################################## # NO CUT ENZYMES ( 124 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AgeI Alw44I Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BglI BglII BlnI BsaBI BsaHI BseAI BsgI BsiEI BsiWI BsoBI Bsp1407I BspEI BspHI BspLU11I BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstPI BstUI Cfr10I CpoI DraIII EagI EclXI Eco47III Eco52I EcoO65I FauI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MslI MspA1I MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SwaI Tth111I Van91I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AflII 2003 CTTAAG C^TTAAG AflIII 2371 ACGTGT A^C[GA][CT]GT AhdI 729 GACCGGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 2784 CCTAAGG CC^T[ACGT]AGG AseI 1283 ATTAAT AT^TAAT AsnI 1283 ATTAAT AT^TAAT AspEI 729 GACCGGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 2302 TGGCCA TGG^CCA BbsI 1034 GAAGAC GAAGAC(2/6) BfrI 2003 CTTAAG C^TTAAG BlpI 474 GCTCAGC GC^T[ACGT]AGC Bpu1102I 474 GCTCAGC GC^T[ACGT]AGC BpuAI 1034 GAAGAC GAAGAC(2/6) BsaWI 1494 ACCGGA [AT]^CCGG[AT] BspDI 1450 ATCGAT AT^CGAT BspMI 2097 ACCTGC ACCTGC(4/8) BsrBI 98 GAGCGG CCGCTC(-3/-3) Bst1107I 1920 GTATAC GTA^TAC BstZ17I 1920 GTATAC GTA^TAC Bsu36I 2784 CCTAAGG CC^T[ACGT]AGG CelII 474 GCTCAGC GC^T[ACGT]AGC ClaI 1450 ATCGAT AT^CGAT DraII 674 AGGACCC [GA]G^G[ACGT]CC[CT] DrdI 676 GACCCAAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 2299 CCATGG C^C[GA][CT]GG EaeI 2302 TGGCCA [CT]^GGCC[GA] Eam1105I 729 GACCGGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 2784 CCTAAGG CC^T[ACGT]AGG EcoNI 1658 CCTAATGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 674 AGGACCC [GA]G^G[ACGT]CC[CT] EcoRI 1083 GAATTC G^AATTC EcoRV 1763 GATATC GAT^ATC EcoT22I 2892 ATGCAT ATGCA^T MluNI 2302 TGGCCA TGG^CCA MscI 2302 TGGCCA TGG^CCA NcoI 2299 CCATGG C^CATGG NsiI 2892 ATGCAT ATGCA^T NspI 793 ACATGC [GA]CATG^[CT] PpuMI 674 AGGACCC [GA]G^G[AT]CC[CT] PshBI 1283 ATTAAT AT^TAAT SacI 266 GAGCTC GAGCT^C ScaI 1942 AGTACT AGT^ACT SfcI 1954 CTACAG C^T[GA][CT]AG XbaI 1688 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccI 1920 GTATAC GT^[AC][GT]AC 1952 GTCTAC AciI 585 CCGC CCGC(-3/-1) 100 GCGG AlwNI 1463 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG 2271 CAGCTTCTG BanII 266 GAGCTC G[GA]GC[CT]^C 722 GGGCTC BclI 1302 TGATCA T^GATCA 2947 TGATCA BsaAI 1482 TACGTG [CT]AC^GT[GA] 2370 TACGTG BsaI 890 GAGACC GGTCTC(1/5) 1959 GGTCTC BseRI 881 CTCCTC GAGGAG(10/8) 553 GAGGAG BsmBI 594 GAGACG CGTCTC(1/5) 1061 GAGACG BsmFI 2279 GGGAC GGGAC(10/14) 917 GTCCC BstYI 320 AGATCC [GA]^GATC[CT] 2046 AGATCC CfoI 1592 GCGC GCG^C 2101 GCGC EcoT14I 679 CCAAGG C^C[AT][AT]GG 2299 CCATGG FbaI 1302 TGATCA T^GATCA 2947 TGATCA HhaI 1592 GCGC GCG^C 2101 GCGC HinP1I 1592 GCGC G^CGC 2101 GCGC MflI 320 AGATCC [GA]^GATC[CT] 2046 AGATCC SmlI 1029 CTCAAG C^T[CT][GA]AG 2003 CTTAAG StuI 1655 AGGCCT AGG^CCT 2287 AGGCCT StyI 679 CCAAGG C^C[AT][AT]GG 2299 CCATGG TaqI 1451 TCGA T^CGA 2923 TCGA TthHB8I 1451 TCGA T^CGA 2923 TCGA XhoII 320 AGATCC [GA]^GATC[CT] 2046 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 67 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI AspHI AvaII BbvI BcnI BfaI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmAI BsmI Bsp1286I BsrI BssKI BstF5I BstNI BstXI Cac8I Cfr13I DdeI DpnII DraI EarI EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SspI TfiI Tru9I TseI Tsp45I Tsp509I TspRI