##################################
# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AgeI AhdI Aor51HI ApaI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BanI BbeI
BbrPI BciVI BclI BfrI BlnI
BsaBI BsiEI BsiWI BsmBI BsmI
BspDI BspLU11I BsrDI BsrFI BssSI
Bst1107I BstBI BstZ17I Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HincII HindII HindIII HinfI
HpaI KasI KpnI KspI MamI
MfeI MseI MunI NaeI NarI
NdeI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SspI StuI SwaI TfiI
Tru9I Tsp509I XbaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccI 743 GTAGAC GT^[AC][GT]AC
AccIII 61 TCCGGA T^CCGGA
AcyI 338 GGCGTC G[GA]^CG[CT]C
AflIII 211 ACGCGT A^C[GA][CT]GT
Alw44I 672 GTGCAC G^TGCAC
AocI 842 CCTGAGG CC^T[ACGT]AGG
ApaLI 672 GTGCAC G^TGCAC
AspI 770 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 1033 GCTGAGG CCTCAGC(-5/-2)
BcgI 322 GCAGGGCACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 161 CCGGG CC^[GC]GG
BglII 1312 AGATCT A^GATCT
BlpI 1197 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1197 GCTCAGC GC^T[ACGT]AGC
BsaAI 1067 TACGTG [CT]AC^GT[GA]
BsaHI 338 GGCGTC G[GA]^CG[CT]C
BsaI 1291 GAGACC GGTCTC(1/5)
BsaWI 61 TCCGGA [AT]^CCGG[AT]
BseAI 61 TCCGGA T^CCGGA
BsgI 460 CTGCAC GTGCAG(16/14)
Bsp1407I 1244 TGTACA T^GTACA
BspEI 61 TCCGGA T^CCGGA
BspHI 480 TCATGA T^CATGA
BsrBI 410 GAGCGG CCGCTC(-3/-3)
BsrGI 1244 TGTACA T^GTACA
BstEII 1009 GGTTACC G^GT[ACGT]ACC
BstPI 1009 GGTTACC G^GT[ACGT]ACC
BstXI 868 CCAGGTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 842 CCTGAGG CC^T[ACGT]AGG
CelII 1197 GCTCAGC GC^T[ACGT]AGC
Eco81I 842 CCTGAGG CC^T[ACGT]AGG
EcoNI 1029 CCTTGCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1009 GGTTACC G^GT[ACGT]ACC
Hin1I 338 GGCGTC G[GA]^CG[CT]C
HphI 987 TCACC GGTGA(8/7)
MluI 211 ACGCGT A^CGCGT
MroI 61 TCCGGA T^CCGGA
MslI 751 CATCTTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 161 CCGGG CC^[GC]GG
PflFI 770 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 480 TCATGA T^CATGA
SacI 89 GAGCTC GAGCT^C
SbfI 1177 CCTGCAGG CCTGCA^GG
SfaNI 1483 GATGC GCATC(5/9)
Sse8387I 1177 CCTGCAGG CCTGCA^GG
SspBI 1244 TGTACA T^GTACA
TaqI 570 TCGA T^CGA
Tth111I 770 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 570 TCGA T^CGA
XcmI 990 CCAGAGGCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AlwNI 194 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
689 CAGATCCTG
AvaI 69 CCCGAG C^[CT]CG[GA]G
932 CTCGGG
BamHI 414 GGATCC G^GATCC
1575 GGATCC
BbsI 385 GAAGAC GAAGAC(2/6)
776 GTCTTC
BglI 50 GCCGACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1188 GCCTTGCTGGC
BpuAI 385 GAAGAC GAAGAC(2/6)
776 GTCTTC
BsoBI 69 CCCGAG C^[CT]CG[GA]G
932 CTCGGG
EarI 260 GAAGAG CTCTTC(1/4)
1119 GAAGAG
HgaI 110 GACGC GACGC(5/10)
339 GCGTC
Ksp632I 260 GAAGAG CTCTTC(1/4)
1119 GAAGAG
MaeII 558 ACGT A^CGT
1068 ACGT
PvuII 8 CAGCTG CAG^CTG
141 CAGCTG
SphI 579 GCATGC GCATG^C
1276 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaII BalI BanII BbvI
BfaI BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssHII BssKI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HpaII ItaI
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MspA1I MspI
MvaI MvnI MwoI NcoI NdeII
NheI NlaIII NlaIV NspI PflMI
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfcI StyI TseI Tsp45I
TspRI Van91I XhoII