################################## # NO CUT ENZYMES ( 130 enzymes) # ################################## AatII AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI AspEI AspI AvrII BalI BbeI BbrPI BbsI BbvCI BcgI BciVI BfrI BlnI BlpI Bpu1102I BpuAI BsaAI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT22I FseI HgaI Hin1I HpaI KasI KspI MaeII MfeI MluI MluNI MroI MscI MunI MvnI NarI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tth111I TthHB8I Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### Acc65I 235 GGTACC G^GTACC AcsI 745 AAATTT [GA]^AATT[CT] AlwNI 211 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 109 GGGCCC GGGCC^C ApoI 745 AAATTT [GA]^AATT[CT] Asp700I 1253 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 235 GGTACC G^GTACC AvaI 883 CCCGGG C^[CT]CG[GA]G AviII 523 TGCGCA TGC^GCA BanI 235 GGTACC G^G[CT][GA]CC BclI 949 TGATCA T^GATCA BglI 666 GCCTGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 1293 GATTTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmBI 279 CGTCTC CGTCTC(1/5) BsoBI 883 CCCGGG C^[CT]CG[GA]G BspHI 64 TCATGA T^CATGA BspMI 998 ACCTGC ACCTGC(4/8) BsrDI 431 CATTGC GCAATG(2/0) BsrFI 556 GCCGGC [GA]^CCGG[CT] BstXI 44 CCAACCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 556 GCCGGC [GA]^CCGG[CT] EarI 480 GAAGAG CTCTTC(1/4) EcoT14I 529 CCAAGG C^C[AT][AT]GG FbaI 949 TGATCA T^GATCA FspI 523 TGCGCA TGC^GCA HaeII 1271 GGCGCT [GA]GCGC^[CT] HindIII 619 AAGCTT A^AGCTT KpnI 235 GGTACC GGTAC^C Ksp632I 480 GAAGAG CTCTTC(1/4) MamI 1293 GATTTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MslI 148 CACGGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 556 GCCGGC GCC^GGC NgoMIV 556 GCCGGC G^CCGGC RcaI 64 TCATGA T^CATGA SmaI 883 CCCGGG CCC^GGG SmlI 333 CTCAAG C^T[CT][GA]AG StyI 529 CCAAGG C^C[AT][AT]GG TfiI 638 GATTC G^A[AT]TC XcmI 713 CCATCCCATTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 883 CCCGGG C^CCGGG XmaI 883 CCCGGG C^CCGGG XmnI 1253 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### BamHI 56 GGATCC G^GATCC 1163 GGATCC BglII 253 AGATCT A^GATCT 337 AGATCT BsaI 363 GAGACC GGTCTC(1/5) 423 GAGACC BstEII 562 GGTGACC G^GT[ACGT]ACC 777 GGTGACC BstPI 562 GGTGACC G^GT[ACGT]ACC 777 GGTGACC EcoNI 296 CCTTGTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 754 CCTAACTCAGG EcoO65I 562 GGTGACC G^GT[ACGT]ACC 777 GGTGACC FauI 115 CCCGC CCCGC(4/6) 517 GCGGG HincII 141 GTCAAC GT[CT]^[GA]AC 186 GTCAAC HindII 141 GTCAAC GT[CT]^[GA]AC 186 GTCAAC MseI 867 TTAA T^TAA 1194 TTAA PleI 549 GAGTC GAGTC(4/5) 628 GAGTC SacI 389 GAGCTC GAGCT^C 1330 GAGCTC SfaNI 204 GATGC GCATC(5/9) 793 GCATC Tru9I 867 TTAA T^TAA 1194 TTAA TspRI 122 CAGTG CA[GC]TG[ACGT][ACGT]^ 137 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 70 enzymes) # ######################################### AciI AfaI AluI AlwI AspHI AvaII BanII BbvI BcnI BfaI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfcI TseI Tsp45I Tsp509I XhoII