################################## # NO CUT ENZYMES ( 140 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BanI BbeI BbvCI BfrI BglII BlnI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspMI BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MslI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AccI 1286 GTAGAC GT^[AC][GT]AC AcsI 1321 AAATTT [GA]^AATT[CT] ApaI 705 GGGCCC GGGCC^C ApoI 1321 AAATTT [GA]^AATT[CT] BamHI 511 GGATCC G^GATCC BbrPI 766 CACGTG CAC^GTG BbsI 810 GAAGAC GAAGAC(2/6) BcgI 1039 CGAATCTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 530 GTATCC GTATCC(6/5) BclI 770 TGATCA T^GATCA BglI 26 GCCTCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 396 GCTGAGC GC^T[ACGT]AGC Bpu1102I 396 GCTGAGC GC^T[ACGT]AGC BpuAI 810 GAAGAC GAAGAC(2/6) BsaI 72 GAGACC GGTCTC(1/5) BseRI 856 GAGGAG GAGGAG(10/8) BsrBI 507 GAGCGG CCGCTC(-3/-3) BsrDI 600 CATTGC GCAATG(2/0) BssSI 557 CTCGTG CACGAG(-5/-1) BstAPI 967 GCAAGTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 371 CCAGCGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 396 GCTGAGC GC^T[ACGT]AGC DsaI 866 CCACGG C^C[GA][CT]GG EarI 1368 GAAGAG CTCTTC(1/4) EcoT14I 1344 CCAAGG C^C[AT][AT]GG FbaI 770 TGATCA T^GATCA Ksp632I 1368 GAAGAG CTCTTC(1/4) MspA1I 228 CAGCTG C[AC]G^C[GT]G PflMI 695 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 1214 GAGTC GAGTC(4/5) PmaCI 766 CACGTG CAC^GTG PmlI 766 CACGTG CAC^GTG PvuII 228 CAGCTG CAG^CTG SfiI 25 GGCCTCCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmlI 1208 CTCAAG C^T[CT][GA]AG StyI 1344 CCAAGG C^C[AT][AT]GG Tsp509I 1322 AATT ^AATT Van91I 695 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AfaI 102 GTAC GT^AC 1237 GTAC AflIII 469 ACATGT A^C[GA][CT]GT 1246 ACATGT AvaI 417 CCCGAG C^[CT]CG[GA]G 618 CTCGGG BalI 683 TGGCCA TGG^CCA 989 TGGCCA BcnI 365 CCGGG CC^[GC]GG 582 CCGGG BfaI 115 CTAG C^TAG 998 CTAG BpmI 1080 CTGGAG CTGGAG(16/14) 1102 CTGGAG BsaAI 766 CACGTG [CT]AC^GT[GA] 1328 TACGTG BsoBI 417 CCCGAG C^[CT]CG[GA]G 618 CTCGGG BspLU11I 469 ACATGT A^CATGT 1246 ACATGT BsrI 1358 ACTGG ACTGG(1/-1) 686 CCAGT CfoI 329 GCGC GCG^C 374 GCGC HhaI 329 GCGC GCG^C 374 GCGC HinP1I 329 GCGC G^CGC 374 GCGC MaeI 115 CTAG C^TAG 998 CTAG MluNI 683 TGGCCA TGG^CCA 989 TGGCCA MscI 683 TGGCCA TGG^CCA 989 TGGCCA MseI 1167 TTAA T^TAA 1420 TTAA NciI 365 CCGGG CC^[GC]GG 582 CCGGG NspI 469 ACATGT [GA]CATG^[CT] 1246 ACATGT RsaI 102 GTAC GT^AC 1237 GTAC StuI 24 AGGCCT AGG^CCT 440 AGGCCT Tru9I 1167 TTAA T^TAA 1420 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 57 enzymes) # ######################################### AciI AluI AlwI AlwNI AvaII BanII BbvI BmyI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MspI MvaI MwoI NdeII NlaIII NlaIV PpuMI Sau3AI Sau96I ScrFI SfaNI SfcI TfiI TseI Tsp45I TspRI XhoII