################################## # NO CUT ENZYMES ( 159 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbeI BbrPI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I CelII CfoI Cfr10I ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HhaI HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MaeIII MamI MfeI MflI MluI MroI MslI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tsp45I Tsp509I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AcyI 520 GACGCC G[GA]^CG[CT]C AocI 297 CCTGAGG CC^T[ACGT]AGG ApaI 203 GGGCCC GGGCC^C AvaI 716 CCCGAG C^[CT]CG[GA]G BalI 784 TGGCCA TGG^CCA BbsI 25 GAAGAC GAAGAC(2/6) BbvCI 484 CCTCAGC CCTCAGC(-5/-2) BcnI 332 CCCGG CC^[GC]GG BpmI 310 CTGGAG CTGGAG(16/14) BpuAI 25 GAAGAC GAAGAC(2/6) BsaHI 520 GACGCC G[GA]^CG[CT]C BsaI 818 GAGACC GGTCTC(1/5) BsoBI 716 CCCGAG C^[CT]CG[GA]G BspMI 580 ACCTGC ACCTGC(4/8) Bsu36I 297 CCTGAGG CC^T[ACGT]AGG DpnII 549 GATC ^GATC DraI 774 TTTAAA TTT^AAA DraIII 569 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 297 CCTGAGG CC^T[ACGT]AGG EcoNI 172 CCTTCAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 131 CCATGG C^C[AT][AT]GG FauI 323 CCCGC CCCGC(4/6) HapII 333 CCGG C^CGG HgaI 520 GACGC GACGC(5/10) Hin1I 520 GACGCC G[GA]^CG[CT]C HpaII 333 CCGG C^CGG HphI 546 GGTGA GGTGA(8/7) MaeII 17 ACGT A^CGT MboI 549 GATC ^GATC MluNI 784 TGGCCA TGG^CCA MscI 784 TGGCCA TGG^CCA MspI 333 CCGG C^CGG NciI 332 CCCGG CC^[GC]GG NcoI 131 CCATGG C^CATGG NdeII 549 GATC ^GATC NspI 702 ACATGC [GA]CATG^[CT] PleI 28 GACTC GAGTC(4/5) PpuMI 732 GGGTCCT [GA]G^G[AT]CC[CT] PvuII 277 CAGCTG CAG^CTG SacI 222 GAGCTC GAGCT^C SapI 843 GAAGAGC GCTCTTC(1/4) Sau3AI 549 GATC ^GATC SexAI 542 ACCTGGT A^CC[AT]GGT StyI 131 CCATGG C^C[AT][AT]GG TaqI 551 TCGA T^CGA TfiI 37 GATTC G^A[AT]TC TthHB8I 551 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AspHI 222 GAGCTC G[AT]GC[AT]^C 474 GAGCAC AvaII 355 GGACC G^G[AT]CC 733 GGTCC BanI 409 GGTGCC G^G[CT][GA]CC 588 GGCACC BsiHKAI 222 GAGCTC G[AT]GC[AT]^C 474 GAGCAC BsmAI 70 GAGAC GTCTC(1/5) 818 GAGAC DsaI 131 CCATGG C^C[GA][CT]GG 371 CCGTGG EarI 117 GAAGAG CTCTTC(1/4) 843 GAAGAG HinfI 28 GACTC G^A[ACGT]TC 37 GATTC Ksp632I 117 GAAGAG CTCTTC(1/4) 843 GAAGAG MseI 775 TTAA T^TAA 886 TTAA MspA1I 277 CAGCTG C[AC]G^C[GT]G 382 CCGCTG Tru9I 775 TTAA T^TAA 886 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 40 enzymes) # ######################################### AciI AluI BanII BbvI BmyI BsaJI BseRI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DraII EaeI EcoO109I EcoRII Fnu4HI FokI HaeIII ItaI MboII MnlI MvaI MwoI NlaIII NlaIV PstI Sau96I ScrFI SfaNI SfcI SmlI TseI TspRI