTTC
HinfI 153 GACTC G^A[ACGT]TC
507 GACTC
Ksp632I 512 CTCTTC CTCTTC(1/4)
1827 CTCTTC
MaeI 105 CTAG C^TAG
1876 CTAG
PflMI 1052 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1155 CCACAGCATGG
PleI 153 GACTC GAGTC(4/5)
507 GACTC
PstI 553 CTGCAG CTGCA^G
1803 CTGCAG
PvuII 591 CAGCTG CAG^CTG
971 CAGCTG
Van91I 1052 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1155 CCACAGCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BalI
BanI BbvI BcnI BglI BmyI
BpmI BsaHI BsaI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BsrBI BsrFI
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HpaII HphI ItaI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TseI
Tsp45I Tsp509I TspRI TthHB8I