##################################
# NO CUT ENZYMES (  107 enzymes) #
##################################

Acc65I      AccII       AccIII      AclI        AflII       
Aor51HI     AscI        AseI        AsnI        Asp700I     
Asp718I     AviII       AvrII       BbeI        BbrPI       
BbvCI       BfaI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BseAI       BsiWI       BsmBI       BsmI        BspDI       
BspEI       BsrBI       BsrDI       BssHII      Bst1107I    
BstAPI      BstBI       BstUI       BstZ17I     CelII       
ClaI        CpoI        DraIII      DrdI        EagI        
EclXI       Eco47III    Eco52I      EcoRI       EcoRV       
EcoT22I     FseI        FspI        HpaI        KasI        
KpnI        KspI        MaeI        MamI        MluI        
MroI        MvnI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflMI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SwaI        TfiI        
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AatII        1794  GACGTC               GACGT^C
AccI         1797  GTCTAC               GT^[AC][GT]AC
AcsI          384  GAATTT               [GA]^AATT[CT]
AcyI         1794  GACGTC               G[GA]^CG[CT]C
AgeI         1625  ACCGGT               A^CCGGT
AhdI         1071  GACTTACTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        171  GTGCAC               G^TGCAC
AocI          804  CCTGAGG              CC^T[ACGT]AGG
ApaI         1835  GGGCCC               GGGCC^C
ApaLI         171  GTGCAC               G^TGCAC
ApoI          384  GAATTT               [GA]^AATT[CT]
AspEI        1071  GACTTACTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI          879  CTCGGG               C^[CT]CG[GA]G
BamHI         157  GGATCC               G^GATCC
BanII        1835  GGGCCC               G[GA]GC[CT]^C
BbsI         1331  GAAGAC               GAAGAC(2/6)
BcgI          830  CGACTCAGATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1106  GTATCC               GTATCC(6/5)
BclI         1048  TGATCA               T^GATCA
BcnI         1110  CCGGG                CC^[GC]GG
BpuAI        1331  GAAGAC               GAAGAC(2/6)
BsaHI        1794  GACGTC               G[GA]^CG[CT]C
BsaWI        1625  ACCGGT               [AT]^CCGG[AT]
BsiEI        1033  CGACCG               CG[GA][CT]^CG
BsoBI         879  CTCGGG               C^[CT]CG[GA]G
Bsp1407I       55  TGTACA               T^GTACA
BspLU11I     2014  ACATGT               A^CATGT
BsrFI        1625  ACCGGT               [GA]^CCGG[CT]
BsrGI          55  TGTACA               T^GTACA
BssSI         243  CACGAG               CACGAG(-5/-1)
BstEII       1206  GGTCACC              G^GT[ACGT]ACC
BstPI        1206  GGTCACC              G^GT[ACGT]ACC
Bsu36I        804  CCTGAGG              CC^T[ACGT]AGG
Cfr10I       1625  ACCGGT               [GA]^CCGG[CT]
DraI         1905  TTTAAA               TTT^AAA
Eam1105I     1071  GACTTACTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        804  CCTGAGG              CC^T[ACGT]AGG
EcoNI         311  CCTGCAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      1206  GGTCACC              G^GT[ACGT]ACC
FbaI         1048  TGATCA               T^GATCA
HgaI          746  GACGC                GACGC(5/10)
Hin1I        1794  GACGTC               G[GA]^CG[CT]C
HindIII       192  AAGCTT               A^AGCTT
MfeI          760  CAATTG               C^AATTG
MslI         1010  CATGACAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI          760  CAATTG               C^AATTG
NciI         1110  CCGGG                CC^[GC]GG
NcoI          430  CCATGG               C^CATGG
NspI         2014  ACATGT               [GA]CATG^[CT]
PinAI        1625  ACCGGT               A^CCGGT
PvuII        1767  CAGCTG               CAG^CTG
SspBI          55  TGTACA               T^GTACA
SspI         1683  AATATT               AAT^ATT
StuI          733  AGGCCT               AGG^CCT


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AflIII       1711  ACGTGT               A^C[GA][CT]GT
             2014  ACATGT              
AspHI         171  GTGCAC               G[AT]GC[AT]^C
              702  GTGCTC              
BalI          133  TGGCCA               TGG^CCA
             2009  TGGCCA              
BanI          679  GGCACC               G^G[CT][GA]CC
              922  GGCACC              
BsiHKAI       171  GTGCAC               G[AT]GC[AT]^C
              702  GTGCTC              
BstXI         328  CCAAATGTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1300  CCAGCCACATGG        
FauI         1348  CCCGC                CCCGC(4/6)
               29  GCGGG               
HaeII        1296  AGCGCC               [GA]GCGC^[CT]
             1553  GGCGCT              
HincII         21  GTTGAC               GT[CT]^[GA]AC
             1707  GTCAAC              
HindII         21  GTTGAC               GT[CT]^[GA]AC
             1707  GTCAAC              
MluNI         133  TGGCCA               TGG^CCA
             2009  TGGCCA              
MscI          133  TGGCCA               TGG^CCA
             2009  TGGCCA              
PstI          312  CTGCAG               CTGCA^G
             1770  CTGCAG              
SmlI         1094  CTTGAG               C^T[CT][GA]AG
             1197  CTTGAG              


#########################################
# 3 OR MORE CUT ENZYMES (   83 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AspI        AvaII       BbvI        BmyI        BpmI        
BsaI        BsaJI       BseRI       BsgI        BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BspHI       
BspMI       BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
EcoO109I    EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MspA1I      MspI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       PflFI       PleI        
PpuMI       RcaI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        StyI        TaqI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
Tth111I     TthHB8I     XhoII