##################################
# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BbeI
BcgI BciVI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BseAI
BsiEI BsiWI BspEI BspHI BspLU11I
BspMI BsrDI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I CelII CpoI DraI DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KasI KpnI KspI
MfeI MluI MluNI MroI MscI
MslI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI SwaI Tth111I
XbaI XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AgeI 257 ACCGGT A^CCGGT
AocI 1309 CCTGAGG CC^T[ACGT]AGG
AspHI 974 GAGCTC G[AT]GC[AT]^C
BbrPI 487 CACGTG CAC^GTG
BbsI 457 GAAGAC GAAGAC(2/6)
BpuAI 457 GAAGAC GAAGAC(2/6)
BsaAI 487 CACGTG [CT]AC^GT[GA]
BsaBI 330 GATGGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 257 ACCGGT [AT]^CCGG[AT]
BsiHKAI 974 GAGCTC G[AT]GC[AT]^C
BsmI 1132 GCATTC GAATGC(1/-1)
Bsp1407I 820 TGTACA T^GTACA
BspDI 1422 ATCGAT AT^CGAT
BsrBI 523 GAGCGG CCGCTC(-3/-3)
BsrGI 820 TGTACA T^GTACA
Bsu36I 1309 CCTGAGG CC^T[ACGT]AGG
ClaI 1422 ATCGAT AT^CGAT
Eco81I 1309 CCTGAGG CC^T[ACGT]AGG
EcoRI 469 GAATTC G^AATTC
EcoRV 334 GATATC GAT^ATC
FauI 590 GCGGG CCCGC(4/6)
MamI 330 GATGGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PflMI 1452 CCAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 257 ACCGGT A^CCGGT
PmaCI 487 CACGTG CAC^GTG
PmlI 487 CACGTG CAC^GTG
PpuMI 1391 GGGTCCT [GA]G^G[AT]CC[CT]
PvuII 813 CAGCTG CAG^CTG
SacI 974 GAGCTC GAGCT^C
SapI 1033 GAAGAGC GCTCTTC(1/4)
SmaI 1521 CCCGGG CCC^GGG
SmlI 382 CTCAAG C^T[CT][GA]AG
SspBI 820 TGTACA T^GTACA
StuI 1108 AGGCCT AGG^CCT
TfiI 751 GAATC G^A[AT]TC
Van91I 1452 CCAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 1521 CCCGGG C^CCGGG
XmaI 1521 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AciI 525 GCGG CCGC(-3/-1)
590 GCGG
AcsI 469 GAATTC [GA]^AATT[CT]
1164 AAATTC
AcyI 1212 GACGCC G[GA]^CG[CT]C
1315 GACGCC
AlwI 1008 GATCC GGATC(4/5)
889 GGATC
ApoI 469 GAATTC [GA]^AATT[CT]
1164 AAATTC
AvaI 1188 CTCGGG C^[CT]CG[GA]G
1521 CCCGGG
BanI 910 GGCACC G^G[CT][GA]CC
1250 GGCACC
BsaHI 1212 GACGCC G[GA]^CG[CT]C
1315 GACGCC
BsmBI 289 GAGACG CGTCTC(1/5)
905 GAGACG
BsoBI 1188 CTCGGG C^[CT]CG[GA]G
1521 CCCGGG
BsrFI 121 ACCGGC [GA]^CCGG[CT]
257 ACCGGT
BssSI 507 CACGAG CACGAG(-5/-1)
971 CACGAG
BstYI 889 GGATCT [GA]^GATC[CT]
1007 AGATCC
Cfr10I 121 ACCGGC [GA]^CCGG[CT]
257 ACCGGT
DraII 641 GGGGCCT [GA]G^G[ACGT]CC[CT]
1391 GGGTCCT
EcoNI 231 CCTGCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
645 CCTGGGGGAGG
EcoO109I 641 GGGGCCT [GA]G^G[ACGT]CC[CT]
1391 GGGTCCT
HaeII 1063 AGCGCC [GA]GCGC^[CT]
1517 AGCGCC
Hin1I 1212 GACGCC G[GA]^CG[CT]C
1315 GACGCC
MaeII 398 ACGT A^CGT
488 ACGT
MflI 889 GGATCT [GA]^GATC[CT]
1007 AGATCC
MspA1I 588 CAGCGG C[AC]G^C[GT]G
813 CAGCTG
PstI 1240 CTGCAG CTGCA^G
1562 CTGCAG
TaqI 1168 TCGA T^CGA
1423 TCGA
TthHB8I 1168 TCGA T^CGA
1423 TCGA
XhoII 889 GGATCT [GA]^GATC[CT]
1007 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AfaI AluI AlwNI AvaII BanII
BbvCI BbvI BcnI BfaI BmyI
BpmI BsaI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MnlI MseI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
Tru9I TseI Tsp45I Tsp509I TspRI