################################## # NO CUT ENZYMES ( 94 enzymes) # ################################## AatII AccI AclI AflII AgeI AocI Aor51HI AscI AseI AsnI Asp700I AspI AvrII BamHI BbeI BbrPI BbvCI BfrI BglII BlnI BsiEI BsiWI Bsp1407I BspDI BspHI BspLU11I BsrDI BsrGI BssHII Bst1107I BstBI BstZ17I Bsu36I ClaI CpoI DraI DrdI EagI EclXI Eco47III Eco52I Eco81I EcoRI FseI HincII HindII HindIII HpaI KasI KspI MfeI MluI MunI NaeI NarI NdeI NgoMIV NotI NruI NspV PacI PflFI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI TfiI Tth111I XbaI XcmI XmnI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### Acc65I 1690 GGTACC G^GTACC AccII 448 CGCG CG^CG AccIII 70 TCCGGA T^CCGGA AcsI 1607 GAATTT [GA]^AATT[CT] AhdI 1446 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1764 GTGCAC G^TGCAC ApaI 1487 GGGCCC GGGCC^C ApaLI 1764 GTGCAC G^TGCAC ApoI 1607 GAATTT [GA]^AATT[CT] Asp718I 1690 GGTACC G^GTACC AspEI 1446 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 1090 TGCGCA TGC^GCA BcgI 443 CGACCCGCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 430 TGATCA T^GATCA BlpI 359 GCTCAGC GC^T[ACGT]AGC Bpu1102I 359 GCTCAGC GC^T[ACGT]AGC BsaAI 138 TACGTG [CT]AC^GT[GA] BsaBI 116 GATCGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 70 TCCGGA T^CCGGA BsmI 329 GAATGC GAATGC(1/-1) BspEI 70 TCCGGA T^CCGGA BspMI 1524 GCAGGT ACCTGC(4/8) BssSI 544 CACGAG CACGAG(-5/-1) BstAPI 291 GCAGTCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 799 GGTTACC G^GT[ACGT]ACC BstPI 799 GGTTACC G^GT[ACGT]ACC BstUI 448 CGCG CG^CG BstYI 1601 GGATCT [GA]^GATC[CT] CelII 359 GCTCAGC GC^T[ACGT]AGC DraIII 1717 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 1446 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 783 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 799 GGTTACC G^GT[ACGT]ACC EcoRV 1752 GATATC GAT^ATC EcoT22I 622 ATGCAT ATGCA^T FbaI 430 TGATCA T^GATCA FspI 1090 TGCGCA TGC^GCA HaeII 340 AGCGCC [GA]GCGC^[CT] KpnI 1690 GGTACC GGTAC^C MamI 116 GATCGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 1601 GGATCT [GA]^GATC[CT] MroI 70 TCCGGA T^CCGGA MseI 60 TTAA T^TAA MvnI 448 CGCG CG^CG NheI 1875 GCTAGC G^CTAGC NsiI 622 ATGCAT ATGCA^T PaeR7I 684 CTCGAG C^TCGAG PshAI 764 GACGCCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 144 GAGCTC GAGCT^C SmaI 750 CCCGGG CCC^GGG SmlI 684 CTCGAG C^T[CT][GA]AG SphI 100 GCATGC GCATG^C Tru9I 60 TTAA T^TAA XhoI 684 CTCGAG C^TCGAG XhoII 1601 GGATCT [GA]^GATC[CT] XmaCI 750 CCCGGG C^CCGGG XmaI 750 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AflIII 311 ACGTGT A^C[GA][CT]GT 604 ACGTGT BanII 144 GAGCTC G[GA]GC[CT]^C 1487 GGGCCC BbsI 1779 GAAGAC GAAGAC(2/6) 1758 GTCTTC BciVI 1016 GGATAC GTATCC(6/5) 1463 GGATAC BfaI 105 CTAG C^TAG 1876 CTAG BpuAI 1779 GAAGAC GAAGAC(2/6) 1758 GTCTTC BsaWI 70 TCCGGA [AT]^CCGG[AT] 422 TCCGGT BseRI 509 CTCCTC GAGGAG(10/8) 787 GAGGAG BstXI 838 CCAAGGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1561 CCATTGGGTTGG EarI 512 CTCTTC CTCTTC(1/4) 1827 CTCTTC HinfI 153 GACTC G^A[ACGT]TC 507 GACTC Ksp632I 512 CTCTTC CTCTTC(1/4) 1827 CTCTTC MaeI 105 CTAG C^TAG 1876 CTAG PflMI 1052 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1155 CCACAGCATGG PleI 153 GACTC GAGTC(4/5) 507 GACTC PstI 553 CTGCAG CTGCA^G 1803 CTGCAG PvuII 591 CAGCTG CAG^CTG 971 CAGCTG Van91I 1052 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1155 CCACAGCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 89 enzymes) # ######################################### AciI AcyI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BalI BanI BbvI BcnI BglI BmyI BpmI BsaHI BsaI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BsrBI BsrFI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII HphI ItaI MaeII MaeIII MboI MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I