##################################
# NO CUT ENZYMES ( 167 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI Asp700I
Asp718I AspEI AvaI AviII AvrII
BalI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BglI
BglII BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII CfoI ClaI
CpoI DraI DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HphI KasI
KpnI KspI MaeIII MamI MfeI
MluI MluNI MroI MscI MslI
MunI MvnI NarI NciI NdeI
NheI NotI NruI NspI NspV
PacI PaeR7I PflMI PinAI PleI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI Tsp45I
TthHB8I Van91I XbaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AciI 640 GCGG CCGC(-3/-1)
AflII 18 CTTAAG C^TTAAG
AseI 818 ATTAAT AT^TAAT
AsnI 818 ATTAAT AT^TAAT
AspHI 549 GAGCAC G[AT]GC[AT]^C
AspI 377 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 351 GGATCC G^GATCC
BanII 684 GGGCTC G[GA]GC[CT]^C
BbsI 312 GAAGAC GAAGAC(2/6)
BfrI 18 CTTAAG C^TTAAG
BpuAI 312 GAAGAC GAAGAC(2/6)
BsiHKAI 549 GAGCAC G[AT]GC[AT]^C
BsmBI 382 CGTCTC CGTCTC(1/5)
BstYI 351 GGATCC [GA]^GATC[CT]
DsaI 777 CCATGG C^C[GA][CT]GG
EcoT22I 678 ATGCAT ATGCA^T
HinfI 769 GAATC G^A[ACGT]TC
MaeII 381 ACGT A^CGT
MflI 351 GGATCC [GA]^GATC[CT]
MspA1I 638 CAGCGG C[AC]G^C[GT]G
NaeI 463 GCCGGC GCC^GGC
NcoI 777 CCATGG C^CATGG
NgoMIV 463 GCCGGC G^CCGGC
NsiI 678 ATGCAT ATGCA^T
PflFI 377 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 484 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 818 ATTAAT AT^TAAT
SfaNI 724 GATGC GCATC(5/9)
SmlI 18 CTTAAG C^T[CT][GA]AG
TfiI 769 GAATC G^A[AT]TC
Tth111I 377 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 520 CCATGTATCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 351 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AfaI 448 GTAC GT^AC
782 GTAC
AlwI 352 GATCC GGATC(4/5)
351 GGATC
BmyI 549 GAGCAC G[AGT]GC[ACT]^C
684 GGGCTC
BsaJI 668 CCAAGG C^C[ACGT][ACGT]GG
777 CCATGG
BsiYI 379 CCACGTCTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
476 CCAGACTGAGG
BslI 379 CCACGTCTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
476 CCAGACTGAGG
BsmAI 802 GAGAC GTCTC(1/5)
383 GTCTC
Bsp1286I 549 GAGCAC G[AGT]GC[ACT]^C
684 GGGCTC
BsrFI 463 GCCGGC [GA]^CCGG[CT]
553 ACCGGC
BssKI 152 CCAGG ^CC[ACGT]GG
471 CCAGG
BstF5I 23 GGATG GGATG(2/0)
319 GGATG
BstNI 152 CCAGG CC^[AT]GG
471 CCAGG
Cfr10I 463 GCCGGC [GA]^CCGG[CT]
553 ACCGGC
EcoRII 152 CCAGG ^CC[AT]GG
471 CCAGG
EcoT14I 668 CCAAGG C^C[AT][AT]GG
777 CCATGG
FokI 23 GGATG GGATG(9/13)
319 GGATG
HapII 464 CCGG C^CGG
554 CCGG
HpaII 464 CCGG C^CGG
554 CCGG
MseI 19 TTAA T^TAA
819 TTAA
MspI 464 CCGG C^CGG
554 CCGG
MvaI 152 CCAGG CC^[AT]GG
471 CCAGG
PstI 402 CTGCAG CTGCA^G
699 CTGCAG
RsaI 448 GTAC GT^AC
782 GTAC
SapI 129 GAAGAGC GCTCTTC(1/4)
704 GCTCTTC
ScrFI 152 CCAGG CC^[ACGT]GG
471 CCAGG
SfcI 402 CTGCAG C^T[GA][CT]AG
699 CTGCAG
StyI 668 CCAAGG C^C[AT][AT]GG
777 CCATGG
Tru9I 19 TTAA T^TAA
819 TTAA
Tsp509I 510 AATT ^AATT
821 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AluI AvaII BbvI BfaI BseRI
BsrI Cac8I Cfr13I DdeI DpnII
DraII EarI EcoO109I Fnu4HI HaeIII
ItaI Ksp632I MaeI MboI MboII
MnlI MwoI NdeII NlaIII NlaIV
Sau3AI Sau96I TseI TspRI