##################################
# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AflII AgeI Aor51HI ApaI
AscI AseI AsnI Asp700I AspI
AviII BanII BbeI BbrPI BciVI
BclI BfrI BglI BsaAI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BsrBI BsrFI BsrGI BssHII Bst1107I
BstAPI BstUI BstXI BstZ17I CfoI
Cfr10I ClaI CpoI DraI DrdI
DsaI EagI EclXI Eco47III Eco52I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HhaI Hin1I
HinP1I HindIII HpaI KasI KspI
MaeII MfeI MluI MroI MseI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI SwaI TfiI Tru9I Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
Acc65I 268 GGTACC G^GTACC
AflIII 1258 ACATGT A^C[GA][CT]GT
AhdI 813 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1085 CCTGAGG CC^T[ACGT]AGG
Asp718I 268 GGTACC G^GTACC
AspEI 813 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 823 CCCGAG C^[CT]CG[GA]G
AvrII 304 CCTAGG C^CTAGG
BalI 647 TGGCCA TGG^CCA
BamHI 900 GGATCC G^GATCC
BanI 268 GGTACC G^G[CT][GA]CC
BbsI 1023 GTCTTC GAAGAC(2/6)
BbvCI 153 GCTGAGG CCTCAGC(-5/-2)
BcgI 659 GCAACGAAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 272 CCGGG CC^[GC]GG
BglII 922 AGATCT A^GATCT
BlnI 304 CCTAGG C^CTAGG
BlpI 166 GCTGAGC GC^T[ACGT]AGC
BpmI 878 CTCCAG CTGGAG(16/14)
Bpu1102I 166 GCTGAGC GC^T[ACGT]AGC
BpuAI 1023 GTCTTC GAAGAC(2/6)
BsaBI 421 GATCTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 465 CTCCTC GAGGAG(10/8)
BsgI 71 CTGCAC GTGCAG(16/14)
BsmAI 691 GAGAC GTCTC(1/5)
BsmI 1379 GCATTC GAATGC(1/-1)
BsoBI 823 CCCGAG C^[CT]CG[GA]G
BspLU11I 1258 ACATGT A^CATGT
BsrDI 146 GCAATG GCAATG(2/0)
BssSI 1092 CTCGTG CACGAG(-5/-1)
BstEII 1306 GGTGACC G^GT[ACGT]ACC
BstPI 1306 GGTGACC G^GT[ACGT]ACC
Bsu36I 1085 CCTGAGG CC^T[ACGT]AGG
CelII 166 GCTGAGC GC^T[ACGT]AGC
DraII 619 AGGGCCT [GA]G^G[ACGT]CC[CT]
EaeI 647 TGGCCA [CT]^GGCC[GA]
Eam1105I 813 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1085 CCTGAGG CC^T[ACGT]AGG
EcoNI 381 CCTGTGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 619 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1306 GGTGACC G^GT[ACGT]ACC
EcoT14I 304 CCTAGG C^C[AT][AT]GG
HgaI 863 GCGTC GACGC(5/10)
KpnI 268 GGTACC GGTAC^C
MamI 421 GATCTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 647 TGGCCA TGG^CCA
MscI 647 TGGCCA TGG^CCA
NciI 272 CCGGG CC^[GC]GG
PshAI 1016 GACAGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 209 CTGCAG CTGCA^G
PvuII 1184 CAGCTG CAG^CTG
SphI 1172 GCATGC GCATG^C
StuI 179 AGGCCT AGG^CCT
StyI 304 CCTAGG C^C[AT][AT]GG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 540 AAATTC [GA]^AATT[CT]
1112 AAATTC
Alw44I 232 GTGCAC G^TGCAC
298 GTGCAC
AlwNI 248 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
500 CAGTGACTG
ApaLI 232 GTGCAC G^TGCAC
298 GTGCAC
ApoI 540 AAATTC [GA]^AATT[CT]
1112 AAATTC
AspHI 232 GTGCAC G[AT]GC[AT]^C
298 GTGCAC
BfaI 263 CTAG C^TAG
305 CTAG
BsiHKAI 232 GTGCAC G[AT]GC[AT]^C
298 GTGCAC
BsrI 353 ACTGG ACTGG(1/-1)
880 CCAGT
BstBI 1343 TTCGAA TT^CGAA
1382 TTCGAA
EarI 835 GAAGAG CTCTTC(1/4)
1328 GAAGAG
HapII 63 CCGG C^CGG
272 CCGG
HincII 670 GTTGAC GT[CT]^[GA]AC
1272 GTCAAC
HindII 670 GTTGAC GT[CT]^[GA]AC
1272 GTCAAC
HpaII 63 CCGG C^CGG
272 CCGG
Ksp632I 835 GAAGAG CTCTTC(1/4)
1328 GAAGAG
MaeI 263 CTAG C^TAG
305 CTAG
MslI 1286 CACTCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1300 CATGGGGGTG
MspI 63 CCGG C^CGG
272 CCGG
NspV 1343 TTCGAA TT^CGAA
1382 TTCGAA
SfuI 1343 TTCGAA TT^CGAA
1382 TTCGAA
SmlI 562 CTCAAG C^T[CT][GA]AG
906 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BbvI BmyI BsaJI BsiYI BslI
BsmFI Bsp1286I BspMI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraIII EcoRII Fnu4HI FokI
HaeIII HinfI HphI ItaI MaeIII
MboI MboII MflI MnlI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII