##################################
# NO CUT ENZYMES ( 156 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AflII AflIII AgeI Alw44I
AlwNI AocI ApaLI AscI AseI
AsnI Asp700I Asp718I AspHI AspI
AvaI AvaII AviII AvrII BalI
BanI BbeI BbrPI BbvCI BcgI
BciVI BcnI BfaI BfrI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaWI BseAI BseRI BsgI BsiHKAI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BsrI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI EaeI EagI EclXI Eco52I
Eco81I EcoO65I EcoT22I FseI FspI
Hin1I HindIII HpaI KasI KpnI
KspI MaeI MamI MfeI MluI
MluNI MroI MscI MseI MslI
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Tru9I Tsp45I Tth111I
Van91I XbaI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AclI 369 AACGTT AA^CGTT
AcsI 584 GAATTC [GA]^AATT[CT]
AfaI 676 GTAC GT^AC
AhdI 386 GACAATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 204 AGCGCT AGC^GCT
ApaI 304 GGGCCC GGGCC^C
ApoI 584 GAATTC [GA]^AATT[CT]
AspEI 386 GACAATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 735 GGATCC G^GATCC
BbsI 406 GAAGAC GAAGAC(2/6)
BclI 642 TGATCA T^GATCA
BglI 173 GCCAAGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 258 AGATCT A^GATCT
BpuAI 406 GAAGAC GAAGAC(2/6)
BsaI 128 GAGACC GGTCTC(1/5)
BsiEI 211 CGACCG CG[GA][CT]^CG
BsmI 296 GCATTC GAATGC(1/-1)
BstAPI 49 GCAGAGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 205 GCGC GCG^C
DraII 304 GGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 90 CCATGG C^C[GA][CT]GG
Eam1105I 386 GACAATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 320 GAAGAG CTCTTC(1/4)
Eco47III 204 AGCGCT AGC^GCT
EcoNI 623 CCTGAGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 304 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 584 GAATTC G^AATTC
EcoRV 773 GATATC GAT^ATC
FauI 399 GCGGG CCCGC(4/6)
FbaI 642 TGATCA T^GATCA
HaeII 204 AGCGCT [GA]GCGC^[CT]
HapII 376 CCGG C^CGG
HgaI 485 GACGC GACGC(5/10)
HhaI 205 GCGC GCG^C
HinP1I 205 GCGC G^CGC
HincII 541 GTCAAC GT[CT]^[GA]AC
HindII 541 GTCAAC GT[CT]^[GA]AC
HpaII 376 CCGG C^CGG
HphI 669 TCACC GGTGA(8/7)
Ksp632I 320 GAAGAG CTCTTC(1/4)
MaeII 370 ACGT A^CGT
MaeIII 279 GTAAC ^GT[ACGT]AC
MspA1I 62 CAGCGG C[AC]G^C[GT]G
MspI 376 CCGG C^CGG
NcoI 90 CCATGG C^CATGG
NspI 828 GCATGT [GA]CATG^[CT]
PleI 615 GAGTC GAGTC(4/5)
Psp1406I 369 AACGTT AA^CGTT
RsaI 676 GTAC GT^AC
SapI 320 GAAGAGC GCTCTTC(1/4)
SmlI 575 CTCAAG C^T[CT][GA]AG
SspI 852 AATATT AAT^ATT
TspRI 286 CAGTG CA[GC]TG[ACGT][ACGT]^
XcmI 90 CCATGGAAAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AciI 64 GCGG CCGC(-3/-1)
399 GCGG
BanII 304 GGGCCC G[GA]GC[CT]^C
323 GAGCCC
BpmI 26 CTCCAG CTGGAG(16/14)
101 CTGGAG
BsmAI 114 GAGAC GTCTC(1/5)
128 GAGAC
BsmFI 633 GGGAC GGGAC(10/14)
274 GTCCC
BstYI 258 AGATCT [GA]^GATC[CT]
735 GGATCC
Cfr13I 304 GGGCC G^G[ACGT]CC
305 GGCCC
MflI 258 AGATCT [GA]^GATC[CT]
735 GGATCC
NlaIV 304 GGGCCC GG[ACGT]^[ACGT]CC
735 GGATCC
Sau96I 304 GGGCC G^G[ACGT]CC
305 GGCCC
SfaNI 683 GATGC GCATC(5/9)
339 GCATC
TaqI 210 TCGA T^CGA
663 TCGA
TthHB8I 210 TCGA T^CGA
663 TCGA
XhoII 258 AGATCT [GA]^GATC[CT]
735 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AluI AlwI BbvI BmyI BsaJI
BsiYI BslI Bsp1286I BssKI BstF5I
BstNI Cac8I DdeI DpnII EcoRII
EcoT14I Fnu4HI FokI HaeIII HinfI
ItaI MboI MboII MnlI MvaI
MwoI NdeII NlaIII Sau3AI ScrFI
StyI TfiI TseI Tsp509I