################################## # NO CUT ENZYMES ( 117 enzymes) # ################################## AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspHI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BciVI BfrI BlnI BlpI BpmI Bpu1102I BsaAI BsaI BseAI BsiHKAI BsiWI BsmBI BsmI BspDI BspEI BspLU11I BsrBI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraIII Eam1105I Eco47III Eco81I EcoO65I EcoRI EcoRV FseI FspI HaeII HgaI HincII HindII HindIII HpaI KasI KspI MfeI MluI MluNI MroI MscI MunI NarI NdeI NheI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfoI SfuI SnaBI SpeI SphI Sse8387I SspI SwaI XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 29 enzymes) # ################################### AatII 536 GACGTC GACGT^C Acc65I 1192 GGTACC G^GTACC AcyI 536 GACGTC G[GA]^CG[CT]C AlwNI 1268 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG Asp718I 1192 GGTACC G^GTACC BclI 1418 TGATCA T^GATCA BsaBI 802 GATTTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 536 GACGTC G[GA]^CG[CT]C Bsp1407I 1119 TGTACA T^GTACA BsrGI 1119 TGTACA T^GTACA DdeI 578 CTCAG C^T[ACGT]AG DraI 1356 TTTAAA TTT^AAA DraII 638 GGGTCCC [GA]G^G[ACGT]CC[CT] DrdI 536 GACGTCCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO109I 638 GGGTCCC [GA]G^G[ACGT]CC[CT] FbaI 1418 TGATCA T^GATCA Hin1I 536 GACGTC G[GA]^CG[CT]C KpnI 1192 GGTACC GGTAC^C MamI 802 GATTTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC PflMI 711 CCATGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 638 GGGTCCC [GA]G^G[AT]CC[CT] PvuII 580 CAGCTG CAG^CTG SgrAI 116 CGCCGGCG C[GA]^CCGG[CT]G SmlI 1 CTTGAG C^T[CT][GA]AG SspBI 1119 TGTACA T^GTACA StuI 1424 AGGCCT AGG^CCT TaqI 1302 TCGA T^CGA TthHB8I 1302 TCGA T^CGA Van91I 711 CCATGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AlwI 853 GATCC GGATC(4/5) 1003 GATCC AspI 936 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC 1162 GACTGGGTC BbsI 356 GAAGAC GAAGAC(2/6) 971 GTCTTC BfaI 1197 CTAG C^TAG 1247 CTAG BglII 700 AGATCT A^GATCT 1179 AGATCT BpuAI 356 GAAGAC GAAGAC(2/6) 971 GTCTTC BsaWI 108 ACCGGA [AT]^CCGG[AT] 191 ACCGGA BsmAI 1011 GAGAC GTCTC(1/5) 1362 GAGAC BspHI 787 TCATGA T^CATGA 907 TCATGA BsrDI 531 GCAATG GCAATG(2/0) 549 GCAATG EarI 368 CTCTTC CTCTTC(1/4) 592 GAAGAG EcoNI 754 CCTGCCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1416 CCTGATCAAGG EcoT22I 858 ATGCAT ATGCA^T 879 ATGCAT Ksp632I 368 CTCTTC CTCTTC(1/4) 592 GAAGAG MaeI 1197 CTAG C^TAG 1247 CTAG MaeII 537 ACGT A^CGT 1037 ACGT MspA1I 148 CAGCGG C[AC]G^C[GT]G 580 CAGCTG NcoI 621 CCATGG C^CATGG 711 CCATGG NsiI 858 ATGCAT ATGCA^T 879 ATGCAT NspI 435 ACATGC [GA]CATG^[CT] 1116 GCATGT PflFI 936 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC 1162 GACTGGGTC PleI 1284 GACTC GAGTC(4/5) 1364 GACTC PstI 927 CTGCAG CTGCA^G 1031 CTGCAG RcaI 787 TCATGA T^CATGA 907 TCATGA SfiI 136 GGCCGGCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 163 GGCCGGCGCGGCC SmaI 10 CCCGGG CCC^GGG 441 CCCGGG Tth111I 936 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC 1162 GACTGGGTC XmaCI 10 CCCGGG C^CCGGG 441 CCCGGG XmaI 10 CCCGGG C^CCGGG 441 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 83 enzymes) # ######################################### AccII AciI AfaI AluI AvaI AvaII BanI BanII BbvI BcnI BglI BmyI BsaJI BseRI BsgI BsiEI BsiYI BslI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DpnII DsaI EaeI EagI EclXI Eco52I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII