################################## # NO CUT ENZYMES ( 109 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AgeI AhdI AlwI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BamHI BbeI BbvCI BcgI BciVI BfrI BglII BlnI BsaBI BsaHI BseAI BsgI BsiEI BsiWI BsmI BspDI BspEI BspHI BspLU11I BsrDI BssHII Bst1107I BstAPI BstBI BstYI BstZ17I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I EcoNI EcoRV EcoT22I FauI FseI FspI Hin1I HincII HindII HpaI KasI KpnI KspI MamI MfeI MflI MluI MroI MseI MunI NarI NcoI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspI SwaI Tru9I Tth111I XbaI XhoI XhoII ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccII 65 CGCG CG^CG AcsI 1185 GAATTC [GA]^AATT[CT] AflIII 865 ACACGT A^C[GA][CT]GT Alw44I 1439 GTGCAC G^TGCAC AocI 1873 CCTGAGG CC^T[ACGT]AGG Aor51HI 121 AGCGCT AGC^GCT ApaLI 1439 GTGCAC G^TGCAC ApoI 1185 GAATTC [GA]^AATT[CT] Asp700I 1185 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BanI 1264 GGCACC G^G[CT][GA]CC BclI 192 TGATCA T^GATCA BfaI 1286 CTAG C^TAG BlpI 1779 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1779 GCTGAGC GC^T[ACGT]AGC BsaI 1984 GGTCTC GGTCTC(1/5) BsaWI 1578 TCCGGT [AT]^CCGG[AT] BsmBI 542 GAGACG CGTCTC(1/5) BsrBI 670 GAGCGG CCGCTC(-3/-3) BsrFI 365 GCCGGC [GA]^CCGG[CT] BstUI 65 CGCG CG^CG Bsu36I 1873 CCTGAGG CC^T[ACGT]AGG CelII 1779 GCTGAGC GC^T[ACGT]AGC Cfr10I 365 GCCGGC [GA]^CCGG[CT] Eco47III 121 AGCGCT AGC^GCT Eco81I 1873 CCTGAGG CC^T[ACGT]AGG EcoRI 1185 GAATTC G^AATTC FbaI 192 TGATCA T^GATCA HaeII 121 AGCGCT [GA]GCGC^[CT] HindIII 485 AAGCTT A^AGCTT MaeI 1286 CTAG C^TAG MvnI 65 CGCG CG^CG NaeI 365 GCCGGC GCC^GGC NdeI 1700 CATATG CA^TATG NgoMIV 365 GCCGGC G^CCGGC PshAI 160 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 59 GAGCTC GAGCT^C ScaI 807 AGTACT AGT^ACT SfaNI 1954 GCATC GCATC(5/9) SmaI 569 CCCGGG CCC^GGG SmlI 523 CTTGAG C^T[CT][GA]AG SphI 1015 GCATGC GCATG^C TaqI 876 TCGA T^CGA Tsp509I 1186 AATT ^AATT TthHB8I 876 TCGA T^CGA XcmI 1796 CCAGTGCCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 569 CCCGGG C^CCGGG XmaI 569 CCCGGG C^CCGGG XmnI 1185 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### BalI 769 TGGCCA TGG^CCA 1822 TGGCCA BbrPI 827 CACGTG CAC^GTG 866 CACGTG BglI 1652 GCCCTCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1805 GCCTGGGCGGC BsaAI 827 CACGTG [CT]AC^GT[GA] 866 CACGTG Bsp1407I 39 TGTACA T^GTACA 402 TGTACA BsrGI 39 TGTACA T^GTACA 402 TGTACA BstXI 90 CCAGCGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1613 CCACCCTCCTGG DsaI 285 CCGTGG C^C[GA][CT]GG 1407 CCGTGG EaeI 769 TGGCCA [CT]^GGCC[GA] 1822 TGGCCA HgaI 160 GACGC GACGC(5/10) 544 GACGC MluNI 769 TGGCCA TGG^CCA 1822 TGGCCA MscI 769 TGGCCA TGG^CCA 1822 TGGCCA NspI 1015 GCATGC [GA]CATG^[CT] 1642 GCATGT PflMI 418 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1030 CCAGCGAATGG PmaCI 827 CACGTG CAC^GTG 866 CACGTG PmlI 827 CACGTG CAC^GTG 866 CACGTG PpuMI 642 AGGACCT [GA]G^G[AT]CC[CT] 762 GGGACCT SspBI 39 TGTACA T^GTACA 402 TGTACA Van91I 418 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1030 CCAGCGAATGG ######################################### # 3 OR MORE CUT ENZYMES ( 82 enzymes) # ######################################### AciI AfaI AluI AlwNI ApaI AspHI AvaI AvaII BanII BbsI BbvI BcnI BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BssSI BstEII BstF5I BstNI BstPI Cac8I CfoI Cfr13I DdeI DpnII DraII EarI EcoO109I EcoO65I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfcI StuI StyI TfiI TseI Tsp45I TspRI