##################################
# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI AlwI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BamHI BbeI
BbvCI BcgI BciVI BfrI BglII
BlnI BsaBI BsaHI BseAI BsgI
BsiEI BsiWI BsmI BspDI BspEI
BspHI BspLU11I BsrDI BssHII Bst1107I
BstAPI BstBI BstYI BstZ17I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco52I EcoNI EcoRV
EcoT22I FauI FseI FspI Hin1I
HincII HindII HpaI KasI KpnI
KspI MamI MfeI MflI MluI
MroI MseI MunI NarI NcoI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI Tru9I
Tth111I XbaI XhoI XhoII
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccII 65 CGCG CG^CG
AcsI 1185 GAATTC [GA]^AATT[CT]
AflIII 865 ACACGT A^C[GA][CT]GT
Alw44I 1439 GTGCAC G^TGCAC
AocI 1873 CCTGAGG CC^T[ACGT]AGG
Aor51HI 121 AGCGCT AGC^GCT
ApaLI 1439 GTGCAC G^TGCAC
ApoI 1185 GAATTC [GA]^AATT[CT]
Asp700I 1185 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 1264 GGCACC G^G[CT][GA]CC
BclI 192 TGATCA T^GATCA
BfaI 1286 CTAG C^TAG
BlpI 1779 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1779 GCTGAGC GC^T[ACGT]AGC
BsaI 1984 GGTCTC GGTCTC(1/5)
BsaWI 1578 TCCGGT [AT]^CCGG[AT]
BsmBI 542 GAGACG CGTCTC(1/5)
BsrBI 670 GAGCGG CCGCTC(-3/-3)
BsrFI 365 GCCGGC [GA]^CCGG[CT]
BstUI 65 CGCG CG^CG
Bsu36I 1873 CCTGAGG CC^T[ACGT]AGG
CelII 1779 GCTGAGC GC^T[ACGT]AGC
Cfr10I 365 GCCGGC [GA]^CCGG[CT]
Eco47III 121 AGCGCT AGC^GCT
Eco81I 1873 CCTGAGG CC^T[ACGT]AGG
EcoRI 1185 GAATTC G^AATTC
FbaI 192 TGATCA T^GATCA
HaeII 121 AGCGCT [GA]GCGC^[CT]
HindIII 485 AAGCTT A^AGCTT
MaeI 1286 CTAG C^TAG
MvnI 65 CGCG CG^CG
NaeI 365 GCCGGC GCC^GGC
NdeI 1700 CATATG CA^TATG
NgoMIV 365 GCCGGC G^CCGGC
PshAI 160 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 59 GAGCTC GAGCT^C
ScaI 807 AGTACT AGT^ACT
SfaNI 1954 GCATC GCATC(5/9)
SmaI 569 CCCGGG CCC^GGG
SmlI 523 CTTGAG C^T[CT][GA]AG
SphI 1015 GCATGC GCATG^C
TaqI 876 TCGA T^CGA
Tsp509I 1186 AATT ^AATT
TthHB8I 876 TCGA T^CGA
XcmI 1796 CCAGTGCCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 569 CCCGGG C^CCGGG
XmaI 569 CCCGGG C^CCGGG
XmnI 1185 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
BalI 769 TGGCCA TGG^CCA
1822 TGGCCA
BbrPI 827 CACGTG CAC^GTG
866 CACGTG
BglI 1652 GCCCTCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1805 GCCTGGGCGGC
BsaAI 827 CACGTG [CT]AC^GT[GA]
866 CACGTG
Bsp1407I 39 TGTACA T^GTACA
402 TGTACA
BsrGI 39 TGTACA T^GTACA
402 TGTACA
BstXI 90 CCAGCGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1613 CCACCCTCCTGG
DsaI 285 CCGTGG C^C[GA][CT]GG
1407 CCGTGG
EaeI 769 TGGCCA [CT]^GGCC[GA]
1822 TGGCCA
HgaI 160 GACGC GACGC(5/10)
544 GACGC
MluNI 769 TGGCCA TGG^CCA
1822 TGGCCA
MscI 769 TGGCCA TGG^CCA
1822 TGGCCA
NspI 1015 GCATGC [GA]CATG^[CT]
1642 GCATGT
PflMI 418 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1030 CCAGCGAATGG
PmaCI 827 CACGTG CAC^GTG
866 CACGTG
PmlI 827 CACGTG CAC^GTG
866 CACGTG
PpuMI 642 AGGACCT [GA]G^G[AT]CC[CT]
762 GGGACCT
SspBI 39 TGTACA T^GTACA
402 TGTACA
Van91I 418 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1030 CCAGCGAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AfaI AluI AlwNI ApaI
AspHI AvaI AvaII BanII BbsI
BbvI BcnI BmyI BpmI BpuAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BssSI BstEII BstF5I
BstNI BstPI Cac8I CfoI Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoO65I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MnlI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfcI StuI StyI TfiI TseI
Tsp45I TspRI