##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI AocI ApaI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AviII AvrII BamHI
BbrPI BcgI BclI BfrI BlnI
BsaBI BseAI BsiWI BspEI BspHI
BspLU11I BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CpoI DraI DraIII DrdI Eam1105I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HpaI KpnI KspI MamI MfeI
MluI MroI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SwaI TfiI XbaI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AflIII 1491 ACGTGT A^C[GA][CT]GT
Alw44I 758 GTGCAC G^TGCAC
AlwI 1209 GGATC GGATC(4/5)
ApaLI 758 GTGCAC G^TGCAC
AspI 1409 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 368 GGGCTC G[GA]GC[CT]^C
BbvCI 38 CCTCAGC CCTCAGC(-5/-2)
BciVI 110 GTATCC GTATCC(6/5)
BglI 1386 GCCACGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 462 AGATCT A^GATCT
BlpI 1009 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1009 GCTGAGC GC^T[ACGT]AGC
BsaWI 1482 ACCGGA [AT]^CCGG[AT]
BsiEI 1948 CGGCCG CG[GA][CT]^CG
BsmI 1159 GAATGC GAATGC(1/-1)
Bsp1407I 7 TGTACA T^GTACA
BspDI 581 ATCGAT AT^CGAT
BsrBI 995 GAGCGG CCGCTC(-3/-3)
BsrGI 7 TGTACA T^GTACA
BstYI 462 AGATCT [GA]^GATC[CT]
CelII 1009 GCTGAGC GC^T[ACGT]AGC
ClaI 581 ATCGAT AT^CGAT
DraII 1810 GGGTCCT [GA]G^G[ACGT]CC[CT]
EagI 1948 CGGCCG C^GGCCG
EclXI 1948 CGGCCG C^GGCCG
Eco52I 1948 CGGCCG C^GGCCG
EcoO109I 1810 GGGTCCT [GA]G^G[ACGT]CC[CT]
HindIII 324 AAGCTT A^AGCTT
MflI 462 AGATCT [GA]^GATC[CT]
MseI 2046 TTAA T^TAA
NcoI 1599 CCATGG C^CATGG
PaeR7I 1141 CTCGAG C^TCGAG
PflFI 1409 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1810 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 1913 GACCGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1741 CAGCTG CAG^CTG
SphI 315 GCATGC GCATG^C
SspBI 7 TGTACA T^GTACA
SspI 2049 AATATT AAT^ATT
StuI 1629 AGGCCT AGG^CCT
Tru9I 2046 TTAA T^TAA
Tth111I 1409 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 300 CCATGTTCACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1141 CTCGAG C^TCGAG
XhoII 462 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 665 GTCTAC GT^[AC][GT]AC
1415 GTCTAC
Aor51HI 1306 AGCGCT AGC^GCT
1605 AGCGCT
BbeI 34 GGCGCC GGCGC^C
1666 GGCGCC
BfaI 1031 CTAG C^TAG
1253 CTAG
BsaAI 362 TACGTG [CT]AC^GT[GA]
1355 TACGTG
BsaI 1322 GAGACC GGTCTC(1/5)
988 GGTCTC
BsmBI 1952 CGTCTC CGTCTC(1/5)
443 GAGACG
BsmFI 56 GGGAC GGGAC(10/14)
63 GGGAC
BsrDI 1123 CATTGC GCAATG(2/0)
1831 CATTGC
BsrFI 1072 GCCGGC [GA]^CCGG[CT]
1361 GCCGGC
Cfr10I 1072 GCCGGC [GA]^CCGG[CT]
1361 GCCGGC
DpnII 463 GATC ^GATC
1210 GATC
Eco47III 1306 AGCGCT AGC^GCT
1605 AGCGCT
KasI 34 GGCGCC G^GCGCC
1666 GGCGCC
MaeI 1031 CTAG C^TAG
1253 CTAG
MboI 463 GATC ^GATC
1210 GATC
MslI 643 CATCTCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1270 CATGCTGGTG
NaeI 1072 GCCGGC GCC^GGC
1361 GCCGGC
NarI 34 GGCGCC GG^CGCC
1666 GGCGCC
NdeII 463 GATC ^GATC
1210 GATC
NgoMIV 1072 GCCGGC G^CCGGC
1361 GCCGGC
PflMI 1341 CCAACTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1794 CCAACAGGTGG
SapI 239 GAAGAGC GCTCTTC(1/4)
970 GCTCTTC
Sau3AI 463 GATC ^GATC
1210 GATC
SfoI 34 GGCGCC GGC^GCC
1666 GGCGCC
Tsp509I 2044 AATT ^AATT
2061 AATT
Van91I 1341 CCAACTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1794 CCAACAGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AspHI AvaI AvaII BalI
BanI BbsI BbvI BcnI BmyI
BpmI BpuAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsoBI Bsp1286I BspMI BsrI BssKI
BstAPI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DsaI EaeI
EarI EcoNI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboII MluNI MnlI MscI
MspA1I MspI MvaI MvnI MwoI
NciI NlaIII NlaIV NspI PleI
PstI RsaI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TseI
Tsp45I TspRI TthHB8I