##################################
# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Aor51HI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvrII BalI BamHI BbeI
BbrPI BcgI BclI BfaI BfrI
BglI BlnI BsaAI BsaBI BsaI
BsaWI BseAI BsiWI BsmAI BsmBI
Bsp1407I BspDI BspEI BspHI BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Cfr10I ClaI
CpoI DraI DraIII DrdI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
FbaI FseI HaeII HindIII HpaI
KasI KpnI Ksp632I KspI MaeI
MaeII MamI MfeI MluI MluNI
MroI MscI MunI NaeI NarI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI Tth111I XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AcsI 220 AAATTC [GA]^AATT[CT]
AcyI 463 GGCGTC G[GA]^CG[CT]C
AflIII 422 ACATGT A^C[GA][CT]GT
Alw44I 257 GTGCAC G^TGCAC
AocI 979 CCTGAGG CC^T[ACGT]AGG
ApaI 902 GGGCCC GGGCC^C
ApaLI 257 GTGCAC G^TGCAC
ApoI 220 AAATTC [GA]^AATT[CT]
AvaI 584 CCCGGG C^[CT]CG[GA]G
AviII 71 TGCGCA TGC^GCA
BanI 793 GGCACC G^G[CT][GA]CC
BbsI 1093 GAAGAC GAAGAC(2/6)
BlpI 964 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 964 GCTCAGC GC^T[ACGT]AGC
BpuAI 1093 GAAGAC GAAGAC(2/6)
BsaHI 463 GGCGTC G[GA]^CG[CT]C
BsgI 721 GTGCAG GTGCAG(16/14)
BsiEI 576 CGACCG CG[GA][CT]^CG
BsmFI 887 GGGAC GGGAC(10/14)
BsmI 637 GCATTC GAATGC(1/-1)
BsoBI 584 CCCGGG C^[CT]CG[GA]G
BspLU11I 422 ACATGT A^CATGT
BsrBI 883 GAGCGG CCGCTC(-3/-3)
BsrDI 677 GCAATG GCAATG(2/0)
BssSI 476 CTCGTG CACGAG(-5/-1)
Bsu36I 979 CCTGAGG CC^T[ACGT]AGG
CelII 964 GCTCAGC GC^T[ACGT]AGC
Eco81I 979 CCTGAGG CC^T[ACGT]AGG
EcoT22I 935 ATGCAT ATGCA^T
FspI 71 TGCGCA TGC^GCA
HgaI 464 GCGTC GACGC(5/10)
Hin1I 463 GGCGTC G[GA]^CG[CT]C
NdeI 152 CATATG CA^TATG
NsiI 935 ATGCAT ATGCA^T
PflMI 363 CCATGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1100 GACTC GAGTC(4/5)
ScaI 128 AGTACT AGT^ACT
SfcI 594 CTACAG C^T[GA][CT]AG
SmaI 584 CCCGGG CCC^GGG
SmlI 769 CTCAAG C^T[CT][GA]AG
SphI 519 GCATGC GCATG^C
TfiI 59 GAATC G^A[AT]TC
Van91I 363 CCATGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 941 CCAGGGAGGAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 584 CCCGGG C^CCGGG
XmaI 584 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 230 CGCG CG^CG
580 CGCG
AlwNI 597 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
1067 CAGCTGCTG
BanII 902 GGGCCC G[GA]GC[CT]^C
1030 GAGCCC
BbvCI 702 GCTGAGG CCTCAGC(-5/-2)
874 GCTGAGG
BciVI 684 GGATAC GTATCC(6/5)
707 GGATAC
BspMI 68 ACCTGC ACCTGC(4/8)
320 ACCTGC
BstUI 230 CGCG CG^CG
580 CGCG
DraII 350 GGGGCCT [GA]G^G[ACGT]CC[CT]
901 AGGGCCC
DsaI 368 CCATGG C^C[GA][CT]GG
1122 CCATGG
EcoO109I 350 GGGGCCT [GA]G^G[ACGT]CC[CT]
901 AGGGCCC
FauI 359 CCCGC CCCGC(4/6)
885 GCGGG
HapII 585 CCGG C^CGG
624 CCGG
HincII 36 GTCAAC GT[CT]^[GA]AC
745 GTCAAC
HindII 36 GTCAAC GT[CT]^[GA]AC
745 GTCAAC
HinfI 59 GAATC G^A[ACGT]TC
1100 GACTC
HpaII 585 CCGG C^CGG
624 CCGG
HphI 568 GGTGA GGTGA(8/7)
467 TCACC
MaeIII 466 GTCAC ^GT[ACGT]AC
1127 GTCAC
MseI 98 TTAA T^TAA
428 TTAA
MspI 585 CCGG C^CGG
624 CCGG
MvnI 230 CGCG CG^CG
580 CGCG
NcoI 368 CCATGG C^CATGG
1122 CCATGG
NspI 422 ACATGT [GA]CATG^[CT]
519 GCATGC
TaqI 284 TCGA T^CGA
575 TCGA
Tru9I 98 TTAA T^TAA
428 TTAA
Tsp45I 466 GTCAC ^GT[GC]AC
1127 GTCAC
Tsp509I 221 AATT ^AATT
950 AATT
TthHB8I 284 TCGA T^CGA
575 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BbvI BcnI BglII BmyI
BpmI BsaJI BseRI BsiHKAI BsiYI
BslI Bsp1286I BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII EcoRII EcoT14I
Fnu4HI FokI HaeIII HhaI HinP1I
ItaI MboI MboII MflI MnlI
MslI MspA1I MvaI MwoI NciI
NdeII NlaIII NlaIV PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI StyI
TseI TspRI XhoII