##################################
# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII AccII AclI AcyI AflII
AflIII AgeI AhdI Aor51HI AscI
AseI AsnI AspEI AspI AvrII
BalI BbeI BbrPI BclI BfrI
BlnI BsaBI BsaHI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspLU11I
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I ClaI
CpoI DraI DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI FbaI FseI HgaI Hin1I
HincII HindII HindIII HpaI KasI
KspI MamI MfeI MluI MluNI
MscI MunI MvnI NaeI NarI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SspBI SspI SwaI
Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
Acc65I 1878 GGTACC G^GTACC
AccI 1910 GTAGAC GT^[AC][GT]AC
AccIII 32 TCCGGA T^CCGGA
AcsI 2139 GAATTT [GA]^AATT[CT]
Alw44I 856 GTGCAC G^TGCAC
AlwNI 1270 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 421 CCTGAGG CC^T[ACGT]AGG
ApaI 56 GGGCCC GGGCC^C
ApaLI 856 GTGCAC G^TGCAC
ApoI 2139 GAATTT [GA]^AATT[CT]
Asp700I 807 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1878 GGTACC G^GTACC
AvaI 1010 CCCGGG C^[CT]CG[GA]G
AviII 458 TGCGCA TGC^GCA
BamHI 2184 GGATCC G^GATCC
BcgI 264 CGAGGCTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1625 GTATCC GTATCC(6/5)
BglII 929 AGATCT A^GATCT
BlpI 1527 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1527 GCTCAGC GC^T[ACGT]AGC
BsaAI 2112 TACGTG [CT]AC^GT[GA]
BsaWI 32 TCCGGA [AT]^CCGG[AT]
BseAI 32 TCCGGA T^CCGGA
BseRI 130 GAGGAG GAGGAG(10/8)
BsgI 211 GTGCAG GTGCAG(16/14)
BsoBI 1010 CCCGGG C^[CT]CG[GA]G
BspEI 32 TCCGGA T^CCGGA
BspHI 821 TCATGA T^CATGA
BsrDI 2157 CATTGC GCAATG(2/0)
BstAPI 213 GCAGCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 421 CCTGAGG CC^T[ACGT]AGG
CelII 1527 GCTCAGC GC^T[ACGT]AGC
DrdI 562 GACACTCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 225 CGGCCA [CT]^GGCC[GA]
Eco81I 421 CCTGAGG CC^T[ACGT]AGG
EcoRV 1408 GATATC GAT^ATC
EcoT22I 39 ATGCAT ATGCA^T
FspI 458 TGCGCA TGC^GCA
HaeII 2177 GGCGCT [GA]GCGC^[CT]
KpnI 1878 GGTACC GGTAC^C
MroI 32 TCCGGA T^CCGGA
NdeI 1399 CATATG CA^TATG
NsiI 39 ATGCAT ATGCA^T
PvuII 1471 CAGCTG CAG^CTG
RcaI 821 TCATGA T^CATGA
SacI 2217 GAGCTC GAGCT^C
SbfI 2093 CCTGCAGG CCTGCA^GG
SexAI 1478 ACCTGGT A^CC[AT]GGT
SmaI 1010 CCCGGG CCC^GGG
SphI 41 GCATGC GCATG^C
Sse8387I 2093 CCTGCAGG CCTGCA^GG
StuI 938 AGGCCT AGG^CCT
XmaCI 1010 CCCGGG C^CCGGG
XmaI 1010 CCCGGG C^CCGGG
XmnI 807 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
BglI 1749 GCCAACCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1984 GCCAGAGCGGC
BsaI 1482 GGTCTC GGTCTC(1/5)
1864 GGTCTC
BsrBI 151 CCGCTC CCGCTC(-3/-3)
1988 GAGCGG
BsrFI 1253 GCCGGT [GA]^CCGG[CT]
1263 GCCGGT
BstXI 1053 CCACCCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1347 CCAGAGCCTTGG
Cfr10I 1253 GCCGGT [GA]^CCGG[CT]
1263 GCCGGT
EarI 1506 CTCTTC CTCTTC(1/4)
1515 GAAGAG
Ksp632I 1506 CTCTTC CTCTTC(1/4)
1515 GAAGAG
MspA1I 724 CCGCTG C[AC]G^C[GT]G
1471 CAGCTG
PflMI 1083 CCAAATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1452 CCAAGGACTGG
PpuMI 417 GGGTCCT [GA]G^G[AT]CC[CT]
426 GGGTCCC
ScaI 110 AGTACT AGT^ACT
1644 AGTACT
Tsp509I 2126 AATT ^AATT
2140 AATT
Van91I 1083 CCAAATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1452 CCAAGGACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BanI BanII BbsI BbvCI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI Tru9I
TseI Tsp45I TspRI TthHB8I XcmI
XhoII