##################################
# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AviII AvrII BanI BbeI
BbvCI BfrI BglII BlnI BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspMI BsrFI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstUI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FseI FspI HaeII
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MslI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TaqI Tth111I TthHB8I XbaI
XcmI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 1286 GTAGAC GT^[AC][GT]AC
AcsI 1321 AAATTT [GA]^AATT[CT]
ApaI 705 GGGCCC GGGCC^C
ApoI 1321 AAATTT [GA]^AATT[CT]
BamHI 511 GGATCC G^GATCC
BbrPI 766 CACGTG CAC^GTG
BbsI 810 GAAGAC GAAGAC(2/6)
BcgI 1039 CGAATCTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 530 GTATCC GTATCC(6/5)
BclI 770 TGATCA T^GATCA
BglI 26 GCCTCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 396 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 396 GCTGAGC GC^T[ACGT]AGC
BpuAI 810 GAAGAC GAAGAC(2/6)
BsaI 72 GAGACC GGTCTC(1/5)
BseRI 856 GAGGAG GAGGAG(10/8)
BsrBI 507 GAGCGG CCGCTC(-3/-3)
BsrDI 600 CATTGC GCAATG(2/0)
BssSI 557 CTCGTG CACGAG(-5/-1)
BstAPI 967 GCAAGTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 371 CCAGCGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 396 GCTGAGC GC^T[ACGT]AGC
DsaI 866 CCACGG C^C[GA][CT]GG
EarI 1368 GAAGAG CTCTTC(1/4)
EcoT14I 1344 CCAAGG C^C[AT][AT]GG
FbaI 770 TGATCA T^GATCA
Ksp632I 1368 GAAGAG CTCTTC(1/4)
MspA1I 228 CAGCTG C[AC]G^C[GT]G
PflMI 695 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1214 GAGTC GAGTC(4/5)
PmaCI 766 CACGTG CAC^GTG
PmlI 766 CACGTG CAC^GTG
PvuII 228 CAGCTG CAG^CTG
SfiI 25 GGCCTCCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 1208 CTCAAG C^T[CT][GA]AG
StyI 1344 CCAAGG C^C[AT][AT]GG
Tsp509I 1322 AATT ^AATT
Van91I 695 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AfaI 102 GTAC GT^AC
1237 GTAC
AflIII 469 ACATGT A^C[GA][CT]GT
1246 ACATGT
AvaI 417 CCCGAG C^[CT]CG[GA]G
618 CTCGGG
BalI 683 TGGCCA TGG^CCA
989 TGGCCA
BcnI 365 CCGGG CC^[GC]GG
582 CCGGG
BfaI 115 CTAG C^TAG
998 CTAG
BpmI 1080 CTGGAG CTGGAG(16/14)
1102 CTGGAG
BsaAI 766 CACGTG [CT]AC^GT[GA]
1328 TACGTG
BsoBI 417 CCCGAG C^[CT]CG[GA]G
618 CTCGGG
BspLU11I 469 ACATGT A^CATGT
1246 ACATGT
BsrI 1358 ACTGG ACTGG(1/-1)
686 CCAGT
CfoI 329 GCGC GCG^C
374 GCGC
HhaI 329 GCGC GCG^C
374 GCGC
HinP1I 329 GCGC G^CGC
374 GCGC
MaeI 115 CTAG C^TAG
998 CTAG
MluNI 683 TGGCCA TGG^CCA
989 TGGCCA
MscI 683 TGGCCA TGG^CCA
989 TGGCCA
MseI 1167 TTAA T^TAA
1420 TTAA
NciI 365 CCGGG CC^[GC]GG
582 CCGGG
NspI 469 ACATGT [GA]CATG^[CT]
1246 ACATGT
RsaI 102 GTAC GT^AC
1237 GTAC
StuI 24 AGGCCT AGG^CCT
440 AGGCCT
Tru9I 1167 TTAA T^TAA
1420 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AluI AlwI AlwNI AvaII
BanII BbvI BmyI BsaJI BsiYI
BslI BsmAI BsmFI Bsp1286I BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MflI MnlI
MspI MvaI MwoI NdeII NlaIII
NlaIV PpuMI Sau3AI Sau96I ScrFI
SfaNI SfcI TfiI TseI Tsp45I
TspRI XhoII