##################################
# NO CUT ENZYMES ( 103 enzymes) #
##################################
Acc65I AccI AclI AcsI AflII
AgeI AhdI Alw44I Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvrII BciVI
BclI BfrI BglII BlnI BsaBI
BsiWI Bsp1407I BspDI BspLU11I BspMI
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI HindIII HpaI KpnI MamI
MfeI MluI MunI NcoI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PflFI PflMI PinAI
PmeI PshBI Psp1406I RsrII SacI
SalI SapI ScaI SexAI SfaNI
SfuI SgrAI SmaI SnaBI SpeI
SphI SspBI SspI StyI SwaI
Tsp45I Tsp509I Tth111I Van91I XbaI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 1243 GACGTC GACGT^C
AccIII 982 TCCGGA T^CCGGA
AflIII 811 ACGTGT A^C[GA][CT]GT
AocI 246 CCTAAGG CC^T[ACGT]AGG
ApaI 732 GGGCCC GGGCC^C
AspHI 514 GAGCAC G[AT]GC[AT]^C
BamHI 1493 GGATCC G^GATCC
BanII 732 GGGCCC G[GA]GC[CT]^C
BbeI 368 GGCGCC GGCGC^C
BbrPI 534 CACGTG CAC^GTG
BbsI 1338 GTCTTC GAAGAC(2/6)
BcgI 1238 GCAGAGACGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 1338 GTCTTC GAAGAC(2/6)
BsaI 196 GAGACC GGTCTC(1/5)
BsaWI 982 TCCGGA [AT]^CCGG[AT]
BseAI 982 TCCGGA T^CCGGA
BsiEI 567 CGATCG CG[GA][CT]^CG
BsiHKAI 514 GAGCAC G[AT]GC[AT]^C
BsmI 971 GAATGC GAATGC(1/-1)
BspEI 982 TCCGGA T^CCGGA
BspHI 58 TCATGA T^CATGA
BsrBI 1029 GAGCGG CCGCTC(-3/-3)
BstXI 608 CCAGGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 246 CCTAAGG CC^T[ACGT]AGG
DsaI 263 CCGCGG C^C[GA][CT]GG
Eco81I 246 CCTAAGG CC^T[ACGT]AGG
HincII 960 GTCAAC GT[CT]^[GA]AC
HindII 960 GTCAAC GT[CT]^[GA]AC
KasI 368 GGCGCC G^GCGCC
KspI 263 CCGCGG CCGC^GG
MaeIII 586 GTAAC ^GT[ACGT]AC
MroI 982 TCCGGA T^CCGGA
MseI 576 TTAA T^TAA
MslI 53 CATCGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 368 GGCGCC GG^CGCC
PaeR7I 9 CTCGAG C^TCGAG
PmaCI 534 CACGTG CAC^GTG
PmlI 534 CACGTG CAC^GTG
PshAI 798 GACACAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 567 CGATCG CGAT^CG
RcaI 58 TCATGA T^CATGA
SacII 263 CCGCGG CCGC^GG
SbfI 639 CCTGCAGG CCTGCA^GG
SfoI 368 GGCGCC GGC^GCC
Sse8387I 639 CCTGCAGG CCTGCA^GG
Tru9I 576 TTAA T^TAA
XhoI 9 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AviII 816 TGCGCA TGC^GCA
1100 TGCGCA
BalI 1072 TGGCCA TGG^CCA
1487 TGGCCA
BanI 368 GGCGCC G^G[CT][GA]CC
909 GGTGCC
BbvCI 870 GCTGAGG CCTCAGC(-5/-2)
887 GCTGAGG
BlpI 1046 GCTGAGC GC^T[ACGT]AGC
1265 GCTGAGC
Bpu1102I 1046 GCTGAGC GC^T[ACGT]AGC
1265 GCTGAGC
BsaAI 534 CACGTG [CT]AC^GT[GA]
810 TACGTG
BsgI 89 CTGCAC GTGCAG(16/14)
1358 CTGCAC
BsmBI 467 GAGACG CGTCTC(1/5)
1241 GAGACG
BsrFI 14 GCCGGC [GA]^CCGG[CT]
1258 GCCGGC
BstYI 76 AGATCC [GA]^GATC[CT]
1493 GGATCC
CelII 1046 GCTGAGC GC^T[ACGT]AGC
1265 GCTGAGC
Cfr10I 14 GCCGGC [GA]^CCGG[CT]
1258 GCCGGC
EaeI 1072 TGGCCA [CT]^GGCC[GA]
1487 TGGCCA
EcoNI 833 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1124 CCTGTTGGAGG
FspI 816 TGCGCA TGC^GCA
1100 TGCGCA
HaeII 368 GGCGCC [GA]GCGC^[CT]
1216 AGCGCC
MflI 76 AGATCC [GA]^GATC[CT]
1493 GGATCC
MluNI 1072 TGGCCA TGG^CCA
1487 TGGCCA
MscI 1072 TGGCCA TGG^CCA
1487 TGGCCA
NaeI 14 GCCGGC GCC^GGC
1258 GCCGGC
NgoMIV 14 GCCGGC G^CCGGC
1258 GCCGGC
PpuMI 1174 GGGACCT [GA]G^G[AT]CC[CT]
1374 AGGTCCC
SfiI 637 GGCCTGCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1064 GGCCATGCTGGCC
SmlI 9 CTCGAG C^T[CT][GA]AG
361 CTCAAG
StuI 779 AGGCCT AGG^CCT
1229 AGGCCT
TfiI 903 GAATC G^A[AT]TC
938 GATTC
TspRI 92 CACTG CA[GC]TG[ACGT][ACGT]^
1432 CAGTG
XcmI 400 CCACAACCCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1498 CCAGGCAAGGGATGG
XhoII 76 AGATCC [GA]^GATC[CT]
1493 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AvaI AvaII BbvI
BcnI BfaI BglI BmyI BpmI
BsaHI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI BstAPI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MboI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfcI
TaqI TseI TthHB8I