##################################
# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI AspEI AspI AvrII
BalI BbeI BbrPI BbsI BbvCI
BcgI BciVI BfrI BlnI BlpI
Bpu1102I BpuAI BsaAI BsaHI BsaWI
BseAI BsgI BsiEI BsiWI BsmI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoRI EcoRV EcoT22I
FseI HgaI Hin1I HpaI KasI
KspI MaeII MfeI MluI MluNI
MroI MscI MunI MvnI NarI
NcoI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tth111I TthHB8I Van91I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 235 GGTACC G^GTACC
AcsI 745 AAATTT [GA]^AATT[CT]
AlwNI 211 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 109 GGGCCC GGGCC^C
ApoI 745 AAATTT [GA]^AATT[CT]
Asp700I 1253 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 235 GGTACC G^GTACC
AvaI 883 CCCGGG C^[CT]CG[GA]G
AviII 523 TGCGCA TGC^GCA
BanI 235 GGTACC G^G[CT][GA]CC
BclI 949 TGATCA T^GATCA
BglI 666 GCCTGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 1293 GATTTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 279 CGTCTC CGTCTC(1/5)
BsoBI 883 CCCGGG C^[CT]CG[GA]G
BspHI 64 TCATGA T^CATGA
BspMI 998 ACCTGC ACCTGC(4/8)
BsrDI 431 CATTGC GCAATG(2/0)
BsrFI 556 GCCGGC [GA]^CCGG[CT]
BstXI 44 CCAACCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 556 GCCGGC [GA]^CCGG[CT]
EarI 480 GAAGAG CTCTTC(1/4)
EcoT14I 529 CCAAGG C^C[AT][AT]GG
FbaI 949 TGATCA T^GATCA
FspI 523 TGCGCA TGC^GCA
HaeII 1271 GGCGCT [GA]GCGC^[CT]
HindIII 619 AAGCTT A^AGCTT
KpnI 235 GGTACC GGTAC^C
Ksp632I 480 GAAGAG CTCTTC(1/4)
MamI 1293 GATTTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 148 CACGGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 556 GCCGGC GCC^GGC
NgoMIV 556 GCCGGC G^CCGGC
RcaI 64 TCATGA T^CATGA
SmaI 883 CCCGGG CCC^GGG
SmlI 333 CTCAAG C^T[CT][GA]AG
StyI 529 CCAAGG C^C[AT][AT]GG
TfiI 638 GATTC G^A[AT]TC
XcmI 713 CCATCCCATTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 883 CCCGGG C^CCGGG
XmaI 883 CCCGGG C^CCGGG
XmnI 1253 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
BamHI 56 GGATCC G^GATCC
1163 GGATCC
BglII 253 AGATCT A^GATCT
337 AGATCT
BsaI 363 GAGACC GGTCTC(1/5)
423 GAGACC
BstEII 562 GGTGACC G^GT[ACGT]ACC
777 GGTGACC
BstPI 562 GGTGACC G^GT[ACGT]ACC
777 GGTGACC
EcoNI 296 CCTTGTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
754 CCTAACTCAGG
EcoO65I 562 GGTGACC G^GT[ACGT]ACC
777 GGTGACC
FauI 115 CCCGC CCCGC(4/6)
517 GCGGG
HincII 141 GTCAAC GT[CT]^[GA]AC
186 GTCAAC
HindII 141 GTCAAC GT[CT]^[GA]AC
186 GTCAAC
MseI 867 TTAA T^TAA
1194 TTAA
PleI 549 GAGTC GAGTC(4/5)
628 GAGTC
SacI 389 GAGCTC GAGCT^C
1330 GAGCTC
SfaNI 204 GATGC GCATC(5/9)
793 GCATC
Tru9I 867 TTAA T^TAA
1194 TTAA
TspRI 122 CAGTG CA[GC]TG[ACGT][ACGT]^
137 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BanII BbvI BcnI BfaI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MaeIII MboI MboII MflI MnlI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfcI TseI Tsp45I Tsp509I XhoII