##################################
# NO CUT ENZYMES ( 159 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AfaI AflII AflIII
AgeI AhdI Alw44I AlwI AlwNI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BbeI BbrPI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I CelII
CfoI Cfr10I ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HhaI HinP1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeI MaeIII MamI
MfeI MflI MluI MroI MslI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tsp45I Tsp509I
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AcyI 520 GACGCC G[GA]^CG[CT]C
AocI 297 CCTGAGG CC^T[ACGT]AGG
ApaI 203 GGGCCC GGGCC^C
AvaI 716 CCCGAG C^[CT]CG[GA]G
BalI 784 TGGCCA TGG^CCA
BbsI 25 GAAGAC GAAGAC(2/6)
BbvCI 484 CCTCAGC CCTCAGC(-5/-2)
BcnI 332 CCCGG CC^[GC]GG
BpmI 310 CTGGAG CTGGAG(16/14)
BpuAI 25 GAAGAC GAAGAC(2/6)
BsaHI 520 GACGCC G[GA]^CG[CT]C
BsaI 818 GAGACC GGTCTC(1/5)
BsoBI 716 CCCGAG C^[CT]CG[GA]G
BspMI 580 ACCTGC ACCTGC(4/8)
Bsu36I 297 CCTGAGG CC^T[ACGT]AGG
DpnII 549 GATC ^GATC
DraI 774 TTTAAA TTT^AAA
DraIII 569 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 297 CCTGAGG CC^T[ACGT]AGG
EcoNI 172 CCTTCAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 131 CCATGG C^C[AT][AT]GG
FauI 323 CCCGC CCCGC(4/6)
HapII 333 CCGG C^CGG
HgaI 520 GACGC GACGC(5/10)
Hin1I 520 GACGCC G[GA]^CG[CT]C
HpaII 333 CCGG C^CGG
HphI 546 GGTGA GGTGA(8/7)
MaeII 17 ACGT A^CGT
MboI 549 GATC ^GATC
MluNI 784 TGGCCA TGG^CCA
MscI 784 TGGCCA TGG^CCA
MspI 333 CCGG C^CGG
NciI 332 CCCGG CC^[GC]GG
NcoI 131 CCATGG C^CATGG
NdeII 549 GATC ^GATC
NspI 702 ACATGC [GA]CATG^[CT]
PleI 28 GACTC GAGTC(4/5)
PpuMI 732 GGGTCCT [GA]G^G[AT]CC[CT]
PvuII 277 CAGCTG CAG^CTG
SacI 222 GAGCTC GAGCT^C
SapI 843 GAAGAGC GCTCTTC(1/4)
Sau3AI 549 GATC ^GATC
SexAI 542 ACCTGGT A^CC[AT]GGT
StyI 131 CCATGG C^C[AT][AT]GG
TaqI 551 TCGA T^CGA
TfiI 37 GATTC G^A[AT]TC
TthHB8I 551 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AspHI 222 GAGCTC G[AT]GC[AT]^C
474 GAGCAC
AvaII 355 GGACC G^G[AT]CC
733 GGTCC
BanI 409 GGTGCC G^G[CT][GA]CC
588 GGCACC
BsiHKAI 222 GAGCTC G[AT]GC[AT]^C
474 GAGCAC
BsmAI 70 GAGAC GTCTC(1/5)
818 GAGAC
DsaI 131 CCATGG C^C[GA][CT]GG
371 CCGTGG
EarI 117 GAAGAG CTCTTC(1/4)
843 GAAGAG
HinfI 28 GACTC G^A[ACGT]TC
37 GATTC
Ksp632I 117 GAAGAG CTCTTC(1/4)
843 GAAGAG
MseI 775 TTAA T^TAA
886 TTAA
MspA1I 277 CAGCTG C[AC]G^C[GT]G
382 CCGCTG
Tru9I 775 TTAA T^TAA
886 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AluI BanII BbvI BmyI
BsaJI BseRI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DraII EaeI
EcoO109I EcoRII Fnu4HI FokI HaeIII
ItaI MboII MnlI MvaI MwoI
NlaIII NlaIV PstI Sau96I ScrFI
SfaNI SfcI SmlI TseI TspRI