################################## # NO CUT ENZYMES ( 108 enzymes) # ################################## AatII AccIII AclI AcsI AflII AflIII AgeI AhdI AocI Aor51HI ApoI AscI AseI AsnI Asp700I AspEI BalI BbeI BbrPI BcgI BclI BfrI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BsrBI BssHII Bst1107I BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV FbaI FseI HaeII HindIII HpaI KasI KspI MaeII MamI MfeI MluI MluNI MroI MscI MslI MunI NaeI NarI NdeI NgoMIV NotI NruI PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SgrAI SnaBI SpeI Sse8387I SspI SwaI XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### Acc65I 1596 GGTACC G^GTACC AccI 646 GTCTAC GT^[AC][GT]AC AccII 835 CGCG CG^CG AcyI 712 GGCGTC G[GA]^CG[CT]C Asp718I 1596 GGTACC G^GTACC AspI 258 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 788 TGCGCA TGC^GCA AvrII 93 CCTAGG C^CTAGG BamHI 1019 GGATCC G^GATCC BbvCI 311 CCTCAGC CCTCAGC(-5/-2) BglII 635 AGATCT A^GATCT BlnI 93 CCTAGG C^CTAGG BsaHI 712 GGCGTC G[GA]^CG[CT]C BsaI 427 GAGACC GGTCTC(1/5) BsiEI 763 CGGTCG CG[GA][CT]^CG Bsp1407I 689 TGTACA T^GTACA BsrDI 1535 CATTGC GCAATG(2/0) BsrFI 539 ACCGGC [GA]^CCGG[CT] BsrGI 689 TGTACA T^GTACA BssSI 683 CACGAG CACGAG(-5/-1) BstBI 1038 TTCGAA TT^CGAA BstUI 835 CGCG CG^CG Cfr10I 539 ACCGGC [GA]^CCGG[CT] DraIII 1331 CACTTAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 16 GACACTGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 541 CGGCCA [CT]^GGCC[GA] EcoT22I 595 ATGCAT ATGCA^T FspI 788 TGCGCA TGC^GCA HgaI 713 GCGTC GACGC(5/10) Hin1I 712 GGCGTC G[GA]^CG[CT]C HincII 188 GTCAAC GT[CT]^[GA]AC HindII 188 GTCAAC GT[CT]^[GA]AC KpnI 1596 GGTACC GGTAC^C MseI 1462 TTAA T^TAA MvnI 835 CGCG CG^CG NheI 1026 GCTAGC G^CTAGC NsiI 595 ATGCAT ATGCA^T NspV 1038 TTCGAA TT^CGAA PflFI 258 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC ScaI 783 AGTACT AGT^ACT SfuI 1038 TTCGAA TT^CGAA SmlI 520 CTCAAG C^T[CT][GA]AG SphI 889 GCATGC GCATG^C SspBI 689 TGTACA T^GTACA Tru9I 1462 TTAA T^TAA Tsp509I 1158 AATT ^AATT Tth111I 258 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### Alw44I 973 GTGCAC G^TGCAC 1321 GTGCAC ApaLI 973 GTGCAC G^TGCAC 1321 GTGCAC AspHI 973 GTGCAC G[AT]GC[AT]^C 1321 GTGCAC AvaI 406 CCCGGG C^[CT]CG[GA]G 1211 CCCGGG BanI 379 GGCACC G^G[CT][GA]CC 1596 GGTACC BbsI 255 GAAGAC GAAGAC(2/6) 958 GTCTTC BciVI 553 GGATAC GTATCC(6/5) 1385 GTATCC BglI 296 GCCGCAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1188 GCCACCACGGC BpuAI 255 GAAGAC GAAGAC(2/6) 958 GTCTTC BsgI 631 GTGCAG GTGCAG(16/14) 1165 GTGCAG BsiHKAI 973 GTGCAC G[AT]GC[AT]^C 1321 GTGCAC BsoBI 406 CCCGGG C^[CT]CG[GA]G 1211 CCCGGG BstAPI 501 GCAGGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 884 GCACAGCATGC EarI 754 CTCTTC CTCTTC(1/4) 952 GAAGAG FauI 414 CCCGC CCCGC(4/6) 1076 CCCGC HphI 172 GGTGA GGTGA(8/7) 481 TCACC Ksp632I 754 CTCTTC CTCTTC(1/4) 952 GAAGAG MspA1I 943 CAGCTG C[AC]G^C[GT]G 1114 CAGCTG NcoI 1227 CCATGG C^CATGG 1602 CCATGG PvuII 943 CAGCTG CAG^CTG 1114 CAGCTG SmaI 406 CCCGGG CCC^GGG 1211 CCCGGG StuI 524 AGGCCT AGG^CCT 614 AGGCCT XmaCI 406 CCCGGG C^CCGGG 1211 CCCGGG XmaI 406 CCCGGG C^CCGGG 1211 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 79 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI ApaI AvaII BanII BbvI BcnI BfaI BmyI BpmI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MaeI MaeIII MboI MboII MflI MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI TseI Tsp45I TspRI TthHB8I Van91I XhoII