##################################
# NO CUT ENZYMES ( 117 enzymes) #
##################################
AccI AccIII AclI AcsI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AspHI
AviII AvrII BalI BamHI BbeI
BbrPI BbvCI BcgI BciVI BfrI
BlnI BlpI BpmI Bpu1102I BsaAI
BsaI BseAI BsiHKAI BsiWI BsmBI
BsmI BspDI BspEI BspLU11I BsrBI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraIII Eam1105I
Eco47III Eco81I EcoO65I EcoRI EcoRV
FseI FspI HaeII HgaI HincII
HindII HindIII HpaI KasI KspI
MfeI MluI MluNI MroI MscI
MunI NarI NdeI NheI NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfoI SfuI SnaBI SpeI SphI
Sse8387I SspI SwaI XbaI XcmI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AatII 536 GACGTC GACGT^C
Acc65I 1192 GGTACC G^GTACC
AcyI 536 GACGTC G[GA]^CG[CT]C
AlwNI 1268 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 1192 GGTACC G^GTACC
BclI 1418 TGATCA T^GATCA
BsaBI 802 GATTTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 536 GACGTC G[GA]^CG[CT]C
Bsp1407I 1119 TGTACA T^GTACA
BsrGI 1119 TGTACA T^GTACA
DdeI 578 CTCAG C^T[ACGT]AG
DraI 1356 TTTAAA TTT^AAA
DraII 638 GGGTCCC [GA]G^G[ACGT]CC[CT]
DrdI 536 GACGTCCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 638 GGGTCCC [GA]G^G[ACGT]CC[CT]
FbaI 1418 TGATCA T^GATCA
Hin1I 536 GACGTC G[GA]^CG[CT]C
KpnI 1192 GGTACC GGTAC^C
MamI 802 GATTTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PflMI 711 CCATGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 638 GGGTCCC [GA]G^G[AT]CC[CT]
PvuII 580 CAGCTG CAG^CTG
SgrAI 116 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 1 CTTGAG C^T[CT][GA]AG
SspBI 1119 TGTACA T^GTACA
StuI 1424 AGGCCT AGG^CCT
TaqI 1302 TCGA T^CGA
TthHB8I 1302 TCGA T^CGA
Van91I 711 CCATGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AlwI 853 GATCC GGATC(4/5)
1003 GATCC
AspI 936 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1162 GACTGGGTC
BbsI 356 GAAGAC GAAGAC(2/6)
971 GTCTTC
BfaI 1197 CTAG C^TAG
1247 CTAG
BglII 700 AGATCT A^GATCT
1179 AGATCT
BpuAI 356 GAAGAC GAAGAC(2/6)
971 GTCTTC
BsaWI 108 ACCGGA [AT]^CCGG[AT]
191 ACCGGA
BsmAI 1011 GAGAC GTCTC(1/5)
1362 GAGAC
BspHI 787 TCATGA T^CATGA
907 TCATGA
BsrDI 531 GCAATG GCAATG(2/0)
549 GCAATG
EarI 368 CTCTTC CTCTTC(1/4)
592 GAAGAG
EcoNI 754 CCTGCCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1416 CCTGATCAAGG
EcoT22I 858 ATGCAT ATGCA^T
879 ATGCAT
Ksp632I 368 CTCTTC CTCTTC(1/4)
592 GAAGAG
MaeI 1197 CTAG C^TAG
1247 CTAG
MaeII 537 ACGT A^CGT
1037 ACGT
MspA1I 148 CAGCGG C[AC]G^C[GT]G
580 CAGCTG
NcoI 621 CCATGG C^CATGG
711 CCATGG
NsiI 858 ATGCAT ATGCA^T
879 ATGCAT
NspI 435 ACATGC [GA]CATG^[CT]
1116 GCATGT
PflFI 936 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1162 GACTGGGTC
PleI 1284 GACTC GAGTC(4/5)
1364 GACTC
PstI 927 CTGCAG CTGCA^G
1031 CTGCAG
RcaI 787 TCATGA T^CATGA
907 TCATGA
SfiI 136 GGCCGGCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
163 GGCCGGCGCGGCC
SmaI 10 CCCGGG CCC^GGG
441 CCCGGG
Tth111I 936 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1162 GACTGGGTC
XmaCI 10 CCCGGG C^CCGGG
441 CCCGGG
XmaI 10 CCCGGG C^CCGGG
441 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccII AciI AfaI AluI AvaI
AvaII BanI BanII BbvI BcnI
BglI BmyI BsaJI BseRI BsgI
BsiEI BsiYI BslI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DpnII DsaI
EaeI EagI EclXI Eco52I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII