################################## # NO CUT ENZYMES ( 107 enzymes) # ################################## Acc65I AccII AccIII AclI AflII Aor51HI AscI AseI AsnI Asp700I Asp718I AviII AvrII BbeI BbrPI BbvCI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BseAI BsiWI BsmBI BsmI BspDI BspEI BsrBI BsrDI BssHII Bst1107I BstAPI BstBI BstUI BstZ17I CelII ClaI CpoI DraIII DrdI EagI EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FseI FspI HpaI KasI KpnI KspI MaeI MamI MluI MroI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SwaI TfiI Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AatII 1794 GACGTC GACGT^C AccI 1797 GTCTAC GT^[AC][GT]AC AcsI 384 GAATTT [GA]^AATT[CT] AcyI 1794 GACGTC G[GA]^CG[CT]C AgeI 1625 ACCGGT A^CCGGT AhdI 1071 GACTTACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 171 GTGCAC G^TGCAC AocI 804 CCTGAGG CC^T[ACGT]AGG ApaI 1835 GGGCCC GGGCC^C ApaLI 171 GTGCAC G^TGCAC ApoI 384 GAATTT [GA]^AATT[CT] AspEI 1071 GACTTACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 879 CTCGGG C^[CT]CG[GA]G BamHI 157 GGATCC G^GATCC BanII 1835 GGGCCC G[GA]GC[CT]^C BbsI 1331 GAAGAC GAAGAC(2/6) BcgI 830 CGACTCAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1106 GTATCC GTATCC(6/5) BclI 1048 TGATCA T^GATCA BcnI 1110 CCGGG CC^[GC]GG BpuAI 1331 GAAGAC GAAGAC(2/6) BsaHI 1794 GACGTC G[GA]^CG[CT]C BsaWI 1625 ACCGGT [AT]^CCGG[AT] BsiEI 1033 CGACCG CG[GA][CT]^CG BsoBI 879 CTCGGG C^[CT]CG[GA]G Bsp1407I 55 TGTACA T^GTACA BspLU11I 2014 ACATGT A^CATGT BsrFI 1625 ACCGGT [GA]^CCGG[CT] BsrGI 55 TGTACA T^GTACA BssSI 243 CACGAG CACGAG(-5/-1) BstEII 1206 GGTCACC G^GT[ACGT]ACC BstPI 1206 GGTCACC G^GT[ACGT]ACC Bsu36I 804 CCTGAGG CC^T[ACGT]AGG Cfr10I 1625 ACCGGT [GA]^CCGG[CT] DraI 1905 TTTAAA TTT^AAA Eam1105I 1071 GACTTACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 804 CCTGAGG CC^T[ACGT]AGG EcoNI 311 CCTGCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1206 GGTCACC G^GT[ACGT]ACC FbaI 1048 TGATCA T^GATCA HgaI 746 GACGC GACGC(5/10) Hin1I 1794 GACGTC G[GA]^CG[CT]C HindIII 192 AAGCTT A^AGCTT MfeI 760 CAATTG C^AATTG MslI 1010 CATGACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 760 CAATTG C^AATTG NciI 1110 CCGGG CC^[GC]GG NcoI 430 CCATGG C^CATGG NspI 2014 ACATGT [GA]CATG^[CT] PinAI 1625 ACCGGT A^CCGGT PvuII 1767 CAGCTG CAG^CTG SspBI 55 TGTACA T^GTACA SspI 1683 AATATT AAT^ATT StuI 733 AGGCCT AGG^CCT ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AflIII 1711 ACGTGT A^C[GA][CT]GT 2014 ACATGT AspHI 171 GTGCAC G[AT]GC[AT]^C 702 GTGCTC BalI 133 TGGCCA TGG^CCA 2009 TGGCCA BanI 679 GGCACC G^G[CT][GA]CC 922 GGCACC BsiHKAI 171 GTGCAC G[AT]GC[AT]^C 702 GTGCTC BstXI 328 CCAAATGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1300 CCAGCCACATGG FauI 1348 CCCGC CCCGC(4/6) 29 GCGGG HaeII 1296 AGCGCC [GA]GCGC^[CT] 1553 GGCGCT HincII 21 GTTGAC GT[CT]^[GA]AC 1707 GTCAAC HindII 21 GTTGAC GT[CT]^[GA]AC 1707 GTCAAC MluNI 133 TGGCCA TGG^CCA 2009 TGGCCA MscI 133 TGGCCA TGG^CCA 2009 TGGCCA PstI 312 CTGCAG CTGCA^G 1770 CTGCAG SmlI 1094 CTTGAG C^T[CT][GA]AG 1197 CTTGAG ######################################### # 3 OR MORE CUT ENZYMES ( 83 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AspI AvaII BbvI BmyI BpmI BsaI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BspHI BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PflFI PleI PpuMI RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XhoII