##################################
# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII AccI AccIII AcsI AcyI
AflII Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
AspI AvrII BbeI BbrPI BcgI
BciVI BclI BfrI BglII BlnI
BsaAI BsaBI BsaHI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BspHI
BspLU11I BsrBI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I ClaI
DraI DrdI EagI EarI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoT22I FbaI FseI Hin1I HincII
HindII HindIII HpaI KasI Ksp632I
KspI MamI MfeI MroI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NsiI NspV PacI PflFI
PmaCI PmeI PmlI PshAI PshBI
PvuI RcaI SacII SalI SapI
SbfI ScaI SexAI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
SwaI Tsp509I Tth111I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 1227 GGTACC G^GTACC
AclI 2494 AACGTT AA^CGTT
AgeI 93 ACCGGT A^CCGGT
AhdI 2220 GACCCGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 1811 GGGCCC GGGCC^C
Asp718I 1227 GGTACC G^GTACC
AspEI 2220 GACCCGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 115 TGCGCA TGC^GCA
BsaWI 93 ACCGGT [AT]^CCGG[AT]
BsmI 298 GCATTC GAATGC(1/-1)
Bsp1407I 48 TGTACA T^GTACA
BspMI 1484 ACCTGC ACCTGC(4/8)
BsrDI 2096 GCAATG GCAATG(2/0)
BsrFI 93 ACCGGT [GA]^CCGG[CT]
BsrGI 48 TGTACA T^GTACA
BssSI 628 CTCGTG CACGAG(-5/-1)
BstAPI 2479 GCACCAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 93 ACCGGT [GA]^CCGG[CT]
CpoI 90 CGGACCG CG^G[AT]CCG
Eam1105I 2220 GACCCGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 960 CCTCATCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1726 GATATC GAT^ATC
FauI 2222 CCCGC CCCGC(4/6)
FspI 115 TGCGCA TGC^GCA
KpnI 1227 GGTACC GGTAC^C
MluI 43 ACGCGT A^CGCGT
MseI 2540 TTAA T^TAA
NruI 2208 TCGCGA TCG^CGA
PaeR7I 1064 CTCGAG C^TCGAG
PinAI 93 ACCGGT A^CCGGT
PpuMI 1651 GGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 2494 AACGTT AA^CGTT
RsrII 90 CGGACCG CG^G[AT]CCG
SacI 1659 GAGCTC GAGCT^C
SfiI 1462 GGCCCAGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 112 GCATGC GCATG^C
SspBI 48 TGTACA T^GTACA
Tru9I 2540 TTAA T^TAA
XcmI 156 CCACCATGTCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1064 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AflIII 43 ACGCGT A^C[GA][CT]GT
798 ACGTGT
BamHI 213 GGATCC G^GATCC
698 GGATCC
BbsI 1446 GTCTTC GAAGAC(2/6)
2393 GTCTTC
BbvCI 259 GCTGAGG CCTCAGC(-5/-2)
1708 GCTGAGG
BlpI 664 GCTCAGC GC^T[ACGT]AGC
832 GCTGAGC
Bpu1102I 664 GCTCAGC GC^T[ACGT]AGC
832 GCTGAGC
BpuAI 1446 GTCTTC GAAGAC(2/6)
2393 GTCTTC
BsaI 1082 GAGACC GGTCTC(1/5)
1406 GAGACC
BsgI 612 CTGCAC GTGCAG(16/14)
2081 CTGCAC
CelII 664 GCTCAGC GC^T[ACGT]AGC
832 GCTGAGC
DraIII 1377 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
2480 CACCAAGTG
MaeII 798 ACGT A^CGT
2495 ACGT
SmlI 1064 CTCGAG C^T[CT][GA]AG
2317 CTCAAG
StuI 305 AGGCCT AGG^CCT
1800 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BalI
BanI BanII BbvI BcnI BfaI
BglI BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI StyI TaqI
TfiI TseI Tsp45I TspRI TthHB8I
Van91I XhoII XmaCI XmaI