################################## # NO CUT ENZYMES ( 105 enzymes) # ################################## AatII AccI AccIII AcsI AcyI AflII Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I AspI AvrII BbeI BbrPI BcgI BciVI BclI BfrI BglII BlnI BsaAI BsaBI BsaHI BseAI BsiEI BsiWI BsmBI BspDI BspEI BspHI BspLU11I BsrBI BssHII Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I ClaI DraI DrdI EagI EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoT22I FbaI FseI Hin1I HincII HindII HindIII HpaI KasI Ksp632I KspI MamI MfeI MroI MunI NaeI NarI NdeI NgoMIV NheI NotI NsiI NspV PacI PflFI PmaCI PmeI PmlI PshAI PshBI PvuI RcaI SacII SalI SapI SbfI ScaI SexAI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspI SwaI Tsp509I Tth111I XbaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### Acc65I 1227 GGTACC G^GTACC AclI 2494 AACGTT AA^CGTT AgeI 93 ACCGGT A^CCGGT AhdI 2220 GACCCGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 1811 GGGCCC GGGCC^C Asp718I 1227 GGTACC G^GTACC AspEI 2220 GACCCGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 115 TGCGCA TGC^GCA BsaWI 93 ACCGGT [AT]^CCGG[AT] BsmI 298 GCATTC GAATGC(1/-1) Bsp1407I 48 TGTACA T^GTACA BspMI 1484 ACCTGC ACCTGC(4/8) BsrDI 2096 GCAATG GCAATG(2/0) BsrFI 93 ACCGGT [GA]^CCGG[CT] BsrGI 48 TGTACA T^GTACA BssSI 628 CTCGTG CACGAG(-5/-1) BstAPI 2479 GCACCAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Cfr10I 93 ACCGGT [GA]^CCGG[CT] CpoI 90 CGGACCG CG^G[AT]CCG Eam1105I 2220 GACCCGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 960 CCTCATCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 1726 GATATC GAT^ATC FauI 2222 CCCGC CCCGC(4/6) FspI 115 TGCGCA TGC^GCA KpnI 1227 GGTACC GGTAC^C MluI 43 ACGCGT A^CGCGT MseI 2540 TTAA T^TAA NruI 2208 TCGCGA TCG^CGA PaeR7I 1064 CTCGAG C^TCGAG PinAI 93 ACCGGT A^CCGGT PpuMI 1651 GGGACCC [GA]G^G[AT]CC[CT] Psp1406I 2494 AACGTT AA^CGTT RsrII 90 CGGACCG CG^G[AT]CCG SacI 1659 GAGCTC GAGCT^C SfiI 1462 GGCCCAGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SphI 112 GCATGC GCATG^C SspBI 48 TGTACA T^GTACA Tru9I 2540 TTAA T^TAA XcmI 156 CCACCATGTCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1064 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AflIII 43 ACGCGT A^C[GA][CT]GT 798 ACGTGT BamHI 213 GGATCC G^GATCC 698 GGATCC BbsI 1446 GTCTTC GAAGAC(2/6) 2393 GTCTTC BbvCI 259 GCTGAGG CCTCAGC(-5/-2) 1708 GCTGAGG BlpI 664 GCTCAGC GC^T[ACGT]AGC 832 GCTGAGC Bpu1102I 664 GCTCAGC GC^T[ACGT]AGC 832 GCTGAGC BpuAI 1446 GTCTTC GAAGAC(2/6) 2393 GTCTTC BsaI 1082 GAGACC GGTCTC(1/5) 1406 GAGACC BsgI 612 CTGCAC GTGCAG(16/14) 2081 CTGCAC CelII 664 GCTCAGC GC^T[ACGT]AGC 832 GCTGAGC DraIII 1377 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG 2480 CACCAAGTG MaeII 798 ACGT A^CGT 2495 ACGT SmlI 1064 CTCGAG C^T[CT][GA]AG 2317 CTCAAG StuI 305 AGGCCT AGG^CCT 1800 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 99 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BalI BanI BanII BbvI BcnI BfaI BglI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI StyI TaqI TfiI TseI Tsp45I TspRI TthHB8I Van91I XhoII XmaCI XmaI