##################################
# NO CUT ENZYMES ( 107 enzymes) #
##################################
Acc65I AccII AccIII AclI AflII
Aor51HI AscI AseI AsnI Asp700I
Asp718I AviII AvrII BbeI BbrPI
BbvCI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BseAI BsiWI BsmBI BsmI BspDI
BspEI BsrBI BsrDI BssHII Bst1107I
BstAPI BstBI BstUI BstZ17I CelII
ClaI CpoI DraIII DrdI EagI
EclXI Eco47III Eco52I EcoRI EcoRV
EcoT22I FseI FspI HpaI KasI
KpnI KspI MaeI MamI MluI
MroI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SwaI TfiI
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AatII 1794 GACGTC GACGT^C
AccI 1797 GTCTAC GT^[AC][GT]AC
AcsI 384 GAATTT [GA]^AATT[CT]
AcyI 1794 GACGTC G[GA]^CG[CT]C
AgeI 1625 ACCGGT A^CCGGT
AhdI 1071 GACTTACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 171 GTGCAC G^TGCAC
AocI 804 CCTGAGG CC^T[ACGT]AGG
ApaI 1835 GGGCCC GGGCC^C
ApaLI 171 GTGCAC G^TGCAC
ApoI 384 GAATTT [GA]^AATT[CT]
AspEI 1071 GACTTACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 879 CTCGGG C^[CT]CG[GA]G
BamHI 157 GGATCC G^GATCC
BanII 1835 GGGCCC G[GA]GC[CT]^C
BbsI 1331 GAAGAC GAAGAC(2/6)
BcgI 830 CGACTCAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1106 GTATCC GTATCC(6/5)
BclI 1048 TGATCA T^GATCA
BcnI 1110 CCGGG CC^[GC]GG
BpuAI 1331 GAAGAC GAAGAC(2/6)
BsaHI 1794 GACGTC G[GA]^CG[CT]C
BsaWI 1625 ACCGGT [AT]^CCGG[AT]
BsiEI 1033 CGACCG CG[GA][CT]^CG
BsoBI 879 CTCGGG C^[CT]CG[GA]G
Bsp1407I 55 TGTACA T^GTACA
BspLU11I 2014 ACATGT A^CATGT
BsrFI 1625 ACCGGT [GA]^CCGG[CT]
BsrGI 55 TGTACA T^GTACA
BssSI 243 CACGAG CACGAG(-5/-1)
BstEII 1206 GGTCACC G^GT[ACGT]ACC
BstPI 1206 GGTCACC G^GT[ACGT]ACC
Bsu36I 804 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1625 ACCGGT [GA]^CCGG[CT]
DraI 1905 TTTAAA TTT^AAA
Eam1105I 1071 GACTTACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 804 CCTGAGG CC^T[ACGT]AGG
EcoNI 311 CCTGCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1206 GGTCACC G^GT[ACGT]ACC
FbaI 1048 TGATCA T^GATCA
HgaI 746 GACGC GACGC(5/10)
Hin1I 1794 GACGTC G[GA]^CG[CT]C
HindIII 192 AAGCTT A^AGCTT
MfeI 760 CAATTG C^AATTG
MslI 1010 CATGACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 760 CAATTG C^AATTG
NciI 1110 CCGGG CC^[GC]GG
NcoI 430 CCATGG C^CATGG
NspI 2014 ACATGT [GA]CATG^[CT]
PinAI 1625 ACCGGT A^CCGGT
PvuII 1767 CAGCTG CAG^CTG
SspBI 55 TGTACA T^GTACA
SspI 1683 AATATT AAT^ATT
StuI 733 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AflIII 1711 ACGTGT A^C[GA][CT]GT
2014 ACATGT
AspHI 171 GTGCAC G[AT]GC[AT]^C
702 GTGCTC
BalI 133 TGGCCA TGG^CCA
2009 TGGCCA
BanI 679 GGCACC G^G[CT][GA]CC
922 GGCACC
BsiHKAI 171 GTGCAC G[AT]GC[AT]^C
702 GTGCTC
BstXI 328 CCAAATGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1300 CCAGCCACATGG
FauI 1348 CCCGC CCCGC(4/6)
29 GCGGG
HaeII 1296 AGCGCC [GA]GCGC^[CT]
1553 GGCGCT
HincII 21 GTTGAC GT[CT]^[GA]AC
1707 GTCAAC
HindII 21 GTTGAC GT[CT]^[GA]AC
1707 GTCAAC
MluNI 133 TGGCCA TGG^CCA
2009 TGGCCA
MscI 133 TGGCCA TGG^CCA
2009 TGGCCA
PstI 312 CTGCAG CTGCA^G
1770 CTGCAG
SmlI 1094 CTTGAG C^T[CT][GA]AG
1197 CTTGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspI AvaII BbvI BmyI BpmI
BsaI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI Bsp1286I BspHI
BspMI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV PflFI PleI
PpuMI RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I XhoII