##################################
# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Alw44I AlwNI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BbeI BbrPI
BbsI BbvCI BcgI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaHI BsaI BsaWI
BseAI BsiWI BsmI BspDI BspEI
BsrBI BsrDI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I CelII ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRV EcoT22I
FbaI FseI FspI HaeII HgaI
Hin1I KasI KpnI MaeII MfeI
MluI MroI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacI SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 612 GTCTAC GT^[AC][GT]AC
AccII 141 CGCG CG^CG
AflIII 616 ACATGT A^C[GA][CT]GT
AocI 179 CCTCAGG CC^T[ACGT]AGG
AvaI 926 CTCGGG C^[CT]CG[GA]G
BanI 1191 GGTGCC G^G[CT][GA]CC
BsaBI 566 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 26 CGACCG CG[GA][CT]^CG
BsoBI 926 CTCGGG C^[CT]CG[GA]G
BspLU11I 616 ACATGT A^CATGT
BspMI 193 ACCTGC ACCTGC(4/8)
BsrFI 1080 ACCGGC [GA]^CCGG[CT]
BstUI 141 CGCG CG^CG
BstYI 76 AGATCC [GA]^GATC[CT]
Bsu36I 179 CCTCAGG CC^T[ACGT]AGG
CfoI 1040 GCGC GCG^C
Cfr10I 1080 ACCGGC [GA]^CCGG[CT]
Eco81I 179 CCTCAGG CC^T[ACGT]AGG
EcoNI 879 CCTGCTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 662 GAATTC G^AATTC
FauI 139 CCCGC CCCGC(4/6)
HhaI 1040 GCGC GCG^C
HinP1I 1040 GCGC G^CGC
HincII 1070 GTTAAC GT[CT]^[GA]AC
HindII 1070 GTTAAC GT[CT]^[GA]AC
HindIII 372 AAGCTT A^AGCTT
HpaI 1070 GTTAAC GTT^AAC
KspI 140 CCGCGG CCGC^GG
MamI 566 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 76 AGATCC [GA]^GATC[CT]
MvnI 141 CGCG CG^CG
NcoI 670 CCATGG C^CATGG
PstI 420 CTGCAG CTGCA^G
SacII 140 CCGCGG CCGC^GG
SapI 224 GCTCTTC GCTCTTC(1/4)
SmlI 699 CTCAAG C^T[CT][GA]AG
StuI 803 AGGCCT AGG^CCT
TaqI 352 TCGA T^CGA
TthHB8I 352 TCGA T^CGA
XhoII 76 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 645 AAATTC [GA]^AATT[CT]
662 GAATTC
AlwI 77 GATCC GGATC(4/5)
793 GATCC
ApoI 645 AAATTC [GA]^AATT[CT]
662 GAATTC
AspHI 222 GTGCTC G[AT]GC[AT]^C
597 GTGCTC
BanII 341 GGGCTC G[GA]GC[CT]^C
960 GGGCTC
BciVI 985 GGATAC GTATCC(6/5)
445 GTATCC
BseRI 840 CTCCTC GAGGAG(10/8)
921 CTCCTC
BsiHKAI 222 GTGCTC G[AT]GC[AT]^C
597 GTGCTC
BsmBI 1005 CGTCTC CGTCTC(1/5)
1010 CGTCTC
Bsp1407I 631 TGTACA T^GTACA
1224 TGTACA
BspHI 97 TCATGA T^CATGA
1181 TCATGA
BsrGI 631 TGTACA T^GTACA
1224 TGTACA
BsrI 551 CCAGT ACTGG(1/-1)
1209 CCAGT
DsaI 140 CCGCGG C^C[GA][CT]GG
670 CCATGG
EarI 225 CTCTTC CTCTTC(1/4)
1113 GAAGAG
EcoT14I 670 CCATGG C^C[AT][AT]GG
814 CCTTGG
HphI 592 TCACC GGTGA(8/7)
953 TCACC
Ksp632I 225 CTCTTC CTCTTC(1/4)
1113 GAAGAG
PleI 749 GACTC GAGTC(4/5)
950 GAGTC
PvuII 423 CAGCTG CAG^CTG
1158 CAGCTG
RcaI 97 TCATGA T^CATGA
1181 TCATGA
SspBI 631 TGTACA T^GTACA
1224 TGTACA
StyI 670 CCATGG C^C[AT][AT]GG
814 CCTTGG
TfiI 1118 GAATC G^A[AT]TC
1267 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AfaI AluI AvaII BalI
BbvI BcnI BfaI BmyI BpmI
BsaJI BsgI BsiYI BslI BsmAI
BsmFI Bsp1286I BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraII
EaeI EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HinfI HpaII ItaI
MaeI MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PpuMI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
Tru9I TseI Tsp45I Tsp509I TspRI