################################## # NO CUT ENZYMES ( 112 enzymes) # ################################## AatII Acc65I AclI AcsI AflII AgeI AhdI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BanI BbeI BbrPI BciVI BclI BfrI BlnI BsaBI BsiEI BsiWI BsmBI BsmI BspDI BspLU11I BsrDI BsrFI BssSI Bst1107I BstBI BstZ17I Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HincII HindII HindIII HinfI HpaI KasI KpnI KspI MamI MfeI MseI MunI NaeI NarI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SspI StuI SwaI TfiI Tru9I Tsp509I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccI 743 GTAGAC GT^[AC][GT]AC AccIII 61 TCCGGA T^CCGGA AcyI 338 GGCGTC G[GA]^CG[CT]C AflIII 211 ACGCGT A^C[GA][CT]GT Alw44I 672 GTGCAC G^TGCAC AocI 842 CCTGAGG CC^T[ACGT]AGG ApaLI 672 GTGCAC G^TGCAC AspI 770 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC BbvCI 1033 GCTGAGG CCTCAGC(-5/-2) BcgI 322 GCAGGGCACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 161 CCGGG CC^[GC]GG BglII 1312 AGATCT A^GATCT BlpI 1197 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1197 GCTCAGC GC^T[ACGT]AGC BsaAI 1067 TACGTG [CT]AC^GT[GA] BsaHI 338 GGCGTC G[GA]^CG[CT]C BsaI 1291 GAGACC GGTCTC(1/5) BsaWI 61 TCCGGA [AT]^CCGG[AT] BseAI 61 TCCGGA T^CCGGA BsgI 460 CTGCAC GTGCAG(16/14) Bsp1407I 1244 TGTACA T^GTACA BspEI 61 TCCGGA T^CCGGA BspHI 480 TCATGA T^CATGA BsrBI 410 GAGCGG CCGCTC(-3/-3) BsrGI 1244 TGTACA T^GTACA BstEII 1009 GGTTACC G^GT[ACGT]ACC BstPI 1009 GGTTACC G^GT[ACGT]ACC BstXI 868 CCAGGTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 842 CCTGAGG CC^T[ACGT]AGG CelII 1197 GCTCAGC GC^T[ACGT]AGC Eco81I 842 CCTGAGG CC^T[ACGT]AGG EcoNI 1029 CCTTGCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1009 GGTTACC G^GT[ACGT]ACC Hin1I 338 GGCGTC G[GA]^CG[CT]C HphI 987 TCACC GGTGA(8/7) MluI 211 ACGCGT A^CGCGT MroI 61 TCCGGA T^CCGGA MslI 751 CATCTTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NciI 161 CCGGG CC^[GC]GG PflFI 770 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC RcaI 480 TCATGA T^CATGA SacI 89 GAGCTC GAGCT^C SbfI 1177 CCTGCAGG CCTGCA^GG SfaNI 1483 GATGC GCATC(5/9) Sse8387I 1177 CCTGCAGG CCTGCA^GG SspBI 1244 TGTACA T^GTACA TaqI 570 TCGA T^CGA Tth111I 770 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 570 TCGA T^CGA XcmI 990 CCAGAGGCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AlwNI 194 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG 689 CAGATCCTG AvaI 69 CCCGAG C^[CT]CG[GA]G 932 CTCGGG BamHI 414 GGATCC G^GATCC 1575 GGATCC BbsI 385 GAAGAC GAAGAC(2/6) 776 GTCTTC BglI 50 GCCGACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1188 GCCTTGCTGGC BpuAI 385 GAAGAC GAAGAC(2/6) 776 GTCTTC BsoBI 69 CCCGAG C^[CT]CG[GA]G 932 CTCGGG EarI 260 GAAGAG CTCTTC(1/4) 1119 GAAGAG HgaI 110 GACGC GACGC(5/10) 339 GCGTC Ksp632I 260 GAAGAG CTCTTC(1/4) 1119 GAAGAG MaeII 558 ACGT A^CGT 1068 ACGT PvuII 8 CAGCTG CAG^CTG 141 CAGCTG SphI 579 GCATGC GCATG^C 1276 GCATGC ######################################### # 3 OR MORE CUT ENZYMES ( 83 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AspHI AvaII BalI BanII BbvI BfaI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssHII BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII ItaI MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MspA1I MspI MvaI MvnI MwoI NcoI NdeII NheI NlaIII NlaIV NspI PflMI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfcI StyI TseI Tsp45I TspRI Van91I XhoII