################################## # NO CUT ENZYMES ( 129 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BcgI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaWI BseAI BsiWI BsmI BspDI BspEI BsrBI BsrDI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRV EcoT22I FbaI FseI FspI HaeII HgaI Hin1I KasI KpnI MaeII MfeI MluI MroI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccI 612 GTCTAC GT^[AC][GT]AC AccII 141 CGCG CG^CG AflIII 616 ACATGT A^C[GA][CT]GT AocI 179 CCTCAGG CC^T[ACGT]AGG AvaI 926 CTCGGG C^[CT]CG[GA]G BanI 1191 GGTGCC G^G[CT][GA]CC BsaBI 566 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 26 CGACCG CG[GA][CT]^CG BsoBI 926 CTCGGG C^[CT]CG[GA]G BspLU11I 616 ACATGT A^CATGT BspMI 193 ACCTGC ACCTGC(4/8) BsrFI 1080 ACCGGC [GA]^CCGG[CT] BstUI 141 CGCG CG^CG BstYI 76 AGATCC [GA]^GATC[CT] Bsu36I 179 CCTCAGG CC^T[ACGT]AGG CfoI 1040 GCGC GCG^C Cfr10I 1080 ACCGGC [GA]^CCGG[CT] Eco81I 179 CCTCAGG CC^T[ACGT]AGG EcoNI 879 CCTGCTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 662 GAATTC G^AATTC FauI 139 CCCGC CCCGC(4/6) HhaI 1040 GCGC GCG^C HinP1I 1040 GCGC G^CGC HincII 1070 GTTAAC GT[CT]^[GA]AC HindII 1070 GTTAAC GT[CT]^[GA]AC HindIII 372 AAGCTT A^AGCTT HpaI 1070 GTTAAC GTT^AAC KspI 140 CCGCGG CCGC^GG MamI 566 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 76 AGATCC [GA]^GATC[CT] MvnI 141 CGCG CG^CG NcoI 670 CCATGG C^CATGG PstI 420 CTGCAG CTGCA^G SacII 140 CCGCGG CCGC^GG SapI 224 GCTCTTC GCTCTTC(1/4) SmlI 699 CTCAAG C^T[CT][GA]AG StuI 803 AGGCCT AGG^CCT TaqI 352 TCGA T^CGA TthHB8I 352 TCGA T^CGA XhoII 76 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AcsI 645 AAATTC [GA]^AATT[CT] 662 GAATTC AlwI 77 GATCC GGATC(4/5) 793 GATCC ApoI 645 AAATTC [GA]^AATT[CT] 662 GAATTC AspHI 222 GTGCTC G[AT]GC[AT]^C 597 GTGCTC BanII 341 GGGCTC G[GA]GC[CT]^C 960 GGGCTC BciVI 985 GGATAC GTATCC(6/5) 445 GTATCC BseRI 840 CTCCTC GAGGAG(10/8) 921 CTCCTC BsiHKAI 222 GTGCTC G[AT]GC[AT]^C 597 GTGCTC BsmBI 1005 CGTCTC CGTCTC(1/5) 1010 CGTCTC Bsp1407I 631 TGTACA T^GTACA 1224 TGTACA BspHI 97 TCATGA T^CATGA 1181 TCATGA BsrGI 631 TGTACA T^GTACA 1224 TGTACA BsrI 551 CCAGT ACTGG(1/-1) 1209 CCAGT DsaI 140 CCGCGG C^C[GA][CT]GG 670 CCATGG EarI 225 CTCTTC CTCTTC(1/4) 1113 GAAGAG EcoT14I 670 CCATGG C^C[AT][AT]GG 814 CCTTGG HphI 592 TCACC GGTGA(8/7) 953 TCACC Ksp632I 225 CTCTTC CTCTTC(1/4) 1113 GAAGAG PleI 749 GACTC GAGTC(4/5) 950 GAGTC PvuII 423 CAGCTG CAG^CTG 1158 CAGCTG RcaI 97 TCATGA T^CATGA 1181 TCATGA SspBI 631 TGTACA T^GTACA 1224 TGTACA StyI 670 CCATGG C^C[AT][AT]GG 814 CCTTGG TfiI 1118 GAATC G^A[AT]TC 1267 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 65 enzymes) # ######################################### AciI AfaI AluI AvaII BalI BbvI BcnI BfaI BmyI BpmI BsaJI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI MaeI MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI Tru9I TseI Tsp45I Tsp509I TspRI