##################################
# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII AccIII AclI AcsI AflII
AflIII AgeI AhdI AocI Aor51HI
ApoI AscI AseI AsnI Asp700I
AspEI BalI BbeI BbrPI BcgI
BclI BfrI BlpI Bpu1102I BsaAI
BsaBI BsaWI BseAI BsiWI BsmBI
BsmI BspDI BspEI BspHI BspLU11I
BsrBI BssHII Bst1107I BstEII BstPI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV FbaI FseI HaeII HindIII
HpaI KasI KspI MaeII MamI
MfeI MluI MluNI MroI MscI
MslI MunI NaeI NarI NdeI
NgoMIV NotI NruI PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SfoI SgrAI SnaBI
SpeI Sse8387I SspI SwaI XbaI
XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 1596 GGTACC G^GTACC
AccI 646 GTCTAC GT^[AC][GT]AC
AccII 835 CGCG CG^CG
AcyI 712 GGCGTC G[GA]^CG[CT]C
Asp718I 1596 GGTACC G^GTACC
AspI 258 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 788 TGCGCA TGC^GCA
AvrII 93 CCTAGG C^CTAGG
BamHI 1019 GGATCC G^GATCC
BbvCI 311 CCTCAGC CCTCAGC(-5/-2)
BglII 635 AGATCT A^GATCT
BlnI 93 CCTAGG C^CTAGG
BsaHI 712 GGCGTC G[GA]^CG[CT]C
BsaI 427 GAGACC GGTCTC(1/5)
BsiEI 763 CGGTCG CG[GA][CT]^CG
Bsp1407I 689 TGTACA T^GTACA
BsrDI 1535 CATTGC GCAATG(2/0)
BsrFI 539 ACCGGC [GA]^CCGG[CT]
BsrGI 689 TGTACA T^GTACA
BssSI 683 CACGAG CACGAG(-5/-1)
BstBI 1038 TTCGAA TT^CGAA
BstUI 835 CGCG CG^CG
Cfr10I 539 ACCGGC [GA]^CCGG[CT]
DraIII 1331 CACTTAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 16 GACACTGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 541 CGGCCA [CT]^GGCC[GA]
EcoT22I 595 ATGCAT ATGCA^T
FspI 788 TGCGCA TGC^GCA
HgaI 713 GCGTC GACGC(5/10)
Hin1I 712 GGCGTC G[GA]^CG[CT]C
HincII 188 GTCAAC GT[CT]^[GA]AC
HindII 188 GTCAAC GT[CT]^[GA]AC
KpnI 1596 GGTACC GGTAC^C
MseI 1462 TTAA T^TAA
MvnI 835 CGCG CG^CG
NheI 1026 GCTAGC G^CTAGC
NsiI 595 ATGCAT ATGCA^T
NspV 1038 TTCGAA TT^CGAA
PflFI 258 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
ScaI 783 AGTACT AGT^ACT
SfuI 1038 TTCGAA TT^CGAA
SmlI 520 CTCAAG C^T[CT][GA]AG
SphI 889 GCATGC GCATG^C
SspBI 689 TGTACA T^GTACA
Tru9I 1462 TTAA T^TAA
Tsp509I 1158 AATT ^AATT
Tth111I 258 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Alw44I 973 GTGCAC G^TGCAC
1321 GTGCAC
ApaLI 973 GTGCAC G^TGCAC
1321 GTGCAC
AspHI 973 GTGCAC G[AT]GC[AT]^C
1321 GTGCAC
AvaI 406 CCCGGG C^[CT]CG[GA]G
1211 CCCGGG
BanI 379 GGCACC G^G[CT][GA]CC
1596 GGTACC
BbsI 255 GAAGAC GAAGAC(2/6)
958 GTCTTC
BciVI 553 GGATAC GTATCC(6/5)
1385 GTATCC
BglI 296 GCCGCAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1188 GCCACCACGGC
BpuAI 255 GAAGAC GAAGAC(2/6)
958 GTCTTC
BsgI 631 GTGCAG GTGCAG(16/14)
1165 GTGCAG
BsiHKAI 973 GTGCAC G[AT]GC[AT]^C
1321 GTGCAC
BsoBI 406 CCCGGG C^[CT]CG[GA]G
1211 CCCGGG
BstAPI 501 GCAGGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
884 GCACAGCATGC
EarI 754 CTCTTC CTCTTC(1/4)
952 GAAGAG
FauI 414 CCCGC CCCGC(4/6)
1076 CCCGC
HphI 172 GGTGA GGTGA(8/7)
481 TCACC
Ksp632I 754 CTCTTC CTCTTC(1/4)
952 GAAGAG
MspA1I 943 CAGCTG C[AC]G^C[GT]G
1114 CAGCTG
NcoI 1227 CCATGG C^CATGG
1602 CCATGG
PvuII 943 CAGCTG CAG^CTG
1114 CAGCTG
SmaI 406 CCCGGG CCC^GGG
1211 CCCGGG
StuI 524 AGGCCT AGG^CCT
614 AGGCCT
XmaCI 406 CCCGGG C^CCGGG
1211 CCCGGG
XmaI 406 CCCGGG C^CCGGG
1211 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
ApaI AvaII BanII BbvI BcnI
BfaI BmyI BpmI BsaJI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EcoNI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
ItaI MaeI MaeIII MboI MboII
MflI MnlI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI TseI Tsp45I
TspRI TthHB8I Van91I XhoII