##################################
# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI Alw44I Aor51HI ApaI
ApaLI AscI Asp700I Asp718I AspI
AvaI AviII AvrII BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BglI BglII BlnI BsaBI BsaHI
BseAI BsgI BsiEI BsiWI BsoBI
Bsp1407I BspEI BspHI BspLU11I BsrDI
BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstPI BstUI Cfr10I
CpoI DraIII EagI EclXI Eco47III
Eco52I EcoO65I FauI FseI FspI
HaeII HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MslI
MspA1I MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SwaI Tth111I Van91I XcmI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AflII 2003 CTTAAG C^TTAAG
AflIII 2371 ACGTGT A^C[GA][CT]GT
AhdI 729 GACCGGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2784 CCTAAGG CC^T[ACGT]AGG
AseI 1283 ATTAAT AT^TAAT
AsnI 1283 ATTAAT AT^TAAT
AspEI 729 GACCGGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 2302 TGGCCA TGG^CCA
BbsI 1034 GAAGAC GAAGAC(2/6)
BfrI 2003 CTTAAG C^TTAAG
BlpI 474 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 474 GCTCAGC GC^T[ACGT]AGC
BpuAI 1034 GAAGAC GAAGAC(2/6)
BsaWI 1494 ACCGGA [AT]^CCGG[AT]
BspDI 1450 ATCGAT AT^CGAT
BspMI 2097 ACCTGC ACCTGC(4/8)
BsrBI 98 GAGCGG CCGCTC(-3/-3)
Bst1107I 1920 GTATAC GTA^TAC
BstZ17I 1920 GTATAC GTA^TAC
Bsu36I 2784 CCTAAGG CC^T[ACGT]AGG
CelII 474 GCTCAGC GC^T[ACGT]AGC
ClaI 1450 ATCGAT AT^CGAT
DraII 674 AGGACCC [GA]G^G[ACGT]CC[CT]
DrdI 676 GACCCAAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 2299 CCATGG C^C[GA][CT]GG
EaeI 2302 TGGCCA [CT]^GGCC[GA]
Eam1105I 729 GACCGGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2784 CCTAAGG CC^T[ACGT]AGG
EcoNI 1658 CCTAATGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 674 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 1083 GAATTC G^AATTC
EcoRV 1763 GATATC GAT^ATC
EcoT22I 2892 ATGCAT ATGCA^T
MluNI 2302 TGGCCA TGG^CCA
MscI 2302 TGGCCA TGG^CCA
NcoI 2299 CCATGG C^CATGG
NsiI 2892 ATGCAT ATGCA^T
NspI 793 ACATGC [GA]CATG^[CT]
PpuMI 674 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 1283 ATTAAT AT^TAAT
SacI 266 GAGCTC GAGCT^C
ScaI 1942 AGTACT AGT^ACT
SfcI 1954 CTACAG C^T[GA][CT]AG
XbaI 1688 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 1920 GTATAC GT^[AC][GT]AC
1952 GTCTAC
AciI 585 CCGC CCGC(-3/-1)
100 GCGG
AlwNI 1463 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
2271 CAGCTTCTG
BanII 266 GAGCTC G[GA]GC[CT]^C
722 GGGCTC
BclI 1302 TGATCA T^GATCA
2947 TGATCA
BsaAI 1482 TACGTG [CT]AC^GT[GA]
2370 TACGTG
BsaI 890 GAGACC GGTCTC(1/5)
1959 GGTCTC
BseRI 881 CTCCTC GAGGAG(10/8)
553 GAGGAG
BsmBI 594 GAGACG CGTCTC(1/5)
1061 GAGACG
BsmFI 2279 GGGAC GGGAC(10/14)
917 GTCCC
BstYI 320 AGATCC [GA]^GATC[CT]
2046 AGATCC
CfoI 1592 GCGC GCG^C
2101 GCGC
EcoT14I 679 CCAAGG C^C[AT][AT]GG
2299 CCATGG
FbaI 1302 TGATCA T^GATCA
2947 TGATCA
HhaI 1592 GCGC GCG^C
2101 GCGC
HinP1I 1592 GCGC G^CGC
2101 GCGC
MflI 320 AGATCC [GA]^GATC[CT]
2046 AGATCC
SmlI 1029 CTCAAG C^T[CT][GA]AG
2003 CTTAAG
StuI 1655 AGGCCT AGG^CCT
2287 AGGCCT
StyI 679 CCAAGG C^C[AT][AT]GG
2299 CCATGG
TaqI 1451 TCGA T^CGA
2923 TCGA
TthHB8I 1451 TCGA T^CGA
2923 TCGA
XhoII 320 AGATCC [GA]^GATC[CT]
2046 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AspHI AvaII BbvI BcnI BfaI
BmyI BpmI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmI Bsp1286I BsrI
BssKI BstF5I BstNI BstXI Cac8I
Cfr13I DdeI DpnII DraI EarI
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SspI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI